Aminoacid and vitamin body distribution: an additional use of the autographic method
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200 μm whole body sections of pre-treated laboratory animals were inoculated with vitamin- or aminoacid-dependent micro-organisms and incubated. Live micro-organisms are revealed by tetrazolium salts and furnish vitamin or aminoacid distribution maps. In particular methionine, cyanocobalamin and thiamine distribution is studied; to verify that maps record only these agents, specific antimetabolites were used (Friedrich's S-102 and α-methyl-methionine) 1.
The advantages of the method are that we obtain a general and comparative view in a single animal; in contrast to autoradiography the method is a biological one and gives information only of the “active substances”.
Key-wordsDrug Tissue Distribution Vitamins Aminoacids
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