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Aminoacid and vitamin body distribution: an additional use of the autographic method

  • E. Tubaro
  • M. J. Bulgini
Article
  • 19 Downloads

Summary

200 μm whole body sections of pre-treated laboratory animals were inoculated with vitamin- or aminoacid-dependent micro-organisms and incubated. Live micro-organisms are revealed by tetrazolium salts and furnish vitamin or aminoacid distribution maps. In particular methionine, cyanocobalamin and thiamine distribution is studied; to verify that maps record only these agents, specific antimetabolites were used (Friedrich's S-102 and α-methyl-methionine) 1.

The advantages of the method are that we obtain a general and comparative view in a single animal; in contrast to autoradiography the method is a biological one and gives information only of the “active substances”.

Key-words

Drug Tissue Distribution Vitamins Aminoacids 

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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • E. Tubaro
    • 1
  • M. J. Bulgini
    • 1
  1. 1.Research Department Dott. R. Ravasini and Co.Rome

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