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A complex interplay of positive and negative elements is responsible for the different transcriptional activity of liver NF1 variants

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Abstract

A full-length cDNA of the rat liver Nuclear Factor 1 (NF1L21) has been cloned and expressed inS. cerevisiae to analyse the architecture of its activation domain. NF1L21 displays a specific DNA-binding activity, as well as the ability to activate transcription from an artificial NF1-responsive promoter in yeast. Interaction of two or more NF1L21 molecules with multiple sites on the same promoter activated transcription in a synergistic fashion. Functional analysis of the activation domain of NF1L21 reveals a tripartite structure. Two distinct positive elements are required for NF1L21-mediated transcription activation. A proline-rich element sandwiched between these two positive domains attenuates their transactivation potential. A shorter NF1L variant (NF1L4) in which the distal positive element is replaced by a different sequence was also isolated. NF1L4 displays the same DNA-binding activity and dimerisation properties as NF1L21, but is unable to activate transcription in yeast.

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Authors and Affiliations

  1. Istituto di Ricerche di Biologia Molecolare P. Angeletti, Via Pontina km 30.600, 00040, Romezia (Roma), Italy

    Paolo Monaci, Maurizio Nuzzo & Alfredo Nicosia

  2. European Molecular Biology Laboratory, Meyerhofstrasse, 1, 6900, Heidelberg, Germany

    Susanne Stämpfli & David Tollervey

  3. Facoltà di Medicinae Chirurgia, II Università di Napoli, Via Pansini, 5, 80131, Napoli, Italy

    Vincenzo De Simone

Authors
  1. Paolo Monaci
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  2. Maurizio Nuzzo
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  3. Susanne Stämpfli
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  4. David Tollervey
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  5. Vincenzo De Simone
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  6. Alfredo Nicosia
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Monaci, P., Nuzzo, M., Stämpfli, S. et al. A complex interplay of positive and negative elements is responsible for the different transcriptional activity of liver NF1 variants. Mol Biol Rep 21, 147–158 (1995). https://doi.org/10.1007/BF00997237

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  • DOI: https://doi.org/10.1007/BF00997237

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