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Über die gestörte Glucuronidsynthese in der Leber diabetischer Ratten

Impaired formation of glucuronic acid conjugates in liver tissue of diabetic rats

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Summary

The synthesisin vitro of o-aminophenol-glucuronide by liver tissue has been investigated in diabetic rats. Insulin deficiency was induced by withdrawal of insulin in alloxan treated, insulin substituted rats or by injection of anti-insulinserum. The hepatic formation of o-aminophenol-glucuronide was strongly reduced under these conditions. This result could not be explained by differences in the activity of the UDP-glucuronyl-transferase. However, the concentration of UDP-glucuronic acid in liver tissue of the diabetic rats was also decreased to 50% of control values whereas the amount of UDPG was augmented. These findings are consistent with the observation that the activity of UDPG-dehydrogenase, the enzyme catalyzing the formation of UDP-glucuronic acid from UDPG, is reduced in the liver of diabetic or fasting rats. The activity of the UDPGA-pyrophosphatase which is responsible for inactivation of the nucleotide, has been found to be unchanged in the diabetic animals.

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Abbreviations

AIS:

Anti-Insulin-Serum

ATP:

Adenosin-triphosphat

NAD+ :

Nicotinamid-Adenin-Dinucleotid

oAP:

o-Aminophenol

UDPG:

Uridin-diphosphat-glucose

UDPGA:

Uridindiphosphat-Glucuronsäure

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der Universität Göttingen, Deutschland

    B. Müller-Oerlinghausen

  2. Dept. of Medical Sciences, Ministry of Public Health, Yod-se/Bangkok, Thailand

    B. Müller-Oerlinghausen

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  1. B. Müller-Oerlinghausen
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Ein Teil der Ergebnisse wurde auf der 8. Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz, 1967, vorgetragen und in Form einer kurzen Mitteilung veröffentlicht (Müller-Oerlinghausen et al., 1967).

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Müller-Oerlinghausen, B. Über die gestörte Glucuronidsynthese in der Leber diabetischer Ratten. Naunyn-Schmiedebergs Arch. Pharmak. 265, 372–382 (1970). https://doi.org/10.1007/BF00997195

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  • DOI: https://doi.org/10.1007/BF00997195

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