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Molecular Biology Reports

, Volume 19, Issue 2, pp 93–97 | Cite as

PKA controls a level of topoisomerase I mRNA in mouse L5178Y lymphoma cells treated with db-cAMP

  • R. M. Czerwinski
  • K. Nieznanski
  • B. Sochanowicz
  • K. Staron
Research Articles

Abstract

The level of topoisomerase I mRNA was measured in cells of two mouse lymphoma (LY) sublines treated with db-cAMP. A transient increase of the level was observed to be of about 60% of the basic level and to have maximum after the 3 h treatment of LY-S cells. The increase in LY-R subline was two-fold lower. The activity of PKA in a cytosol fraction of LY-S cells was 1.75 times higher than that in LY-R cells. The activity of PKA in membranes and nuclear fraction did not differ significantly in both cell types. When the activity of PKA in LY-S cells was inhibited with H8, no increase of the level of topoisomerase I mRNA was observed upon db-cAMP treatment of cells. We suggest that the activity of PKA in the cytosol controls the expression of topoisomerase I gene in LY cells at high concentration of cAMP.

Key words

topoisomerase 1 mRNA db-cAMP PKA activity mouse L5178Y lymphoma sublines 

Abbreviations

db-cAMP

dibutyryl-cAMP

H8

N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide

LY

mouse L5178Y lymphoma

PKA

protein kinase A

topo I

topoisomerase I

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • R. M. Czerwinski
    • 1
  • K. Nieznanski
    • 1
  • B. Sochanowicz
    • 2
  • K. Staron
    • 1
  1. 1.Institute of BiochemistryWarsaw UniversityWarsawPoland
  2. 2.Institute of Nuclear Chemistry and TechnologyWarsawPoland

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