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Die Wirkung von Furosemid und Etacrynsäure auf die Kohlenhydrat- und Fettsäureaufnahme und auf den Glykogengehalt der Hundeniere in vivo

Effects of furosemide and ethacrynic acid on renal uptake of carbohydrates and free fatty acids and on renal tissue concentration of glykogen in dogs

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Summary

The following parameters have been determined in anesthetized dogs: Urine volume (UV), GFR, renal blood flow (RBF), sodium excretion (ENa), tubular sodium reabsorption (TNa), the arterial and renal venous concentration,a- v difference and renal net uptake of glucose, lactate, pyruvate, and nonesterified fatty acids (FFA). After control periods, 5 mg/kg furosemide or ethacrynic acid have been injected intravenously. Tissue concentrations of glycogen in cortex and medulla have been compared before and after administration of the two diuretics. Both, furosemide and ethacrynic acid had comparable effects on UV, GFR, ENa and TNa at the doses given. The mean increase of RBF was 34% after ethacrynic acid and 23% after furosemide. The net uptake of the substrates in 68 control measurements in 17 dogs averaged 60.0±6.2 for lactate, 5.0±0.8 for pyruvate and 15.0±2.1 for FFA (μmol/min · 100 g kidney weight). The correlation of arterial load and net uptake was significant for lactate and FFA. In- and outflow of glucose were not significantly different. There was no net uptake of glucose even after a 4–5 fold increase of the arterial concentration by an i.v. infusion of glucose.

Neither furosemide nor ethacrynic acid had an effect on renal glucose balance. Both blocked the renal uptake of FFA completely. After furosemide the uptake of lactate and pyruvate averaged only about one half of the respective controls whereas there was a net release of both substrates after ethacrynic acid.

The glycogen concentration of the renal cortex amounted to 0.48 mmol glucose/kg wet weight in renal cortex and to 1.40 in medulla. Both diuretics increased tissue glycogen concentration by 20–25%.

One can conclude that both, furosemide and ethacrynic acid, inhibit the oxidative metabolism in renal cortex whereas anaerobic glycolysis is less or not affected. The results are discussed with regard to the constant oxygen consumption which has been observed in previous experiments.

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Authors and Affiliations

  1. Abteilung Pharmakologie der Medizinischen Fakultät der TH Aachen, Deutschland

    G. Fülgraff, J. Greven, H. Holzhüter, H. Nünemann, H. Osswald & D. Sudhoff

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  1. G. Fülgraff
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  2. J. Greven
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  3. H. Holzhüter
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  4. H. Nünemann
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  5. H. Osswald
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  6. D. Sudhoff
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Auszugsweise vorgetragen auf der 11. Frfihjahrstagung der Deutsehen Pharmakologischen Gesellschaft veto 15.-18.3.1970 in Mainz und auf der 37. Tagung der Deutschen Physiologischen Gesellschaft veto 10.-11.4. 1970 in Erlangen.

Mit Unterstiitzung durch die Deutsche Yorschungsgemeinschaft.

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Fülgraff, G., Greven, J., Holzhüter, H. et al. Die Wirkung von Furosemid und Etacrynsäure auf die Kohlenhydrat- und Fettsäureaufnahme und auf den Glykogengehalt der Hundeniere in vivo. Naunyn-Schmiedebergs Arch. Pharmak. 270, 117–135 (1971). https://doi.org/10.1007/BF00997083

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  • DOI: https://doi.org/10.1007/BF00997083

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