Naunyn-Schmiedebergs Archiv für Pharmakologie

, Volume 270, Issue 3, pp 262–273 | Cite as

Effects of staphylococcal alpha toxin on ion and water transports in isolated frog skin and bladder

  • O. Kadlec
  • R. Čapek


The action of staphylococcal alpha toxin (ST) on potential difference (PD) and short-circuit current (SCC) of the isolated frog skin was studied. Ringer solution bathed the corial side and 20 mM NaCl bathed the epidermal side. PD and SCC decreased to about half after the administration of ST to the corial side of the skin. Later, SCC raised considerably. The replacement of 20 mM NaCl by KCl on the epidermal side of the ST-pretreated skin did not cause any substantial decrease of PD, while in the untreated skin the same replacement caused a sharp drop of PD. No secondary increase of SCC was observed after ST administration when the Ringer solution bathing the corial side of the skin contained 1/2 S04 instead Cl. In contrast to the normal skin, dilution from 120 to 2 mM of the NaCl solution on the epidermal side led to a PD increase in the toxin-treated skin.

Na+ fluxes across the skin in both directions were measured by means of radioisotopes. The direction of net flux of Na+ was reversed after treatment with the toxin. The results demonstrated two phases of ST action. Na+ transport is damaged in the first phase; in the second phase an outflux of Cl is induced.

The changes of water permeability of the frog urinary bladder were determined by weighing bags formed from the bladders. The addition of the toxin to the medium bathing the serosal side resulted in increased weight losses. The transport of water was increased.


Staphylococcal Alpha Toxin Frog Skin and Bladder Na+, Cl and Water Transports 


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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • O. Kadlec
    • 1
  • R. Čapek
    • 1
    • 2
  1. 1.Institute of PharmacologyCzechoslovak Academy of SciencesPragueCzechoslovakia
  2. 2.Department of Pharmacology and TherapeuticsMcGill UniversityMontrealCanada

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