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Metabolic Brain Disease

, Volume 8, Issue 2, pp 115–124 | Cite as

Metabolic changes in axotomized fetal and early postnatal hamster facial motoneurons: An autoradiographic study

  • Pamela Clark
  • Kathryn J. Jones
  • Arthur LaVelle
Original Contribution

Abstract

The developing facial neurons of a series of hamsters ranging in age from the 14-day fetus to the 9 day postnatal were axotomized. Postoperative times were graded for each age so that the retrograde response could be observed before any significant amount of cell degeneration or death occurred. The incorporation of tritiated uridine was followed by the autoradiographic procedure. Although grain counts, relative to control values, were significantly reduced only in the axotomized fetus and at 24 hours postoperatively in 4-day postnatal animals, there was also a repression of isotopic incorporation in all the other axotomized animals. These results support data obtained from previous work with the hamsters which indicate that it is not until after the nerve cell nucleolus reaches full cytomorphic maturity (between 15 and 20 days postnatal age in hamster facial neurons) that the axotomized neurons respond with significantly increased incorporation levels of isotope over that of control neurons.

Key words

facial neurons peripheral motoneurons, axotomy regeneration development RNA synthesis 

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References

  1. Busch, H. and Smetana, K. (1970). The Nucleolus, Academic Press, New York.Google Scholar
  2. Clark, P., Jones, K.J. and LaVelle, A. (1990). Ultrastructural and morphometric analysis of nucleolar and nuclear changes during the early growth period in hamster facial neurons.J. Comp. Neurol.,302: 749–760.Google Scholar
  3. Clark, P., Jones, K.J. and LaVelle, A. (1992). Alterations in nuclear envelope investigations in axotomized fetal and early postnatal hamster facial neurons.Dev. Brain Res. 68: 1–8.Google Scholar
  4. Fischer, D., Weisenberger, D. and Scheer, U. (1991). Review: assigning functions to nucleolar structures.Chromosoma 101: 133–140.Google Scholar
  5. Hadjiolov, A.A. (1985). The Nucleolus and Ribosome Biogenesis, Cell Biology Monographs, Vol. 12, Springer-Verlag, New York.Google Scholar
  6. Jones, K.J. and LaVelle, A. (1985). Changes in nuclear envelope invaginations in axotomized immature and mature hamster facial motoneurons.Dev. Brain Res. 21: 241–249.Google Scholar
  7. Jones, K.J. and LaVelle, A. (1986a). Differential effects of axotomy on immature and mature hamster facial neurons: a time course study of initial nucleolar and nuclear changes.J. Neurocytol. 15: 197–206.Google Scholar
  8. Jones, K.J. and LaVelle, A. (1986b). Ultrastructural changes in the nucleoplasm of hamster facial neurons during a postnatal maturation period.Brain Res. 377: 119–126.Google Scholar
  9. Jones, K.J. and LaVelle, A. (1987). Differential effects of axotomy on immature and mature hamster facial neurons: a tritiated-uridine autoradiographic study.Metab. Brain Dis. 2: 259–269.Google Scholar
  10. Jordan, E.G. and Cullis, C.A. (1982). The Nucleolus, Cambridge University Press, New York.Google Scholar
  11. Kinderman, N.B. and LaVelle, A. (1976). Ultrastructural changes in the developing nucleolus following axotomy.Brain Res. 108: 237–247.Google Scholar
  12. LaVelle, A. and LaVelle, F.W. (1959). Neuronal reaction to injury during development: severance of the facial nerve in utero.Exp. Neurol. 1: 82–95.Google Scholar
  13. LaVelle, A. and LaVelle, F.W. (1958). Neuronal swelling and chromatolysis as influenced by the state of cell development.Am. J. Anat. 102: 219–241.Google Scholar
  14. LaVelle, A. and LaVelle, F.W. (1975a). Changes in a neuronal intranucleolar body in hamster facial neurons following axotomy.Anat. Rec. 181: 406.Google Scholar
  15. LaVelle, A. and LaVelle, F.W. (1975b). Changes in an intranucleolar body in hamster facial motor neurons during development and aging.Dev. Brain Res. 10: 171–175.Google Scholar
  16. McLoon, L.K. and LaVelle, A. (1981a). Tritiated leucine incorporation in the developing hamster facial nucleus with injury; a liquid scintillation study.Dev. Brain Res. 1: 237–248.Google Scholar
  17. McLoon, L.K. and LaVelle, A. (1981b). Long-term effects of regeneration and prevention of regeneration on nucleolar morphology after facial nerve injury during development.Exp. Neurol. 73: 762–773.Google Scholar
  18. McLoon, L.K. and LaVelle, A. (1981c). Tritiated leucine incorporation in the developing hamster facial neurons with injury: an autoradiographic study.Exp. Neurol. 74: 573–586.Google Scholar
  19. Sokal, R.R. and Rohlf, F.J. (1981). Biometry, W.H. Freeman and Company, San Francisco.Google Scholar
  20. Watson, W.E. (1968). Observations on the nucleolar and total cell body nucleic acid of injured nerve cells.J. Physiol. (London)196: 655–676.Google Scholar

Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • Pamela Clark
    • 1
  • Kathryn J. Jones
    • 1
  • Arthur LaVelle
    • 1
  1. 1.Department of Anatomy and Cell BiologyUniversity of Illinois at ChicagoChicago

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