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Aktuelle Gesichtspunkte zur Auswertung der Bestimmung von Faktor VIII-Aktivität, Faktor VIII-assoziiertem Protein und Faktor VIII-neutralisierendem Antikörper

Current aspects on evaluation of assays of factor-VIII activity, factor-VIII associated protein and factor-VIII neutralizing antibody

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Summary

In evaluating factor-VIII activity it should be noted that regarding the remaining activity of deficient plasma a linear reference curve is achieved. In the standard population factor-VIII activity and factor-VIII associated protein are distributed approximately lognormally. Due to this distribution certain results have been gained for the optimal choice of localisation and dispersion measures. It is assumed that the proportions of neutralized factor-VIII activity in plasma are distributed according to Poisson. The applicability of the Poisson distribution was also proved for the free factor-VIII activity portion. Due to the Poisson distribution the antibody unit is clearly defined, thus eliminating a further discussion on the establishment of an arbitrary standardized antibody unit.

Zusammenfassung

Bei der Bestimmung der Faktor VIII-Aktivität sollte beachtet werden, daß durch Berücksichtigung der Restaktivität des Mangelplasmas eine Linearisierung der Bezugskurve erreicht wird. Faktor VIII-Aktivität und Faktor VIII-assoziiertes Protein sind in der Normalpopulation annähernd lognormal verteilt. Aus dieser Verteilung ergeben sich für die Wahl der besten Lokalisations- und Dispersionsmaße Konsequenzen. Es wird angenommen, daß die Anteile der neutralisierten Faktor VIII-Aktivität im Plasma poissonverteilt sind. Für den Anteil der freien Faktor VIII-Aktivität konnte die Anwendbarkeit der Poisson-Verteilung nachgewiesen werden. Durch die Poisson-Verteilung ist die Antikörpereinheit eindeutig definiert. Hierdurch würde sich die weitere Diskussion über die Festsetzung einer arbiträren einheitlichen Antikörpereinheit erübrigen.

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  1. Abt. Innere Medizin m. S. Hämatoiogie und Onkologie, Klinikum Charlottenburg der Freien Universität Berlin, Spandauer Damm 130, D-1000, Berlin 19

    Günter Hintz

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Hintz, G. Aktuelle Gesichtspunkte zur Auswertung der Bestimmung von Faktor VIII-Aktivität, Faktor VIII-assoziiertem Protein und Faktor VIII-neutralisierendem Antikörper. Blut 36, 95–107 (1978). https://doi.org/10.1007/BF00996836

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