Zusammenfassung
Ein mit menschlicher Plazenta konditioniertes Medium (HPCM) stimuliert in vitro die Bildung von Kolonien durch menschliche granulopoetisch determinierte Stammzellen (CFU-C). In der vorliegenden Arbeit wird diese Koloniebildung untersucht. Die Mehrzahl der ausgetesteten HPCM-Präparationen stimuliert die Koloniebildung im selben Maße wie Leukozyten-Unterschichten. Die Dosis-Wirkungs-Kurve von HPCM weist ein breites Plateau auf. Zwischen der Zahl der Zellen im Kulturansatz und der Zahl der gebildeten Kolonien besteht eine lineare Korrelation. Die optimale Kulturdauer liegt zwischen 9 und 11 Tagen. Die Kolonien sind groß und zumeist dicht. Die Zugabe von reifen Granulozyten zur Kultur beeinflußt, optimale Kulturbedingungen vorausgesetzt, die Koloniebildung nicht. Diese Befunde weisen HPCM als eine geeignete Quelle koloniestimulierender Aktivität für die Routinebestimmung menschlicher CFU-C aus. Durch die Konstanz der Kulturbedingungen erscheint HPCM besonders geeignet für Verlaufsbeobachtungen menschlicher CFU-C.
Summary
Human placenta-conditioned medium (HPCM) has been reported to stimulate colony formation by human granulopoietic stem cells (CFU-C) in vitro. The present work was performed to further characterize this colony formation. The majority of HPCM batches tested stimulated colony growth equivalent to recombined human leukocyte feeder layers with optimal cellular composition. A broad plateau of the dose-response curve of HPCM was found. A linear correlation exists between the number of marrow cells plated and the number of colonies grown. Optimal duration of culture is between 9 and 11 days. Colonies are large and tend to be compact. Admixture of mature granulocytes does not affect the colony growth pattern under optimal culture conditions. These data document that HPCM is a suitable source of colony-stimulating activity for the routine assay of human CFU-C. Due to the constant colony stimulation, HPCM appears particularly valuable for longitudinal studies of human CFU-C.
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This work was supported by the Deutsche Forschungsgemeinschaft, SFB 112
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Lohrmann, H.P., Hansi, W. & Heimpel, H. Human placenta-conditioned medium for stimulation of human granulopoietic precursor cell (CFU-C) colony growth in vitro. Blut 36, 81–88 (1978). https://doi.org/10.1007/BF00996834
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DOI: https://doi.org/10.1007/BF00996834