, Volume 36, Issue 3, pp 135–144 | Cite as

Gel electrophoresis of the human erythrocyte membrane proteins: Aberrant patterns in hematological and non-hematological diseases

  • Volker Anselstetter
Leading Article


Public Health Membrane Protein Erythrocyte Membrane Human Erythrocyte Human Erythrocyte Membrane 
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  1. 1.
    Allen, D.W., Cadman, S., McCann, S.R., Finkel, B.: Increased membrane binding of erythrocyte catalase in hereditary spherocytosis and in metabolically stressed normal cells. Blood49, 113 (1977)Google Scholar
  2. 2.
    Ames, G. F., Nikaido, K.: Two-dimensional gel electrophoresis of membrane proteins. Biochemistry15, 616 (1976)Google Scholar
  3. 3.
    Anselstetter, V.: Entwicklung eines Verfahrens der zweidimensionalen Polyacrylamid-Gel-elektrophorese der Proteine und Glykoproteine der Zytoplasmamembran, erarbeitet am Erythrozyten des Menschen. M.D. Thesis, University of Erlangen-Nürnberg (1974)Google Scholar
  4. 4.
    Anselstetter, V., Horstmann, H.J.: Two-dimensional polyacrylamide-gel electrophoresis of the proteins and glycoproteins of the human erythrocyte membrane. Eur. J. Biochem.56, 259 (1975)Google Scholar
  5. 5.
    Anselstctter, V., Horstmann, H.J., Heimpel, H.: Congenital dyserythropoietic anaemia, types I and II: Aberrant pattern of erythrocyte membrane proteins in CDA II, as revealed by two-dimensional polyacrylamide gel electrophoresis. Brit. J. Haematol.35, 209 (1977)Google Scholar
  6. 6.
    Anselstetter, V.: Equipment for the separation of nanogram amounts of peptides and proteins by various techniques of one- and two-dimensional polyacrylamide-gel electrophoresis. Hoppe-Seylers Z. Physiol. Chem.358, 1174 (1977)Google Scholar
  7. 7.
    Atlas, S., Shapiro, B., Green, J.W.: Surface properties of erythrocytes: Normal, paroxysmal nocturnal hemoglobinuria and glutathione-treated cells. Biochim. Biophys. Acta323, 194 (1973)Google Scholar
  8. 8.
    Bhakdi, S., Knüfermann, H., Wallach, D.F.H.: Two-dimensional separation of erythrocyte membrane proteins. Biochim. Biophys. Acta394, 550 (1975)Google Scholar
  9. 9.
    Bienzle, U., Bhadki, S., Knüfermann, H., Niethammer, D., Kleihauer, E.: Abnormality of erythrocyte membrane protein in a case of congenital stomatocytosis. Klinische Wochenschrift55, 569 (1977)Google Scholar
  10. 10.
    Birchmeier, W., Singer, S.J.: On the mechanism of ATP-induced shape changes in human erythrocyte membranes. II. The role of ATP. J. Cell. Biol.73, 647 (1977)Google Scholar
  11. 11.
    Bjerrum, O.J.: Immunochemical investigation of membrane proteins. A methodological survey with emphasis placed on immunoprecipitation in gels. Biochim. Biophys. Acta472, 135 (1977)Google Scholar
  12. 12.
    Boivin, P., Galand, C.: Protéines de la membrane érythrocytaire. I. étude électrophorétique des protéines solubilisées des membranes d'érythrocytes humains normaux et pathologiques. Nouv. Rev. FranÇ. d'Hématol.14, 355 (1974)Google Scholar
  13. 13.
    Booth, A.G.: A novel system for the two-dimensional electrophoresis of membrane proteins. Biochem. J.163, 165 (1977)Google Scholar
  14. 14.
    Bretscher, M.S.: A major protein which spans the human erythrocyte membrane. J. Mol. Biol.59, 351 (1971)Google Scholar
  15. 15.
    Clark, M.R., Shohet, S.B.: Hybrid erythrocytes for membrane studies in sickle cell disease. Blood47, 121 (1976)Google Scholar
  16. 16.
    Cleveland, D.W., Fischer, S.G., Kirschner, M.W., Laemmli, U.K.: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J. Biol. Chem.252, 1102 (1977)Google Scholar
  17. 17.
    Conrad, M.J., Penniston, J.T.: Resolution of erythrocyte membrane proteins by two-dimensional electrophoresis. J. Biol. Chem.251, 253 (1976)Google Scholar
  18. 18.
    Dahr, W., Uhlenbruck, G., Wagstaff, W., Leikola, J.: Studies on the membrane glycoprotein defect of En(a-) erythrocytes. II. Mn antigenic properties of En(a−) erythrocytes. J. Immuno-genet.3, 383 (1976)Google Scholar
  19. 19.
    Dahr, W., Uhlenbruck, G., Knott, H.: The defect of MK erythrocytes as revealed by sodium dodecyl-sulphate-polyacrylamide gel electrophoresis. J. Immunogenet.4, 191 (1977)Google Scholar
  20. 20.
    Dalmasso, A.P., Pizzimenti, M.C., Vacs, E., Diaz, A.: Abnormal solubilization by Triton X-100 of erythrocyte membranes from patients with paroxysmal nocturnal hemoglobinuria. Proc. Soc. Exp. Biol. Med.147, 273 (1974)Google Scholar
  21. 21.
    Durocher, J.R., Conrad, M.E.: Erythrocyte membrane proteins in sickle cell anemia. Proc. Soc. Exp. Biol. Med.146, 373 (1974)Google Scholar
  22. 22.
    Ehrlich, P.: In: Paul Ehrlich Gesammelte Arbeiten. Himmelwelt, F. (ed.). Berlin, Göttingen, Heidelberg: Springer 1957Google Scholar
  23. 23.
    Fairbanks, G., Steck, T.L., Wallach, D.F.H.: Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry10, 2606 (1971)Google Scholar
  24. 24.
    Furthmayr, H., Marchesi, V.T.: Subunit structure of human erythrocyte glycophorin A. Biochemistry15, 1137 (1976)Google Scholar
  25. 25.
    Gahmberg, C.G.: External labeling of human erythrocyte glycoproteins. Studies with galactose oxidase and fluorography. J. Biol. Chem.251, 510 (1976)Google Scholar
  26. 26.
    Gockerman, J.P., Durocher, J.R., Conrad, M.E.: The abnormal surface characteristics of the red blood cell membrane in congenital dyserythropoietic anemia type II (HEMPAS). Brit. J. Haematol.30, 383 (1975)Google Scholar
  27. 27.
    Gomperts, E.D., Metz, J., Zail, S.S.: A red cell membrane protein abnormality in hereditary spherocytosis. Brit. J. Haematol.23, 363 (1972)Google Scholar
  28. 28.
    Gomperts, E.D., Cayannis, F., Metz, J., Zail, S.S.: A red cell membrane protein abnormality in hereditary elliptocytosis. Brit. J. Haematol.25, 415 (1973)Google Scholar
  29. 29.
    Hayashi, S., Koomoto, R., Yano, A., Ishigami, S., Tsujino, G., Saeki, S., Tanaka, T.: Abnormality in a specific protein of the erythrocyte membrane in hereditary spherocytosis. Bio-chem. Biophys. Res. Commun.57, 1038 (1974)Google Scholar
  30. 30.
    Helenius, A., Simons, K.: Solubilization of membranes by detergents. Biochim. Biophys. Acta415, 29 (1975)Google Scholar
  31. 31.
    Helenius, A., Simons, K.: Charge shift electrophoresis: Simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution. Proc. Nat. Acad. Sci. USA74, 529 (1977)Google Scholar
  32. 32.
    Hiller, G., Weber, K.: Spectrin is absent in various tissue culture cells. Nature266, 181 (1977)Google Scholar
  33. 33.
    Iida, H., Hasegawa, I., Nozawa, Y.: Biochemical studies on abnormal erythrocyte membranes. Protein abnormality of erythrocyte membrane in biliary obstruction. Biochim. Biophys. Acta443, 394 (1976)Google Scholar
  34. 34.
    Jackson, P., Whittaker, M.: Evidence for an abnormality in the erythrocyte membranes of patients having paroxysmal nocturnal haemoglobinuria and aplastic anemia. Clin. Chim. Acta41, 299 (1972)Google Scholar
  35. 35.
    Kirkpatrick, F., Gordesky, S.E., Marinetti, G.V.: Differential solubilization of proteins, phospholipids, and cholesterol of erythrocyte membranes by detergents. Biochim. Biophys. Acta345, 154 (1974)Google Scholar
  36. 36.
    Kitao, T., Kawamura, H., Hattori, K., Takeshita, M.: Hemolytic anemia with abnormal membrane protein. Clin. Chim. Acta47, 319 (1973)Google Scholar
  37. 37.
    Kitao, T., Hattori, K., Takeshita, M.: Electrophoretic analysis of the major proteins of the human red cell membrane. Clin. Chim. Acta49, 353 (1973)Google Scholar
  38. 38.
    Kotsifopoulos, P.N.: Red cell membrane proteins abnormality in paroxysmal nocturnal hemo-globinuria and in in vitro induced PNH-like erythrocytes. Acta haemat.56, 328 (1976)Google Scholar
  39. 39.
    Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacterio-phage T 4. Nature227, 680 (1970)Google Scholar
  40. 40.
    Marchesi, V.T., Furthmayr, H., Tomita, M.: The red cell membrane. Ann. Rev. Biochem.45, 667 (1976)Google Scholar
  41. 41.
    Nickson, J.K., Jones, M.N.: Reconstitution of the monosaccharide-transport system of the human erythrocyte membrane. Biochem. Soc. Trans.5, 147 (1977)Google Scholar
  42. 42.
    Nozawa, Y., Noguchi, T., Iida, H., Fukushima, H., Sekiya, T., Ito, Y.: Erythrocyte membrane of hereditary spherocytosis: Alteration in surface ultrastructure and membrane proteins, as inferred by scanning electron microscopy and SDS-disc gel electrophoresis. Clin. Chim. Acta55, 81 (1974)Google Scholar
  43. 43.
    Pinder, J.C., Tidmarsh, S., Gratzer, W.B.: Integrity of polypeptide chains of spectrin from human erythrocytes. Arch. Biochem. Biophys.172, 654 (1976)Google Scholar
  44. 44.
    Porter, W.H., Preston, J.L.: Retention of trypsin and chymotrypsin proteolytic activity in sodium dodecyl sulfate solutions. Anal. Biochem.66, 69 (1975)Google Scholar
  45. 45.
    Riggs, M.G., Ingram, V.M.: Differences in erythrocyte membrane proteins and glycoproteins in sickle cell disease. Biochem. Biophys. Res. Commun.74, 191 (1977)Google Scholar
  46. 46.
    Righetti, P.G., Perrella, M., Zanella, A., Sirchia, G.: The membrane abnormality of the red cell in paroxysmal nocturnal haemoglobinuria. Nature245, 273 (1973)Google Scholar
  47. 47.
    Schrier, S.L.: Human erythrocyte membrane enzymes: Current status and clinical correlates. Blood50, 227 (1977)Google Scholar
  48. 48.
    Schwoch, G., Passow, H.: Preparation and properties of human erythrocyte ghosts. Mol. Cell. Biochem.2, 197 (1973)Google Scholar
  49. 49.
    Sheetz, M.P., Singer, S.J.: On the mechanism of ATP-induced shape changes in human erythrocyte membranes. I. The role of the spectrin complex. J. Cell Biol.73, 638 (1977)Google Scholar
  50. 50.
    Singer, S.J.: The proteins of membranes. J. Colloid Interface Sci.58, 452 (1977)Google Scholar
  51. 51.
    Singer, S.J., Nicolson, G.L.: The fluid mosaic model of the structure of cell membranes. Science175, 720 (1972)Google Scholar
  52. 52.
    Stierstorfer, S., Horstmann, H.J., Anselstetter, V.: A quantitative assay of proteins in suspensions of erythrocyte membranes. Hoppe-Seylers Z. Physiol. Chem.358, 1287 (1977)Google Scholar
  53. 53.
    Strapazon, E., Steck, T.L.: Interaction of the aldolase and the membrane of human ery-throcytes. Biochemistry16, 2966 (1977)Google Scholar
  54. 54.
    Tanford, C, Reynolds, J.A.: Characterization of membrane proteins in detergent solutions. Biochim. Biophys. Acta457, 133 (1976)Google Scholar
  55. 55.
    Tanner, M.J.A., Anstee, D.J.: The membrane change in En(a-) human erythrocytes. Absence of the major erythrocyte sialoglycoprotein. Biochem. J.153, 271 (1976)Google Scholar
  56. 56.
    Tilney, L.G., Detmers, P.: Actin in erythrocyte ghosts and its association with spectrin. Evidence for a nonfilamentous form of these two molecules in situ. J. Cell Biol.66, 508 (1975)Google Scholar
  57. 57.
    Valentine, W.N.: The molecular lesion of hereditary spherocytosis (HS): A continuing enigma. Blood49, 241 (1977)Google Scholar
  58. 58.
    Verkleij, A.J., Zwaal, R.F.A., Roelofson, B., Comfurius, P., Kastelijn, D., van Deenen, L.L.M.: The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy. Biochim. Biophys. Acta323, 178 (1973)Google Scholar
  59. 59.
    Wolosin, J.M., Ginsburg, H., Cabantchik, Z.I.: Functional characterization of anion transport system isolated from human erythrocyte membranes. J. Biol. Chem.252, 2419 (1977)Google Scholar
  60. 60.
    Yu, J., Steck, T.L.: Association of band 3, the predominant polypeptide of the human erythrocyte membrane. J. Biol. Chem.250, 9176 (1975)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Volker Anselstetter
    • 1
  1. 1.Institut für Physiologische Chemie der UniversitÄt Erlangen-NürnbergErlangenFederal Republic of Germany

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