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Isoenzym-Muster der sauren Phosphatasen von Epstein-Barr-Virus-DNS enthaltenden, permanent wachsenden lymphoiden Zell-Linien

Isoenzyme pattern of acid phosphatase in Epstein-Barr-virus-DNA positive permanent growing lymphoid cell lines

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Summary

The expression of acid phosphatases is cytochemically one of the most important features in permanent growing B-cell-lines. In few cell lines acid phosphatase is resistant against tartrate.

Tartrate resistant isoenzyme 5 with components a und b can be demonstrated in monocytes, lymphocytes, chronic lymphatic leucemic cells and especially in hairy cells as well as in cell lines derived from a healthy donor.

Fractionation of acid phosphatase by gelelectrophoresis in separated lymphocytes demonstrates especially isoenzyme 3, in separated macrophages isoenzyme 4. Isoenzyme 4 could not be detected in several cell lines. It is therefore concluded that these cell lines are probably derived from lymphocytic precursors. Cell lines with isoenzyme 4 may be the result of a facultative hybridisation between lymphocytes and monocytes.

Profiles of acid phosphatases in virus-negative cell lines (Ramos, BJAB) were not significantly altered by conversion with EBV.

Zusammenfassung

Die Expression der sauren Phosphatasen sind das stärkste zytochemische Merkmal lymphoider Zellinien. In einigen Zellen erscheint die saure Phosphatase zytochemisch resistent gegen Tartrat.

Die tartratresistenten Isoenzyme 5 mit den Komponenten a und b lassen sich sowohl bei Fraktionierung der sauren Phosphatasen aus Monozyten, Lymphozyten, chronisch lymphatischen Leukämiezellen und besonders aus Haarzellen als auch in unterschiedlicher Ausprägung in Zellinien von einem gesunden Spender darstellen.

Die gelelektrophoretische Fraktionierung der sauren Phosphatasen in angereicherten Lymphozyten zeigt vorwiegend das Isoenzym 3, in angereicherten Makrophagen das Isoenzym 4. In einigen Zellinien ist das Isoenzym 4 nicht nachzuweisen. Es wird daraus geschlossen, daß diese Zellinien möglicherweise ausschließlich lymphozytären Ursprungs sind, während diejenigen mit Nachweis des Isoenzyms 4 vielleicht Produkte einer fakultativen Hybridisierung aus Lymphozyten und Monozyten sind.

Profile der sauren Phosphatasen in Virus-negativen Zellinien (Ramos, BJAB) werden durch Konversion mit EBV nicht eindeutig verändert.

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Literatur

  1. Axline, S.G.: Isoenzymes of acid phosphatase in normal and Calmette-Guerin Bacillus induced rabbit alveolar macrophages. J. Exp. Med.128, 1031–1048 (1968)

    Google Scholar 

  2. Fresen, K.O., zur Hausen, H.: Establishment of EBNA-expressing cell lines by infection of Epstein-Barr-Virus (EBV)-genome-negative human lymphoma cells with different EBV strains. Int. J. Cancer17, 161–166 (1976)

    Google Scholar 

  3. Fresen, K.O., Merkt, B., Bornkamm, G.W., zur Hausen, H.: Heterogeneity of Epstein-Barr Virus originating from P3HR-1 cells. I. Studies on EBNA Induction. Int. J. Cancer19, 317–323 (1977)

    Google Scholar 

  4. zur Hausen, H., Schulte-Holthausen, M.: Presence of EB-virus nucleic homology in a “virus-free“ line of Burkitt tumour cells. Nature227, 245–248 (1970)

    Google Scholar 

  5. zur Hausen, H., Diehl, V., Wolf, H., Schulte-Holthausen, M., Schneider, U.:Occurrence of Epstein-Barr Virus genomes in human lymphoblastoid cell lines. Nature N.B.237, 189–190 (1972)

    Google Scholar 

  6. Heckner, F.: Färbechemie. In: Handbuch der gesamten Hämatologie (Hrsg. L.A. Heilmeyer und Hittmair), Bd. II/2, S. 79. München: Urban u. Schwarzenberg 1960.

    Google Scholar 

  7. v. Heyden, H.W., v. Heyden, D.: Establishment of lymphocytoid cell lines in relation to number of leucocytes, surface area of monolayer formation, and feeding procedures. In Vitro8, 309–315 (1973)

    Google Scholar 

  8. v. Heyden, H.W., v. Heyden, D.: Cytological characteristics of normal human peripheral blood cells in culture before establishment into lymphocytoid cell lines. Cell Tissue Kinet.6, 271–280 (1973)

    Google Scholar 

  9. v. Heyden, H.W., Waller, H.D., Pape, G.R., Benöhr, H. Chr., Braun, H.J., Wilms, K., Rieber, E.P., Riethmüller, G.: Haarzell-Leukämie. Klinik, Zytochemie, Phagozytosefähigkeit von Haarzellen, Etablierung permanent wachsender Zellinien. Dtch. Med. Wschr.101, 3–8 (1976)

    Google Scholar 

  10. Jondal, M., Klein, G.: Surface markers on human B-and T-lymphocytes. II. Presence of Epstein-Barr Virus receptors on B-lymphocytes. J. Exp. Med.138, 1365–1378 (1973)

    Google Scholar 

  11. Kaplow, L.S.: A histochemical procedure for localizing and evaluating leukocytes alkaline phosphatase activity in smears of blood and marrow. Blood10, 1023–1029 (1953)

    Google Scholar 

  12. Klein, G., Dombos, L., Gothoskar, B.: Sensitivity of Epstein-Barr virus (EBV) producer and nonproducer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer10, 44–57 (1972)

    Google Scholar 

  13. Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menezes, J., Nilsson, K., Sundström, Ch.: Continuous lymphoid cell lines with characteristics of B-cells (Bone marrow derived) lacking the Epstein-Barr Virus genome and derived from three human lymphomas. Proc. Nat. Acad. Sci.71, 3283–3286 (1974)

    Google Scholar 

  14. Klein, G., Giovanella, B., Westman, A., Stehlin, J.S., Mumford, D.: An EB-genoemnegative cell-line established from an American Burkitt lymphoma; receptor characteristics, EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology5, 319–334 (1975)

    Google Scholar 

  15. Leder, L.D.: Der Blutmonozyt. Berlin-Heidelberg-New York: Springer 1967

    Google Scholar 

  16. Li, C.Y., Yam, L.T., Lam, K.W.: Acid phosphatase in human leucocytes in normal and pathologic conditions. J. Histochem. Cytochem.18, 473–481 (1970)

    Google Scholar 

  17. Li, C.Y., Yam, L.T., Lam, K.W.: Studies of acid phosphatase isoenzymes in human leucocytes. Demonstration of isoenzyme cell specifity. J. Histochem. Cytochem.18, 901–910 (1970)

    Google Scholar 

  18. Löffler, H., Zytochemischer Nachweis von unspezifischen Esterasen in Ausstrichen. Klin. Wschr.39, 1220–1227 (1961)

    Google Scholar 

  19. Löffler, H., Berghoff, W.: Eine Methode zum Nachweis saurer Phosphatasen in Ausstrichen. Klin. Wschr.40, 363–364 (1962)

    Google Scholar 

  20. Maurer, H.R.: Disk Electrophoresis and related techniques of Polyacrylamide Gel Electrophoresis. Berlin-New York: W. de Gruyter 1971

    Google Scholar 

  21. Merker, H., Heilmeyer, L.: Die alkalische Phosphatase neutrophiler Leukozyten. Dtsch. Med. Wschr.85, 253–258 (1960)

    Google Scholar 

  22. Nilsson, K., Pontén, J.: Classification and biological nature of established human hematopoietic cell lines. Int. J. Cancer15, 321–341 (1975)

    Google Scholar 

  23. Pope, J.H., Scott, W., Moses, D.J.: Cell relationship in transformation of human leukocytes by Epstein Barr Virus. Int. J. Cancer14, 122–129 (1974)

    Google Scholar 

  24. Rieber, E.P., v. Heyden, H.W., Linke, R.P., Saal, J.G., Riethmüller, G., Waller, H.D.: Haarzelleukämie. Charakterisierung der leukämischen Zellen: Oberflächenimmunglobuline, Fc-Rezeptoren und Stimulation durch Mitogen. Klin. Wschr.54, 1011–1019 (1976)

    Google Scholar 

  25. Riethmüller, G., Saal, G.J., Rieber, E.P., Ehinger, H., Schnellen, B., Riethmüller, D.: Cellular immune response to melanoma tumor cells. Effect of BCG treatment cell mediated cytotoxicity measured with 3H Prolin test in vitro. Transplantation Proc.7, 495–498 (1975)

    Google Scholar 

  26. Saal, J.G., Rieber, E.P., Riethmüller, G.: Cell-mediated cytotoxicity for melanoma tumor cells. Detection by a 3H-Prolin release assay. Scand. J. immunol.5, 455–466 (1976)

    Google Scholar 

  27. Schneider, U., zur Hausen, H.: Epstein-Barr-Virus induced transformation of human leucocytes after cell fractionation. Int. J. Cancer15, 59–66 (1975)

    Google Scholar 

  28. Yam, L.T., Li, C.Y., Lam, K.W.: Tartrate resistant acid phosphatase isoenzyme in the reticuloendotheliosis. N. Engl. J. Med.284, 357–360 (1971)

    Google Scholar 

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Authors and Affiliations

  1. Medizinische Universitätsklinik Tübingen, Abteilung Innere Medizin II, Otfried-Müller-Straße, D-7400, Tübingen

    H. -W. v. Heyden, R. Weber & H. Stuckstedte

  2. Abteilung Experimentelle Chirurgie und Immunologie, Chirurgische Universitätsklinik Tübingen, D-7400, Tübingen

    J. -G. Saal

  3. Zentrum für Hygiene, Institut für Virologie, D-7800, Freiburg i. Brsg

    K. O. Fresen

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  1. H. -W. v. Heyden
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  2. R. Weber
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  3. H. Stuckstedte
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  4. J. -G. Saal
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  5. K. O. Fresen
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Diese Arbeit wurde von der Deutschen Forschungsgemeinschaft im Rahmen der Forschergruppe „Biochemische und immunologische Grundlagen der Leukämie und Tumortherapie“ unterstützt

Diese Veröffentlichung enthält wesentliche Ergebnisse der Promotionsarbeiten dieser Autoren.

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v. Heyden, H.W., Weber, R., Stuckstedte, H. et al. Isoenzym-Muster der sauren Phosphatasen von Epstein-Barr-Virus-DNS enthaltenden, permanent wachsenden lymphoiden Zell-Linien. Blut 35, 395–404 (1977). https://doi.org/10.1007/BF00996626

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  • DOI: https://doi.org/10.1007/BF00996626

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