Summary
Human transfer factor was prepared from normal donors with marked skin reactivity against common microbial antigens by dialysis of lymphocyte extracts versus tissue culture medium (TFmd). Several batches of TFmd were tested for their ability to specifically increase the DNA synthesis of unsensitized lymphocytes in vitro in the presence of the corresponding antigens (PPD, SKSD, Candida, Histoplasmin). No transfer of immunologic reactivity by TFmd was observed. There was, however, a significant unspecific inhibition by TFmd of lymphocyte cultures stimulated by antigens, PHA or allogeneic cells.
Zusammenfassung
Menschlicher Transferfaktor wurde von normalen Spendern mit ausgeprägten verzögerten Hautreaktionen gegen gewöhnliche mikrobielle Antigene durch Dialyse von Lymphozytenextrakt gegen Gewebekulturmedium hergestellt (TFmd). Verschiedene Präparationen von TFmd wurden auf ihre Fähigkeit untersucht, die DNA-Synthese von unsensibilisierten Lymphozyten in vitro in Anwesenheit der korrespondierenden Antigene (PPD, SKSD, Candida, Histoplasmin) spezifisch zu steigern. In keinem Fall konnte ein Transfer immunologischer Reaktivität durch TFmd nachgewiesen werden. Jedoch ließ sich häufig ein signifikanter, unspezifischer Hemmeffekt des TFmd in Lymphozytenkulturen feststellen, die mit Antigenen, PHA oder allogeneischen Zellen stimuliert wurden.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahern Th.: An assessment of the role of transfer factor in vitro and in vivo models of delayed hypersensitivity.Bebring Inst. Mitt. 57 (1975), 17.
Arala-Chaves M., Ramos M. T. F., Rosado R. & Branco P.: Transfer factor in vitro. Additional data concerning a new method.Int. Arch. Allergy 46, 612–618 (1974).
Ascher M. S., Schneider W. J., Valentine F. T. & Lawrence H. S.: In vitro properties of leukocyte dialyzates contantaining transfer factor.Proc. Natl. Acad. Sci. USA 71, 1178–1182 (1974).
Dressler D. & Potter H.: Transfer factor: Warning on uncertainty of results.Ann. Int. Med. 82, 279 (1975).
Dressler D. & Rosenfeld S.: On the chemical nature of transfer factor.Proc. Natl. Acad. Sci. USA 71, 4429–4434 (1974).
Dupont B. O., Ballow M., Hansen J. A., Quick C., Yunis E. & Good R. A.: Effect of transfer factor therapy on mixed lymphocyte culture reactivity.Proc. Natl. Acad. Sci 71, 867–871 (1974).
Hamblin A.: The effect of transfer factor on cultured lymphocytes.Behring Inst. Mitt. 57 (1975), 25.
Kirkpatrick C. H. & Gallin J. I.: Suppression of cellular immune responses following transfer factor: Report of a case.Cellular Immunology 15, 470–474 (1975).
Lawrence H. S.: Mediators of cellular immunity.Transplant. Proc. 5, 49–58 (1973).
Lawrence H. S.: Transfer factor. In: Mediators of cellular immunity (H. S. Lawrence and M. Landy, Editors) (Academic Press, London, pp. 143–175, 1969).
Reymond J.-F. & Grob P. J.: Transfer factor preparations: Some physicochemical and biological properties.Vox Sang. 29, (1975) 338.
Schröder I., Werner H., Lorenz U., Köhler H. & Jenssen H. L.: Zur Präparation und Charakterisierung von TransferFaktor.Z. Immun.-Forsch. 149 (1975), 365.
Valdimarsson H.: The influence of dialysable leukocyte extracts on immune systems.Bebring Inst. Mitt. 57 (1975) 11.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mueller-Eckhardt, C., Ritts, R.E. Inhibitory activity of medium-dialyzed transfer factor on lymphocyte blastogenesis. Blut 32, 353–360 (1976). https://doi.org/10.1007/BF00996475
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00996475