Abstract
We have examined the endogenous nuclease activity of the liver of intact, castrated and testosterone-treated mice of different ages. Both Mg2+- and Ca2+-dependent endogenous nuclease activities decline in old age. Withdrawal of the hormone increases nuclease activity in the immature and young. However, testosterone administration prevents the digestion of nuclei to different extents in all ages. These findings suggest a possible protective role of testosterone in the cleavage of liver chromatin by endogenous nucleases during the aging of mice.
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References
Simpson RT & Whitlock JP Jr (1976) Nucl. Acid. Res. 3: 117–127.
Hewish DR & Burgoyne LA (1973) Biochem. Biophys. Res. Commun. 52: 504–510.
Wyllie AH (1980) Nature 284: 555–556.
Kyprianou N & Issacs JT (1988) Endocrin. 20: 552–562.
Shalka M, Matyasova J & Geijova M (1976) FEBS Lett. 72: 241–247.
Sambrook J, Fritsch RF & Maniatis T (1989) Molecular Cloning — A Laboratory Manual. Cold Spring Harbour Laboratory, Cold Spring Harbour, New York.
Finch JT & Klug A (1976) Proc. Natn. Acad. Sci. 73: 1897–1901.
Ishida R, Akiyshi H & Takahasi T (1974) Biochem. Biophys. Res. Commun. 56: 703–714.
Yamamoto KR (1985) Annu. Rev. Genet. 19: 209–252.
Thakur MK (1995) Curr. Sci. 68: 806–812.
Roy AK (1973) Endocrin. 92: 957–960.
Paul J & Duerksen JD (1976) Biochem. Biophys. Res. Commun. 68: 97–105.
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Mukherjee, S., Asaithambi, A. & Thakur, M.K. Androgen treatment protects mouse liver chromatin from cleavage by endogenous nucleases during aging. Mol Biol Rep 22, 59–61 (1995). https://doi.org/10.1007/BF00996306
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DOI: https://doi.org/10.1007/BF00996306