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Blut

, Volume 34, Issue 4, pp 329–332 | Cite as

Cyclic 3′,5′-adenosine monophosphate level and adenylate cyclase activity in human blood platelets during storage in ACD solution

  • Evelyne Lammerant-Guillemare
  • Françoise Corcelle-Cerf
  • Jacques Lammerant
Kurze Mitteilung

Summary

The level of cyclic 3′,5′-adenosine monophosphate (cAMP) in human blood platelets and the activity of platelet adenylate cyclase in response to prostaglandin E1 stimulation do not change during two days storage at room temperature in ACD solution. However, the level of cyclic AMP is lower in platelets stored in ACD solution than in platelets from blood anticoagulated by ethylenediamine tetra-acetic acid.

Key words

Platelet storage cAMP prostaglandin E1 ACD EDTA 

Zusammenfassung

Die Konzentration von zyklischem AMP und die Aktivität der Adenylat-Zyklase nach Stimulation mit Prostaglandin E1 in menschlichen Thrombozyten ändert sich nicht, wenn diese zwei Tage bei Zimmertemperatur in ACD-Lösung gelagert werden. Die Konzentration von zyklischem AMP in derart gelagerten Blutplättchen ist niedriger als in Thrombozyten, die aus EDTA ungerinnbar gemachtem Blut gewonnen werden.

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References

  1. 1.
    Biochemistry and Pharmacology of Platelets,Ciba Foundation Symposium 35, new series (Elsevier, Excerpta Medica, North-Holland, Amsterdam, 1975).Google Scholar
  2. 2.
    Robison G. A., Arnold A. & Hartmann R. C.: Divergent effects of epinephrine and prostaglandin E1 on the level of cyclic AMP in human blood platelets.Pharmacol. Res. Commun. 1, 325 (1969).Google Scholar
  3. 3.
    Tovey K. C., Oldham K. G. & Whelan J. A. M.: A simple direct assay for cyclic AMP in plasma and other biological samples using an improved competitive protein binding technique.Clin. chim. Acta 56, 221 (1974).Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Evelyne Lammerant-Guillemare
    • 1
    • 2
  • Françoise Corcelle-Cerf
    • 1
    • 2
  • Jacques Lammerant
    • 1
  1. 1.Centre of Blood TransfusionRed Cross
  2. 2.Department of PhysiologySchool of MedicineNamur

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