Zusammenfassung
Die rheologischen Eigenschaften der Erythrozyten von 10 reifen Neugeborenen wurden mit einer Filtrationsmethode und Viskositätsmessungen an Erythrozytensuspensionen mit einem Hämatokrit von 80% untersucht. Die Erythrozyten der Neugeborenen waren schlechter verformbar als Erythrozyten gesunder Erwachsener. Die Rigidität der Erythrozyten beruht auf einem erhöhten Hämoglobingehalt der Erythrozytenmembranen. Die Verformbarkeit von Erythrozyten Erwachsener sank ab, sobald sich in den mit Acetylphenylhydrazin inkubierten Zellen Heinz-Körper gebildet hatten. In Erythrozyten Neugeborener bildeten sich unter gleichen Inkubationsbedingungen mehr Heinz-Körper. Diese Zellen waren noch rigider als die inkubierten Erythrozyten Erwachsener. Aus den in vitro gewonnenen Ergebnissen wird geschlossen, daß die rigiden Erythrozyten der Neugeborenen, besonders wenn sie Heinz-Körper enthalten, in vivo die venösen Sinus der Milz schlecht passieren können. Die rheologischen Untersuchungen erklären, warum bei Neugeborenen die Überlebenszeit der Erythrozyten verkürzt ist.
Summary
The rheological properties of erythrocytes of 10 full-term infants were studied. A method of filtration and measurements of viscosity of erythrocyte suspensions with a hematocrit of 80% were used. Erythrocytes of newborn infants were less flexible than erythrocytes of healthy adults. Rigidity of erythrocytes was due to an increased hemoglobin content of the erythrocyte membranes which was measured in isolated ghosts. After incubation of the cells with acetylphenylhydrazin Heinz-bodies were formed in the erythrocytes of adults. The flexibility of these cells decreased markedly. More Heinz-bodies were found in the erythrocytes of the newborn infants, when their cells were incubated under identical conditions. These erythrocytes became more rigid than the erythrocytes of adults. From these results we conclude that the more rigid erythrocytes of the newborn infants could not pass the splenic sinus, especially when they contain Heinz-bodies. The rheological properties of the erythrocytes of newborn infants explain the shortened life-span of red cells of newborn infants.
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Tillmann, W., Wagner, D. & Schröter, W. Verminderte Flexibilität der Erythrozyten von Neugeborenen. Blut 34, 281–288 (1977). https://doi.org/10.1007/BF00996204
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DOI: https://doi.org/10.1007/BF00996204