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Heterogeneity of human red cell membrane sialoglycoproteins

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Summary

Discontinuous sodium dodecylsulfate polyacrylamide gel electrophoresis (disc SDS-PAGE) followed by periodic acid/Schiff staining reveals the presence of six sialoglycoprotein bands in human red cell membranes or glycoprotein preparations therefrom. In agreement with previous investigations it is shown that PAS-1 and PAS-2 (mol. weight 37 000) are different forms of the same molecule (MN glycoprotein). Using separation of glycoproteins by the system ofWeber andOsborn and reelectrophoresis of gel slices by disc SDS-PAGE it is demonstrated that the minor component C (mol. weight 41 000) represents the dimeric form of PAS-3 (Ss glycoprotein). Band B corresponds to an aggregate of PAS-3 and PAS-2 and/or the trimer of PAS-3 with possible differences between extracted glycoproteins and those present in the membrane. The minor component D (mol. weight 35 000) is, as far as could be elucidated, not involved in aggregation phenomena. Some technical problems of glycoprotein fractionation by SDS-PAGE and the remarkable effect of phosphate buffers on the glycoprotein pattern are discussed.

Zusammenfassung

Durch diskontinuierliche Natrium-Dodecylsulfat Polyacrylamid Gel Elektrophorese (disc SDS-PAGE) und anschlie\ende PerjodsÄure/Schiff-FÄrbung lassen sich sechs Sialoglykoproteinbanden in den aus menschlichen Erythrozytenmembranen extrahierten Glykoproteinpreparationen feststellen. In übereinstimmung mit früheren Untersuchungen wird gezeigt, da\ die Banden PAS-1 und PAS-2 (Mol.-Gewicht 37 000) verschiedene Formen desselben Moleküls (MN Glykoprotein) darstellen. Durch Auftrennung von Glykoproteinen mit dem System vonWeber undOsborn und anschlie\ende Reelektrophorese von Gelstücken mit disc SDS-PAGE wird nachgewiesen, da\ die Komponente C (Mol.-Gewicht 41 000) das dimere PAS-3 (Ss Glykoprotein) darstellt. Die Bande B entspricht einem Aggregat aus PAS-2 und PAS-3 und/oder dem Trimer von PAS-3. Möglicherweise bestehen Differenzen zwischen extrahierten Glykoproteinen und solchen in der Membran. Die Komponente D (Mol.-Gewicht 35 000) ist, soweit sich klÄren lie\, nicht an AggregationsphÄnomenen beteiligt. Einige technische Probleme der Glykoproteinauftrennung mit SDS-PAGE und der bemerkenswerte Effekt von Phosphatpuffern auf das Glykoproteinmuster werden diskutiert.

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  1. Wolfgang Dahr

    Present address: Abteilung für Immunbiologie der Medizinischen UniversitÄtsklinik Köln, Deutschland

Authors and Affiliations

  1. Abteilung für Immunbiologie der Medizinischen UniversitÄtsklinik Köln, Deutschland

    Gerhard Uhlenbruck, Eveline Jan\en & Renate Schmalisch

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  1. Wolfgang Dahr
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  2. Gerhard Uhlenbruck
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  3. Eveline Jan\en
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  4. Renate Schmalisch
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Herrn Prof. Dr. Peter Dahr zum 70. Geburtstag gewidmet.

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Dahr, W., Uhlenbruck, G., Jan\en, E. et al. Heterogeneity of human red cell membrane sialoglycoproteins. Blut 32, 171–184 (1976). https://doi.org/10.1007/BF00995910

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