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Studies on the receptors of the MNSs blood group system

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Summary

Results with modified human red cell membrane sialoglycoproteins indicate that alkali-labile sialic acid and amino groups are parts of the erythrocyte receptor sites recognized by common rabbit and human anti-M and -N sera. The „N“ antigen, demonstrable in MM glycoprotein preparations by rabbit anti-N, has structural properties which are similar to those of the MN receptors. Sialic acid, amino groups and carbohydate, susceptible to periodate oxidation, are not involved in the Ss antigen sites. The specificity of theVicia graminea lectin is dependent on free amino and carboxyl groups. Its affinity for the substances is increased by blocking of amino groups.

Zusammenfassung

Untersuchungen mit modifizierten Sialoglykoproteinen aus menschlichen Erythrozyten zeigen, da\ alkali-labile NeuraminsÄure und Aminogruppen Bestandteile der Erythrozyten-Rezeptoren für gewöhnliche Anti-M und -N-Seren von Kaninchen und Menschen darstellen. Das „N“-Antigen, das durch Kaninchen Anti-N in MM-Glykoproteinpreparationen nachweisbar ist, hat Ähnliche strukturelle Eigenschaften wie die MN-Rezeptoren. Am Aufbau der Ss-Antigene sind NeuraminsÄure, durch Perjodat oxidierbares Kohlenhydrat und Aminogruppen nicht beteiligt. Die SpezifitÄt desVicia graminea Lektins hÄngt von freien Amino- und Carboxylgruppen ab. Seine AffinitÄt zu den Glykoproteinen wird durch Blockierung von Aminogruppen gesteigert.

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References

  1. Anstee D. J. & Tanner M. J. A.: The nature of the membrane defect in En(a-) human erythrocytes. XIV Congress of the International Society of Blood Transfusion, Helsinki, July 27–August 1 (1975).

  2. Dahr P.: Zur Serologie der agglutinablen Blutkörperchenmerkmale M und N.Z. Immunforsch. 105, 383 (1945).

    CAS  Google Scholar 

  3. Dahr W. & Uhlenbruck G.: Immunochemical aspects of the MN blood group system. XIV Congress of the International Society of Blood Transfusion, Helsinki, July 27–August 1 (1975).

  4. Dahr W. & Uhlenbruck G.: Immunchemie des MN Systems. VI Congress of the Society of Human Blood Group Science in view of forensic aspects, Innsbruck, October 2–4 (1975).

  5. Dahr W., Uhlenbruck G. & Bird G. W. G.: Cryptic A-like receptor sites in human erythrocyte glycoproteins: proposed nature of Tn-antigen.Vox Sang. 27, 29 (1974).

    Article  CAS  PubMed  Google Scholar 

  6. Dahr W., Uhlenbruck G. & Bird G. W. G.: Further characterization of some heterophile agglutinins reacting with alkali-labile carbohydrate chains of human erythrocyte glycoproteins.Vox Sang. 28, 133 (1975).

    Article  CAS  PubMed  Google Scholar 

  7. Dahr W., Uhlenbruck G. & Bird G. W. G.: Influence of free amino and carboxyl groups on the specificity of plant anti-N.Vox Sang. 28, 389 (1975).

    Article  CAS  PubMed  Google Scholar 

  8. Dahr W., Uhlenbruck G., Gunson H. H. & van der Hart M.: Molecular basis of Tn-polyagglutinability.Vox Sang. 29, 36 (1975).

    Article  CAS  PubMed  Google Scholar 

  9. Dahr W., Uhlenbruck G. & Knott H.: Immunochemical aspects of the MNSs-blood group system.J. Immunogenetics 2, 87 (1975).

    Article  Google Scholar 

  10. Dahr W., Uhlenbruck G., Issitt P. I. & Allen Jr. F. H.: SDS-polyacrylamide gel electrophoretic analysis of the membrane glycoproteins from S-s-Uerythrocytes.J. Immunogenetics 2, 249 (1975).

    Article  CAS  Google Scholar 

  11. Ebert W., Metz J. & Roelcke D.: Modifications of N-acetylneuraminic acid and their influence on the antigen activity of erythrocyte glycoproteins.Eur. J. Biochem. 27, 470 (1973).

    Article  Google Scholar 

  12. Eshhar Z., Gafni M., Givol D. & Sela M.: Solubilization of lymphcyte and thymocyte antigens by a reversible chemical modification.Eur. J. Immunol. 1, 323 (1971).

    Article  CAS  PubMed  Google Scholar 

  13. Fujita S. & Cleve H.: Isolation and partial characterization of two minor glycoproteins from human erythrocyte membranes.Biochim. biophys. Acta 278, 271 (1975).

    Google Scholar 

  14. Gahmberg C., MyllylÄ G. & Nordling S.: Anomalous glycoprotein pattern in human red cell variants En(a-) and Tn. XIV Congress of the International Society of Blood Transfusion, Helsinki, July 27–August 1 (1975).

  15. Glöckner W. M., Newman R. A. & Uhlenbruck G.: Carbohydrate structure and serological behaviour of „antifreeze“ glycoproteins from an antarctic fish.Biochem. biophys. Res. Commun. 66, 701 (1975).

    Article  PubMed  Google Scholar 

  16. Hamaguchi H. & Cleve H.: Solubilization of human erythrocyte membrane glycoproteins and separation of the MN glycoprotein from a glycoprotein with I, S and A activity.Biochim. biophys. Acta 278, 271 (1972).

    Article  CAS  PubMed  Google Scholar 

  17. Kornfeld R. & Kornfeld S.: The structure of a phytohaemagglutinin receptor site from human erythrocytes.J. biol. Chem. 245, 2536 (1970).

    CAS  PubMed  Google Scholar 

  18. Krüpe M. & Uhlenbruck G.: Crypt-antigenic NVg receptor in mucoids from Mu/Mu cells.Vox Sang. 10, 326 (1965).

    Article  Google Scholar 

  19. Lisowska E.: The degradation of M and N blood group glycoproteins and glycopeptides with alkaline borohydride.Eur. J. Biochem. 10, 574 (1969).

    Article  CAS  PubMed  Google Scholar 

  20. Lisowska E. & Morawiecki A.: The role of free amino groups in the blood group activity of M and N mucoids.Eur. J. Biochem. 3, 237 (1967).

    Article  CAS  PubMed  Google Scholar 

  21. Lisowska E. & Duk M.: Modification of amino groups of human erythrocyte glycoprotein and the new concept on the structural basis of M and N blood group specificity.Eur. J. Biochem. 54, 469 (1975).

    Article  CAS  PubMed  Google Scholar 

  22. Lisowska E., Duk M. & Kordowicz M.: Problem of the structural differentiation of M and N blood group antigens. XIV Congress of the International Society of Blood Transfusion, Helsinki, July 27 – August 1 (1975).

  23. Lisowska E., Dzierzkowa-Borodej W., Seyfried H. & Drzeniek Z.: Reactions of erythrocyte glycoproteins and their degradation products with various anti-I sera.Vox Sang. 28, 122 (1975).

    Article  CAS  PubMed  Google Scholar 

  24. Rammler D. H. & Rabinowitz J. C.: A procedure for the microdetermination of formic acid in periodate oxidation mixtures.Anal. Biochem. 4, 116 (1962).

    Article  CAS  PubMed  Google Scholar 

  25. Springer G. F., Tegtmeyer H. & Huprikar S. V.: Anti-N reagents in elucidation of the genetical basis of human blood group MN specificities.Vox Sang. 22, 325 (1972).

    Article  CAS  PubMed  Google Scholar 

  26. Thomas D. B. & Winzler R. J.: Structural studies on human erythrocyte glycoproteins. Alkali-labile oligosaccharides.J. biol. Chem. 244, 5943 (1969).

    CAS  PubMed  Google Scholar 

  27. Uhlenbruck G.: Studien an MN-blut-gruppenaktiven Mucoiden aus Erythrocyten.Zbl. Bakt. 181, 283 (1961).

    CAS  Google Scholar 

  28. Verschuer O.: Zur Frage der Erblichkeit der Blutkörperchenmerkmale M und N.Z. Immunforsch. 105, 392 (1945).

    Google Scholar 

  29. Warren L.: The thiobarbituric acid assay of sialic acids. J. biol. Chem.234, 1971 (1959).

    CAS  PubMed  Google Scholar 

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Author notes

  1. Gerhard Uhlenbruck & Wolfgang Dahr

    Present address: Abteilung für Immunbiologie der Medizinischen UniversitÄtsklinik Köln, Deutschland

Authors and Affiliations

  1. Abteilung für Immunbiologie der Medizinischen UniversitÄtsklinik Köln, Deutschland

    Renate Schmalisch & Eveline Jan\en

Authors
  1. Gerhard Uhlenbruck
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  2. Wolfgang Dahr
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  3. Renate Schmalisch
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  4. Eveline Jan\en
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Additional information

Herrn Prof. Dr. Peter Dahr zum 70. Geburtstag gewidmet.

Part of the work presented in this paper was first reported at the XIV Congress of the International Society of Blood Transfusion, Helsinki, July 27 to August 1, 1975.

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Uhlenbruck, G., Dahr, W., Schmalisch, R. et al. Studies on the receptors of the MNSs blood group system. Blut 32, 163–170 (1976). https://doi.org/10.1007/BF00995909

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