Neurochemical Research

, Volume 20, Issue 9, pp 1041–1048 | Cite as

Bradykinin stimulates phospholipase D in PC12 cells by a mechanism which is independent of increases in intracellular Ca2+

  • Joel Horwitz
  • Brent Passarello
  • Mark Corso
Article

Abstract

These experiments were designed to learn the role of bradykinin induced changes in intracellular Ca2+ in the activation of phospholipase D activity in PC12 cells. Ionomycin at a concentration of 0.1μM caused an increase in intracellular Ca2+ comparable to bradykinin, but had no effect on phospholipase D activity. Carbachol, ATP, and thapsigargin also increased intracellular Ca2+ but had no effect on phospholipase D activity. Increases in intracellular Ca2+ may be a necessary but not a sufficient factor in the activation of phospholipase D. To investigate this issue, the bradykinin induced increase in intracellular Ca2+ was blocked by preincubating the cells in Ca2+-free media plus EGTA or in media containing the intracellular Ca2+ chelator BAPTA/AM. These preincubations completely blocked the bradykinin induced increase in intracellular Ca2+ but only attenuated the bradykinin mediated activation of phospholipase D. Physiological increases in intracellular Ca2+ apparently do not mediate the effect of bradykinin on phospholipase D.

Key words

Phospholipase D PC12 cells bradykinin Ca2+ 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Joel Horwitz
    • 1
  • Brent Passarello
    • 1
  • Mark Corso
    • 1
  1. 1.Department of PharmacologyMedical College of PennsylvaniaPhiladelphia

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