Neurochemical Research

, Volume 20, Issue 10, pp 1239–1248 | Cite as

Biosynthesis and compartmentalization of Po, apolipoprotein A-I, and lipids in the myelinating chick sciatic nerve

  • M. Joanne Lemieux
  • Catherine Mezei
  • W. Carl Breckenridge
Original Articles

Abstract

Myelin deposition in developing chick sciatic nerve is associated with rapid synthesis of lipids, the major myelin protein Po and apo A-I, a major constituent of plasma lipoproteins. In order to understand possible roles of apo A-I in myelin assembly the synthesis and appearance of Po, apo A-I and lipids was studied in an intracellular fraction, an intralamellar fraction thought to be related to, or derived from, myelin and compact myelin from rapidly myelinating sciatic nerve of 1 day chicks. Incorporation with methionine or pulse-chase experiments indicated that initial synthesis of Po occurs in the intracellular fraction followed by movement to the intralamellar fraction and myelin. Incorporation of labelled oleate into phospholipids suggested that initial synthesis occurs in the intracellular and intralamellar fractions with slow movement to myelin. Incorporation of labelled galactose into cerebrosides suggested that initial synthesis occurs partially in myelin with slow loss from this fraction to the intralamellar fraction. However, incorporation of methionine into apo A-I indicated that initial synthesis occurred in the intracellular fraction with some transfer to the intralamellar fraction and secretion of a major portion into the incubation medium. It is concluded that the subcellular distribution of nascent apo A-I is not well coordinated with the distribution of other nascent constitutents of the myelin membrane. The accumulation of nascent Po, phospholipids and cerebrosides in the intralamellar fraction compared to compact myelin suggests that this fraction may play a role as a precursor membrane or as a storage site for assembly of myelin constituents into compact myelin.

Key Words

Apolipoprotein A-I Po avian myelination PNS 

Abbreviations

PAGE

polyacrylamide gel electrophoresis

SDS

sodium dodecyl sulphate

apo

apolipoprotein

PC

phosphatidylcholine

PE

phosphatidylethanolamine

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • M. Joanne Lemieux
    • 1
  • Catherine Mezei
    • 1
  • W. Carl Breckenridge
    • 1
  1. 1.Department of BiochemistryDalhousie UniversityHalifaxScotia

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