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Effect of prenatal treatment with methylazoxymethanol on carbachol-, norepinephrine- and glutamate-stimulated phosphoinositide metabolism in the neonatal, young, and adult offspring

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Abstract

Carbachol-, norepinephrine- and glutamate-stimulated phosphoinositide metabolism was investigated in the neonatal, young and adult cerebral cortex slices of rats prenatally treated with methylazoxymethanol (MAM) on gestational day 15 (GD15) or GD19. In rat offspring treated on GD15 there was a significant reduction in the accumulation of [3H]inositol phosphates induced by carbachol and a significant increase in the accumulation of [3H]inositol phosphates induced by norepinephrine on day 7, whereas no changes were observed at the other ages. No significant changes, on the other hand, were observed for glutamate-stimulated phosphoinositide metabolism in GD15 treated rats and for carbachol-, norepinephrine- and glutamate-stimulated phosphoinositide metabolism in animals treated on GD19 at any of the different ages evaluated. These results indicate that treatment with MAM on GD15, which results in a marked microencephaly, causes a marked alteration of muscarinic and α1-adrenergic receptor-stimulated phosphoinositide metabolism during brain development and that these alterations undergo adaptive changes in the adult brain.

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Authors and Affiliations

  1. Institute of Pharmacology and Pharmacognosy, University of Urbino, Urbino, Via S. Chiara, 27, 61029, Urbino, Italy

    Walter Balduini, Gabriella Lombardelli, Guidubaldo Peruzzi & Flaminio Cattabeni

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  1. Walter Balduini
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  2. Gabriella Lombardelli
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  3. Guidubaldo Peruzzi
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  4. Flaminio Cattabeni
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Balduini, W., Lombardelli, G., Peruzzi, G. et al. Effect of prenatal treatment with methylazoxymethanol on carbachol-, norepinephrine- and glutamate-stimulated phosphoinositide metabolism in the neonatal, young, and adult offspring. Neurochem Res 20, 1211–1216 (1995). https://doi.org/10.1007/BF00995385

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