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Neurochemical Research

, Volume 20, Issue 1, pp 101–105 | Cite as

Effects of repeated injection of cyclosporin A on pentylenetetrazol-induced convulsion and cyclophilin mRNA levels in rat brain

  • Masato Asanuma
  • Norio Ogawa
  • Sakiko Nishibayashi
  • Yoichi Kondo
  • Akitane Mori
Original Articles

Abstract

To investigate the relationship between the immune system and convulsions in an animal model, we examined the effects of repeated administration with the immunosuppressant cyclosporin A on pentylenetetrazol (PTZ)-induced convulsions and the changes in the mRNA expression of its binding protein cyclophilin in the rat brain. The consecutive administration of cyclosporin A (5 mg/kg s.c., 14 days) significantly aggravated the severity of convulsions induced with PTZ 75 mg/kg i.p. Furthermore, it down-regulated the levels of cyclophilin mRNA in several brain regions and inhibited the PTZ-induced increase of hippocampal cyclophilin mRNA. Compared with the group without PTZ pretreatment or the group treated with chronic vehicle administration after the PTZ-preinjection, chronic cyclosporin A administration after the initial injection of PTZ apparently aggravated convulsions after the second PTZ injection. Interestingly, the increase in hippocampal cyclophilin mRNA observed after a single PTZ injection was not found after the second PTZ injection in the group with PTZ pretreatment. Therefore, these findings suggest that cyclosporin A administered peripherally can affect the central nervous system, and that an immune response associated with the first convulsive episode plays a key role in severity during subsequent attacks.

Key Words

Cyclosporin A pentylenetetrazol convulsion seizures cyclophilin rat brain 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Masato Asanuma
    • 1
  • Norio Ogawa
    • 1
  • Sakiko Nishibayashi
    • 1
  • Yoichi Kondo
    • 1
    • 2
  • Akitane Mori
    • 1
  1. 1.Department of Neuroscience, Institute of Molecular and Cellular MedicineOkayama University Medical SchoolOkayamaJapan
  2. 2.Third Department of Internal MedicineOkayama University Medical SchoolOkayamaJapan

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