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Allelopathy of yellow fieldcress (Rorippa sylvestris): Identification and characterization of phytotoxic constituents

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Both the neutral and acidic fractions of the acetone extract of yellow fieldcress (Kireha-inugarashi,Rorippa sylvestris Besser) inhibited lettuce seed germination. Salicylic,p-hydroxybenzoic, vanillic, and syringic acid were identified in the acidic fraction. In the neutral fraction, hirsutin (8-methylsulfinyloctyl isothiocyanate), 4-methoxyindole-3-acetonitrile, and pyrocatechol were identified. Bioassay using a root exudate recirculating system showedR. sylvestris during flowering inhibited the lettuce seedling growth. Hirsutin (13μg/plant/day) and pyrocatechol (9.3μg/plant/day) were the major compounds released into the rhizosphere. Several combinations of pyrocatechol,p-hydroxybenzoic acid, vanillic acid, and hirsutin reduced lettuce seedling growth. These compounds seemed to be allelochemicals.

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Yamane, A., Nishimura, H. & Mizutani, J. Allelopathy of yellow fieldcress (Rorippa sylvestris): Identification and characterization of phytotoxic constituents. J Chem Ecol 18, 683–691 (1992).

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