Skip to main content

Phylogenetic relationships ofSphaerophoraceae (Ascomycetes) inferred from SSU rDNA sequences

Abstract

SSU rDNA was sequenced from the lichenized fungiBunodophoron scrobiculatum andLeifidium tenerum (Sphaerophoraceae), andStereocaulon ramulosum andPilophorus acicularis (Stereocaulaceae) and analysed by maximum parsimony with 44 homologous ascomycete sequences in a cladistic study. A small insertion (c. 60 nt.) was found in the sequence ofLeifidium tenerum. Sphaerophoraceae constitutes a strongly supported monophyletic group which groups together withLecanora dispersa and theStereocaulaceae. Together withPorpidia crustulata, this larger group is a sistergroup to thePeltigerineae. This analysis thus supports theLecanorales as monophyletic, includingSphaerophoraceae and thePeltigerineae, but does not provide strong support for this monophyly. The analysis also suggests that the prototunicate ascus in theSphaerophoraceae is a reversion to the plesiomorphic state. Based on morphological, anatomical and chemical reasons,Sphaerophoraceae is proposed to belong to one of the groups presently included in the paraphyletic suborderCladoniineae within theLecanorales.

This is a preview of subscription content, access via your institution.

References

  • Bremer, K., 1988: The limits of amino acid sequence data in angiosperm phylogenetic reconstruction. — Evolution42: 795–803.

    Google Scholar 

  • , 1994: Branch support and tree stability. — Cladistics10: 295–304.

    Google Scholar 

  • Depriest, P. T., 1992: Molecular genetic analysis of ribosomal DNA polymorphism in theCladonia chlorophaea complex. — Dissertation, Duke University, USA.

    Google Scholar 

  • Eriksson, O., 1981: The families of bitunicateAscomycetes. — Opera Bot.60: 1–220.

    Google Scholar 

  • , 1995: Relationships of the generaNephroma, Peltigera andSolorina (Peltigerales, Ascomycota). — Syst. Ascomycetum14: 33–39.

    Google Scholar 

  • Farris, J. S., 1995: The Parsimony Jackknifer, version 4.22. — Stockholm.

  • , 1996: Parsimony jackknifing outperforms neighbour-joining. — Cladistics12: 99–124.

    Google Scholar 

  • Gargas, A., Depriest, P. T., 1996: A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA. — Mycologia88: 745–748.

    Google Scholar 

  • -Taylor, J. W., 1992a: Molecular systematics of lichenized and non-lichenized fungi based on rDNA sequences. — InKårnefelt, I., (Ed.): IAL 2 Abstract volume: 9–10.

  • , 1992b: Polymerase chain reaction (PCR) primers for amplifying and sequencing nuclear 18S rDNA from lichenized fungi. — Mycologia84: 589–592.

    Google Scholar 

  • , 1995: Phylogeny of discomycetes and early radiations of the apothecialAscomycotina inferred from SSU rDNA sequence data. — Exp. Mycol.19: 7–15.

    Google Scholar 

  • , 1995a: Multiple origin of lichen symbiosis in fungi suggested by SSU rDNA phylogeny. — Science286: 1492–1495.

    Google Scholar 

  • , 1995b: Positions of multiple insertions in SSU rDNA of lichen-forming fungi. — Molec. Biol. Evol.12: 208–218.

    Google Scholar 

  • Grube, M., Gargas, A., Depriest, P. T., 1996: A small insertion in the SSU rDNA of the lichen fungusArthonia lapidicola is a degenerate group-I intron. — Curr. Genet.29: 582–586.

    Google Scholar 

  • Hafellner, J., 1994: Problems inLecanorales systematics. — InHawksworth, D. L., (Ed.): Ascomycete systematics: problems and perspectives in the nineties, pp. 325–320. — New York, London: Plenum Press.

    Google Scholar 

  • , 1994:Lecanorales. — InHawksworth, D. L., (Ed.): Ascomycete systematics: problems and perspectives in the nineties, pp. 379–387. — New York, London: Plenum Press.

    Google Scholar 

  • Henssen, A., Jahns, H. M., 1973 (“1974”):Lichenes. Eine Einführung in die Flechtenkunde. — Stuttgart: Thieme.

    Google Scholar 

  • Kållersjö, M., Farris, J. S., Kluge, G., Bult, C., 1992: Skewness and permutation. — Cladistics8: 275–287.

    Google Scholar 

  • Lee, S. B., Milgroom, M. G., Taylor, J. W., 1988: A rapid, high yield mini-prep method for isolation of total genomic DNA from fungi. — Fungal Genet. Newslett.35: 23–24.

    Google Scholar 

  • , 1990: Isolation of DNA from fungal mycelia and single spores. — InInnis, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J., (Eds): PCR protocols: a guide to methods and applications, pp. 282–287. — London: Academic Press.

    Google Scholar 

  • Mankin, A. S., Skryabin, K. G., Rubtsov, P. M., 1986: Identification of ten additional nucleotides in the primary structure of yeast 18S rRNA. — Gene44: 143.

    Google Scholar 

  • Poelt, J., 1974 (“1973”): Classification. — InAhmadijan, V., Hale, M. E., (Eds): The lichens, pp. 599–632. — London: Academic Press.

    Google Scholar 

  • Rambold, G., Triebel, D., 1992: The inter-lecanoralean associations. — Biblioth. Lichenol.48: 1–201.

    Google Scholar 

  • Rubtsov, P. M., Musakhanov, M. M., Zakharyev, V. M., Krayev, A. S., Skryabin, K. G., Bayev, A. A., 1980: The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of 18S ribosomal RNA gene fromSaccharomyces cerevisiae. — Nucl. Acids Res.8: 5779–5794.

    Google Scholar 

  • Swofford, D. L., 1993: PAUP: phylogenetic analysis using parsimony, version 3.1.1. — Washington: Smithsonian Institution.

    Google Scholar 

  • Tehler, A., 1988: A cladistic outline of theEumycota. — Cladistics4: 227–277.

    Google Scholar 

  • , 1990: A new approach to the phylogeny ofEuascomycetes with a cladistic outline ofArthoniales focussing onRoccellaceae. — Canad. J. Bot.68: 2458–2492.

    Google Scholar 

  • Tibell, L., 1984: A reappraisal of the taxanomy ofCaliciales. — Beih. Nova Hedwigia79: 597–713.

    Google Scholar 

  • Vobis, G., 1980: Bau und Entwicklung der Flechten-Pycnidien und ihrer Conidien. — Biblioth. Lichenol.14: 1–141.

    Google Scholar 

  • Wang-yang, J.-R., Lai, M.-J., 1976: Notes on the lichen genusSphaerophorus Pers. of Taiwan, with description of three new species. — Taiwania21: 83–86.

    Google Scholar 

  • Wedin, M., 1990: Ascocarp and spore ontogeny in two species ofSphaerophorus (Caliciales). — Nordic J. Bot.10: 539–545.

    Google Scholar 

  • , 1993: A phylogenetic analysis of the lichen familySphaerophoraceae (Caliciales); a new generic classification and notes on character evolution. — Pl. Syst. Evol.187: 213–241.

    Google Scholar 

  • , 1995: The lichen familySphaerophoraceae (Caliciales, Ascomycotina) in temperate areas of the Southern Hemisphere. — Symb. Bot. Upsal.31(1: 1–102.

    Google Scholar 

  • White, T. J., Bruns, T., Lee, S., Taylor, J., 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. — InInnis, M. A., Gelfand, D. H., Sninsky, J. J., (Eds): PCR protocols: a guide to methods and applications, pp. 315–322. — London: Academic Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wedin, M., Tehler, A. & Gargas, A. Phylogenetic relationships ofSphaerophoraceae (Ascomycetes) inferred from SSU rDNA sequences. Pl Syst Evol 209, 75–83 (1998). https://doi.org/10.1007/BF00991525

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00991525

Key words

  • Fungi
  • Ascomycetes
  • Caliciales
  • Lecanorales
  • Sphaerophoraceae
  • Stereocaulaceae
  • Lichens
  • molecular evolution
  • phylogeny
  • small subunit
  • ribosomal DNA
  • 18S rDNA