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Molecular Biology Reports

, Volume 20, Issue 3, pp 155–161 | Cite as

A novel human type I hair keratin gene: evidence for two keratin hHa3 isoforms

  • Michael A. Rogers
  • Jürgen Schweizer
  • Thomas Krieg
  • Hermelita Winter
Research Articles

Abstract

We present the nucleotide and amino acid sequence for a novel human type I hair keratin, which could be identified through its high sequence homology and strict carboxyterminal length identity as a human ortholog of the murine hair keratin mHa3. Our hHa3 sequence differs, however, from that of a previously described hHa3 hair keratin (published only as an amino acid sequence; [13]) in 24 amino acid positions, 8 of which occur in the middle of the carboxyterminal domain. PCR of genomic DNA from 25 normal human subjects using a primer pair derived from sequence segments located in the 3′-region of our hHa3 clone that encode conserved amino acid sequences in both keratins, resulted in the amplification of two distinct products of 0.38 kbp and 1.0 kbp. DNA sequence analysis of the cloned PCR products allowed identification of the 0.38 kb sequence as that originating from Yuet al. [13] and the 1.0 kb sequence as that being derived from our data. The difference in fragment length was due to unique intron 6 sequences, indicating that these two keratin species are encoded by genes of their own. Moreover, extensive Southern blot analyses with DNA from 25 unrelated individuals of different races using a 3′-noncoding sequence from our keratin and the intron 6 sequence of the keratin of Yuet al. [13], as hybridization probes showed that both keratin genes are present as single copy sequences occurring ubiquitously and without gross alterations in the human genome. Collectively, these data demonstrate that the human type I hair keratin described in this paper represents an isoform of the previously described hHa3 keratin. We propose that these hHa3 isoforms be named in chronological order of discovery hHa3-I and hHa3-II.

Key words

human hair keratins Sequence homology keratin isoforms 

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References

  1. 1.
    Heid HW, Werner E & Franke WW (1986) Differentiation 32: 101–119PubMedGoogle Scholar
  2. 2.
    Lynch MH, O'Guin WM, Hardy C, Mak L & Sun T-T (1986) J. Cell. Biol. 103: 2593–2606PubMedGoogle Scholar
  3. 3.
    Heid HW, Moll I & Franke WW (1988) Differentiation 37: 137–157PubMedGoogle Scholar
  4. 4.
    Heid HW, Moll I & Franke WW (1988) Differentiation 37: 215–230PubMedGoogle Scholar
  5. 5.
    Powell BC & Rogers GE (1986) In: Bereiter-Hahn JE, Matoltsy AG & Richards KS (Eds) Biology of the Integument (pp 695–721) Springer-Verlag, New YorkGoogle Scholar
  6. 6.
    Powell BC, Crocher L & Rogers GE (1992) Development 114: 417–433PubMedGoogle Scholar
  7. 7.
    Powell BC & Beltrame JS (1994) J. Invest. Dermatol. 102: 171–177PubMedGoogle Scholar
  8. 8.
    Bertolino AP, Checkla DM, Nottermann R, Sklaver I, Schiff TA, Freedberg IM & DiDona GJ (1988) J. Invest. Dermatol. 91: 541–546PubMedGoogle Scholar
  9. 9.
    Bertolino AP, Checkla DM, Heitner S, Freedberg JM & Yu DW (1990) J. Invest. Dermatol. 94: 293–303Google Scholar
  10. 10.
    Kaytes PS, McNab AR, Rea TJ, Groppi V, Kawabe TT, Buhl AE, Bertolino AP, Hatzenbuhler NT & Vogeli G (1991) J. Invest. Dermatol. 97: 835–842PubMedGoogle Scholar
  11. 11.
    Winter H, Siry P, Tobiasch E & Schweizer J (1994) Exp. Cell. Res. 212: 190–200PubMedGoogle Scholar
  12. 12.
    Wilson BW, Edwards KJ, Sleigh MJ, Byrne CR & Ward KA (1988) Gene 73: 21–31PubMedGoogle Scholar
  13. 13.
    Yu J, Yu DW, Checkla DM, Freedberg IM & Bertolino AP (1993) J. Invest. Dermatol. 101: 565–595Google Scholar
  14. 14.
    Rogers MA, Nischt R, Korge B, Krieg T, Fink TM, Lichter P, Winter H & Schweizer J (1995) Exp. Cell. Res. (submitted).Google Scholar
  15. 15.
    Sanger F, Nicklen S & Coulson AR (1977) Proc. Natl. Acad. Sci. USA 75: 5463–5467Google Scholar
  16. 16.
    Waltering S (1994). Murine Haarkeratingene: Mutationsanalysen in Mäusestämmen mit normalem und defektem Haarkleid. Diploma Thesis, Faculty of Biology, University of HeidelbergGoogle Scholar
  17. 17.
    Yu DW, Pang YY, Checkla DM, Freedberg IM, Sun T-T & Bertolino AP (1991) J. Invest. Dermatol. 97: 354–363PubMedGoogle Scholar
  18. 18.
    Tobiasch E, Schweizer J & Winter H (1992) Mol. Biol. Rep. 16: 39–47PubMedGoogle Scholar
  19. 19.
    Compton JG, Ferrara DM, Yu DW, Recca V, Freedberg IM & Bertolino AP (1991) Ann. N.Y. Acad. Sci. 642: 32–43PubMedGoogle Scholar
  20. 20.
    Tyner AL, Eichman MJ & Fuchs E (1985) Proc. Natl. Acad. Sci. USA 82: 4683–4687PubMedGoogle Scholar
  21. 21.
    Takahashi K, Paladini R & Coulombe P (1994) Cloning and functional characterization of multiple type II keratin K6 isoforms in human. J. Invest. Dermatol. 102: 541Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Michael A. Rogers
    • 1
    • 2
  • Jürgen Schweizer
    • 1
    • 2
  • Thomas Krieg
    • 1
    • 2
  • Hermelita Winter
    • 1
    • 2
  1. 1.Research Program 2German Cancer Research CenterHeidelberg (MAR, JS, HW)Germany
  2. 2.Department of DermatologyUniversity of Cologne (TK)Germany

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