Abstract
Nearly terminal sites of Feulgen achromasia coincide with nearly terminal sites of silver banding in the short arms of the subtelocentric pair of chromosomes ofA. cepa. Autoradiographic silver grains after in situ rRNA/DNA hybridization cover a more extended area at this site, thereby giving the molecular basis for the staining results. From these staining results we conclude that the transcriptionally active rRNA genes of the standard nucleolar chromosomes are located in the secondary constriction.
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Battaglia, E., 1957:Allium ascalonicum L.,A. fistulosum L.,A. cepa L.: Analisi cariotipica. — CaryologiaX, 1–28.
Bleecken, S., 1961: Untersuchungen des autoradiographischen Auflösungsvermögens mit Strahlungsquellen verschiedener Betaenergien. — Atompraxis7, 321–324.
Bloom, S. E., Goodpasture, C., 1976: An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. — Hum. Genet.34, 199–206.
Bougourd, S. M., Parker, J. S., 1976: Nucleolar-organizer polymorphism in natural populations ofAllium schoenoprasum. — Chromosoma (Berl.)56, 301–307.
Burger, E.-Ch., Knälmann, M., 1980: Koinzidenz von Feulgen-Achromasie, in situ Hybridisierung und Silberbandenfärbung in vier Nukleolusorganisatoren vonVicia sativa. — Europ. J. of Cell Biol.21, 313–318.
Dyer, A. F., 1963: Allocyclic segments of chromosomes and the structural heterozygosity that they reveal. — Chromosoma (Berl.)13, 545–576.
Hizume, M., Sato, S., Tanaka, A., 1980: A highly reproducible method of nucleolus organizing regions staining in plants. — Stain Technol.55, 87–90.
Maggini, F., Barsanti, P., Marazia, T., 1978: Individual variation of the nucleolus organizer regions inAllium cepa andA. sativum. — Chromosoma (Berl.)66, 173–183.
Noda, S., 1953: A new typed nucleolar chromosome in a supernumerary fragment chromosome inAllium cepa L. — Mem. Fac. Science, Kyushu Univ. Ser. E1, 139–146.
Sato, S., Hizume, M., Kawamura, S., 1980: Relationship between secondary constriction and nucleolus organizing regions inAllium sativum chromosomes. — Protoplasma105, 77–85.
Sekerka, V., 1977: Karyotype of a tissue culture ofAllium cepa L. in vitro. — Acta F. R. N. Univ. Comen. — Physiologia PlantarumXIII, 43–46.
Stack, S. M., 1974: Differential Giemsa staining of kinetochores and nucleolus organizer heterochromatin in mitotic chromosomes of higher plants. — Chromosoma (Berl.)47, 361–378.
, 1974: Different kinds of heterochromatin in higher plant chromosomes. — J. Cell Sci.14, 499–504.
Tjio, J. H., Levan, A., 1956: An interesting formation of one satellited chromosome in an individual ofAllium cepa. — Anal. Aula Dei4, 185–190.
Vosa, C. G., 1976: Heterochromatin patterns inAllium. 1. The relationship between the species of thecepa group and its allies. — Heredity36, 383–392.
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Herrn Prof. Dr.G. M. Hoffmann zum 60. Geburtstag gewidmet.
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Burger, EC., Knälmann, M. Localization of ribosomal gene sites in the standard nucleolar chromosomes ofAllium cepa . Pl Syst Evol 142, 117–122 (1983). https://doi.org/10.1007/BF00989607
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DOI: https://doi.org/10.1007/BF00989607