Abstract
A rapid, sensitive, stable, and quantitative high-pressure liquid chromatographic technique was developed for the analysis of the norsesquiterpenes, gyrinidal, isogyrinidal, gyrinidone, and gyrinidione. Methods were developed for both normal-phase (μPorasil) and reverse-phase (μBondapak) columns. The applicability of this technique was demonstrated when the norsesquiterpenes of the defensive secretions of the water beetleGyrinus frosti Fall were isolated and identified as gyrinidal and isogyrinidal. This method was then used for the quantification of the norsesquiterpene titer ofG. frosti andDineutus assimilis (Kirby).
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Newhart, A.T., Mumma, R.O. High-pressure liquid chromatographic techniques for the separation and quantification of norsesquiterpenes from gyrinids. J Chem Ecol 4, 503–510 (1978). https://doi.org/10.1007/BF00988914
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DOI: https://doi.org/10.1007/BF00988914