Abstract
In this report data are summarized on changes in the quantity of proteolipid protein (PLP), its amino acid composition, and the lipid moiety of these lipid-protein complexes in rat brain during postnatal development. In all three parts of the central nervous system (CNS) studied (cerebral hemispheres, medulla oblongata and spinal cord) the main pattern of PLP accumulation is on the whole similar. PLP content is very low in the newborn, and it increased 12 to 20-fold during development. The highest rate of PLP accumulation is observed in the periodfrom 10 to 30 days after birth. Against the background of general similarity the concentration of some amino acids such as lysine, proline, tyrosine in PLP somewhat increased during development, while that of aspartic acid, glutamic acid, glycine, and leucine decreased. Soluble proteolipid complexes, purified to various degree from lipids were isolated from brain of rats of different ages. As compared with the original lipid extracts from which they were obtained, the crude and especially purified proteolipids in all the animals studied were enriched in acidic phospholipids (PhL). This prevalence of acidic PhL increased with age. During the development in phospholipid moiety of proteolipids (PL) the content of phosphatidyl serine, sphingomyelin and mainly diphosphatidyl glycerol increases and that of phosphatidyl inositol and especially phosphatidyl choline decreases. The concentration of acidic PhL more tightly bound with PLP appreciably increases with age. Most of these changes occur mainly during the second decade after birth.
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Folch, J., andLees, M. 1951. Proteolipids, a new type of tissue lipoproteins. Their isolation from brain. J. Biol. Chem. 191:807–817.
Folch-Pi, J., andStoffyn, P. J. 1972. Proteolipids from membrane systems. Ann. N. Y. Acad. Sci. 195:86–107.
Lees, M. B., Sakura, D. J., Sapirstein, V. S., andCuratolo W. 1979. Structure and function of proteolipids in myelin and non-myelin membranes. Biochim. Biophys. Acta 559:209–230.
Mehl, E., andHalaris, A. 1970. Stoichiometric relation of protein components in cerebral myelin from different species. J. Neurochem. 17:659–668.
Manukian, K. H., Kirakosian, L. G., Levonian, K. L., andStepanian, A. A. 1976. Proteolipids of rat brain myelin. Pages 116–128,in Buniatian, H. Ch. (ed.), Vopr. Biochim. Mozga. Vol. 11, Yerevan.
Lapetina, E. G., Soto, E. F., andDe Robertis, E. 1968. Lipids and proteolipids in isolated subcellular membranes of rat brain cortex. J. Neurochem. 15:437–445.
Manukian, K. H., Levonian, K. L., Stepanian, A. A., Kirakosian, L. G., andKazarian, T. I. 1977. The subcellular distribution of proteolipids in rat brain. Pages 68–80,in Buniatian, H. Ch. (ed.), Vopr. Biochim. Mozga. Vol. 12, Yerevan.
Folch, J. 1955. Composition of the brain in the relation to maturation. Pages 121–136,in Welch, H. (ed.), Biochemistry of the Developing Nervous System, Academic Press, New York.
Greaney, J. F. 1961. The chemical maturation of rat brain. Fed. Proc. 20:343.
Greenfield, S., Norton, W. T., andMorell, P. 1971. Quaking mouse: isolation and characterization of myelin protein. J. Neurochem. 18:2119–2128.
Agrawal, H. C., Burton, R. M., Fishman, M. A., Mitchell, R. F., andPrensky, A. L. 1972. Partial characterization of a new myelin protein component. J. Neurochem. 19:2083–2089.
Waehneldt, T. V., andNeuhoff, V. 1974. Membrane proteins of rat brain: compositional changes during postnatal development. J. Neurochem. 23:71–77.
Gomez, C. J., Pasquini, J. M., Soto, E. F., andDe Robertis, E. 1970. Binding of d-[dimethyl-14C]tubocurarine, acetylcholinesterase and proteolipids in subcellular membranes of cerebral cortex of the developing rat. J. Neurochem. 17:1485–1492.
Prensky, A. L., andMozer, H. W. 1967. Changes in the amino acid composition of proteolipids of white matter during maturation of the human nervous system. J. Neurochem. 14:117–121.
Sabri, M. I., Bone, A. H., andDavison, A. N. 1974. Turnover of myelin and other structural proteins in the developing rat brain. Biochem. J. 142:499–507.
Agrawal, H. C., Fujimoto, K., andBurton, R. M. 1976. Accumulation and turnover of the classical Folch-Lees proteolipid protein in developing and adult rat brain. Biochem. J. 154:265–269.
Benjamins, J. A., Iwata, R., andHazlett, J. 1978. Kinetics of entry of proteins into the myelin membrane. J. Neurochem. 31:1077–1085.
Greenfield, S., Williams, N. I., White, M., Brostoff, S. W., andHogan, E. L. 1979. Proteolipid protein: synthesis and assembly into quaking mouse myelin. J. Neurochem. 32:1647–1651.
Townsend, L. E., Agrawal, D., Benjamins, J. A., andAgrawal, H. C. 1982. In vitro acylation of rat brain myelin proteolipid protein. J. Biol. Chem. 257:9745–9750.
Townsend, L. E., andBenjamins, J. A. 1983. Effects of monensin on posttranslational processing of myelin proteins. 1983. J. Neurochem. 40:1333–1339.
Kreps, E. M. 1981. Lipids of cell membranes. Nauka. Leningrad.
Manukian, K. H. 1967. The composition of the phospholipid moiety of the brain grey and white matter proteolipids. Pages 177–190,in Buniatian, H. Ch. (ed.), Vopr. Biochim. Mozga, Vol 3, Yerevan.
Folch-Pi, J. 1973. Proteolipids. Pages 45–66,in Schneider, D. J. (ed.), Proteins of the Nervous System, Raven Press, New York.
Manukian, K. H. 1979. Proteolipids of the nervous system. Pages 86–106,in Buniatian, H. Ch. (ed.), Vopr. Biochim. Mozga, Vol. 13, Yerevan.
Manukian, K. H. 1982. Lipid moiety of proteolipids from various parts of nervous system and brain subcellular particles. Neurochimia 1:51–65.
Manukian, K. H. 1978. Proteolipids of rat brain during ontogenesis. Page 65, The All-Union Conference “Evolutionary Biochemistry and Origin of Life”, Yerevan.
Kirakosian, L. G. 1981. Quantitative changes of proteolipid protein in various parts of nervous system and some other organs or rat during ontogenesis. Biol. J. Armenia 34:538–539.
Kirakosian, L. G., andManukian, K. H. 1982. Amino acid composition of proteolipids from the brain and heart of rats of different ages. J Exp. Clin. Med., Acad. Sci. Armenian SSR 22:209–213.
Folch, J., Lees, M., andSloane-Stanley, G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497–509.
Lowry, O. J., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin reagent. J. Biol. Chem. 193:265–275.
Hess, H. H., andLewin, E. 1965. Microassay of biochemical structural components in nervous tissues. II Methods for cerebrosides, proteolipid proteins and residue proteins. J. Neurochem. 12:205–211.
Lees, M. B., andPaxman, S. 1972. Modification of the Lowry procedure for the analysis of proteolipid protein. Anal. Biochem. 47:184–192.
Folch-Pi, J., Webster, G. R., andLees, M. 1959. The preparation of proteolipids. Fed. Proc. 18:228.
Manukian, K. H., Kirakosian, L. G., andLevonian, K. L. 1972. Content and lipid composition of proteolipids of the white matter of different parts of brain and spinal cord and sciatic nerve of dogs. Page 140–149,in Buniatian, H. Ch. (ed.) Vopr. Biochim. Mozga, Vol. 7, Yerevan.
Blumenkantz, N. 1980. Three automated quantitative assays for serum proteins. Clin. Biochem. 13:62–66.
Smirnov, A. A., Chirkovskaya, E. V., andManukian, K. H. 1961. A study of brain phosphatides by chromatography on silicic acid impregnated paper. Biochimia 26:1027–1033.
Vanderkooi, G., andCapaldi, R. A. 1972. A comparative study of the amino acid compositions of membrane proteins and other proteins. Ann. N. Y. Acad. Sci. 195:135–138.
Kreps, E. M., Patrikeeva, M. V., Smirnov, A. A., Chirkovskaya, E. V., andVaver, V. A. 1969. The sulfatide nature of so called x-lipid of brain. J. Evol. Biochem. Physiol. 5:360–364.
Manukian, K. H., Stepanian, A. A., Harutjunian, D. L., Kirakosian, L. G. 1981. Amino acid composition of proteolipids from different subcellular particles of rat brain. Biol. J. Armenia 34:878–880.
Soto, E. F., Bizzozero, O. A., andPasquini, J. M. 1981. Incorporation of3H palmitic acid into myelin and microsomal proteolipids. Proc. Int. Soc. Neurochem. 8:26.
Bizzozero, O. A., Soto, E. F., andPasquini, J. M. 1983. Myelin proteolipid protein is not esterified at the site of synthesis. Neurochem. Int. 5:729–736.
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Special Issue dedicated to Dr. Eugene Kreps.
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Manukian, K.H., Kirakosian, L.G. Proteolipids in developing rat brain. Neurochem Res 10, 1533–1545 (1985). https://doi.org/10.1007/BF00988865
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DOI: https://doi.org/10.1007/BF00988865