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Molecular Biology Reports

, Volume 22, Issue 2–3, pp 171–175 | Cite as

RNase P as hyperprocessing enzyme: A model for formation of a biologically functional tRNA fragment

  • Yo Kikuchi
Special Issue: RNase MRP/RNase P Systems RNase P

Abstract

Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs inDrosophila cells. In the transposoncopia-related retrovirus-like particles ofDrosophila, a 39-nucleotide-long fragment from the 5′-region ofDrosophila initiator methionine tRNA is used as the primer forcopia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessingin vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure

Key words

Drosophila hyperprocessing primer retrotransposoncopia reverse transcription RNA processing 

Abbreviations

HIV-1

type 1 human immunodeficiency virus

kb

kilobases

LTR

long terminal repeat

PBS

primer binding site

RVLPs

retrovirus-like particles

tRNAiMet

initiator methionine tRNA

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Yo Kikuchi
    • 1
  1. 1.Division of Bioscience and Biotechnology, Department of Ecological EngineeringToyohashi University of TechnologyToyohashiJapan

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