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Structure-function analysis in nuclear RNase P RNA

  • Special Issue: RNase MRP/RNase P Systems
  • RNase P
  • Published:
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Abstract

Eukaryotic ribonuclease P (RNase P) enzymes require both RNA and protein subunits for activityin vivo andin vitro. We have undertaken an analysis of the complex RNA subunit of the nuclear holoenzyme in an effort to understand its structure and its similarities to and differences from the bacterial ribozymes. Phylogenetic analysis, structure-sensitive RNA footprinting, and directed mutagenesis reveal conserved secondary and tertiary structures with both strong similarities to the bacterial consensus and distinctive features. The effects of mutations in the most highly conserved positions are being used to dissect the functions of individual subdomains.

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Abbreviations

RPRI :

ribonucleaseP ribonucleoprotein 1 gene fromSaccharomyces cerevisiae

Pu:

purine ribonucleoside

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Authors and Affiliations

  1. Department of Biological Chemistry, The University of Michigan, 48109-0606, Ann Arbor, MI, USA

    David R. Engelke, Eileen Pagán-Ramos & Anthony J. Tranguch

Authors
  1. David R. Engelke
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  2. Eileen Pagán-Ramos
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  3. Anthony J. Tranguch
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Engelke, D.R., Pagán-Ramos, E. & Tranguch, A.J. Structure-function analysis in nuclear RNase P RNA. Mol Biol Rep 22, 157–160 (1995). https://doi.org/10.1007/BF00988722

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