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Allotetraploid origin ofAllium altyncolicum (Alliaceae, Allium sect.Schoenoprasum) as investigated by karyological and molecular markers

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Abstract

The tetraploidAllium altyncolicum (2n = 4x = 32) is considered to be of hybrid origin, because most of its morphological characters are intermediate between those of its putative parents,A. schoenoprasum andA. ledebourianum. In the present work an attempt has been made to ascertain its parentage by several methods: Giemsa C-banding, genomic in situ hybridization (GISH), PCR-RFLP of cpDNA, restriction enzyme mapping of the rDNA, and RAPDs. C-banding and GISH indicates clearly thatA. altyncolicum is a segmental allopolyploid.Allium schoenoprasum andA. ledebourianum are the most likely the parental species and the larger part of the genome ofA. altyncolicum (26 chromosomes) is derived fromA. schoenoprasum. The low genetic divergence between these three species was confirmed by the lack of sequence variation in the ITS sequences of nuclear rRNA genes and of the plastid rbcL-atpB intergenic spacer. Both parental species andA. altyncolicum could be distinguished by RFLP of the rDNA repeats. The geographic origin of the putative parental species was investigated using RAPDs.

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Authors and Affiliations

  1. Institute for Plant Genetics and Crop Plant Research, D-06466, Gatersleben, Germany

    Nikolai Friesen, Nikolai Borisjuk, Ted H. M. Mes, Manfred Klaas & Peter Hanelt

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  1. Nikolai Friesen
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  2. Nikolai Borisjuk
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  3. Ted H. M. Mes
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  4. Manfred Klaas
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  5. Peter Hanelt
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Dedicated to emer. Univ.-Prof. DrFriedrich Ehrendorfer on the occasion of his 70th birthday

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Friesen, N., Borisjuk, N., Mes, T.H.M. et al. Allotetraploid origin ofAllium altyncolicum (Alliaceae, Allium sect.Schoenoprasum) as investigated by karyological and molecular markers. Pl Syst Evol 206, 317–335 (1997). https://doi.org/10.1007/BF00987955

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