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Technique for using microencapsulated terpenes in lepidopteran artificial diets

Journal of Chemical Ecology volume 18, pages 543–560 (1992)Cite this article

Abstract

We have developed and tested a technique to microencapsulate terpene compounds by forming gelatin-walled microcapsules around the terpene essential oils. Eight terpenes that are common constituents of Douglas fir (Pseudotsuga menziesii) oleoresin were encapsulated, including five monoterpenes (α-pinene,β-pinene, camphene, myrcene, and limonene) and three oxygenated monoterpenes (bornyl acetate, linalool, andβ-citronellol). The encapsulated terpenes were mixed into artificial diets to determine the effects they had on western spruce budworm (Choristoneura occidentalis) survival and reproduction, using a three-generation bioassay. We describe the technique and present data from preliminary bioassays with seven of the terpenes to demonstrate thatC. occidentalis larvae would consume diets fortified with microencapsulated terpenes. The technique is useful because it helps to reduce the rate at which terpenes volatilize from the diets and it “packages” the terpenes in a manner more closely resembling the way they are present in the resin canals of plant foliage. Many scientists investigating the biological importance of terpenes in mediating plant-herbivore interactions may find that microencapsulated terpenes are useful in both laboratory diet studies and field and greenhouse experiments with plant material.

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Affiliations

  1. Forestry Sciences Laboratory, Rocky Mountain Forest and Range Experiment Station, 86001, Flagstaff, Arizona

    Karen M. Clancy

  2. Department of Chemistry, Northern Arizona University, 86011, Flagstaff, Arizona

    Richard D. Foust

  3. Bilby Research Center, Northern Arizona University, 86011, Flagstaff, Arizona

    Richard D. Foust & Thomas G. Huntsberger

  4. Micros-cent, Inc., 5010 Highway 58, 37416, Chattanooga, Tennessee

    John G. Whitaker & Douglas M. Whitaker

Authors
  1. Karen M. Clancy
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  2. Richard D. Foust
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  3. Thomas G. Huntsberger
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  4. John G. Whitaker
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  5. Douglas M. Whitaker
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Clancy, K.M., Foust, R.D., Huntsberger, T.G. et al. Technique for using microencapsulated terpenes in lepidopteran artificial diets. J Chem Ecol 18, 543–560 (1992). https://doi.org/10.1007/BF00987818

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  • DOI: https://doi.org/10.1007/BF00987818

Key words

  • Artificial diets
  • Choristoneura occidentalism
  • Lepidoptera
  • Tortricidae
  • Douglas fir
  • microencapsulated terpenes
  • monoterpenes
  • multiple generation bioassay
  • nutritional ecology
  • oxygenated monoterpenes
  • Pseudotsuga menziesii
  • western spruce budworm