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Antifouling agents against the benthic marine diatom,Navicula salinicola Homarine from the gorgoniansLeptogorgia virgulata andL. setacea and analogs

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Abstract

At concentrations found in the gorgonian corals,Leptogorgia virgulata (L.) andL. setacea (L.), homarine (N-methyl-2-carboxypyridine) and water-soluble extracts from the gorgonians that contained homarine inhibited the growth of the potential fouling diatom.Navicula salinicola Hust., by 50–60%. Homarine comprised 0.3 and 0.25% of the fresh weight ofL. virgulata andL. setacea, respectively, and the water-soluble extracts comprised 4.0 and 3.0% of the fresh weight of the gorgonians, respectively. Three compounds structurally related to homarine including, in order of most to least active, nicotinic acid, picolinic acid, and pyridine, also reduced growth ofN. salinicola. The activity of these compounds in the diatom assay suggests that the carboxyl group at the 2 position of the pyridine ring is important for activity and thatN-methylation is not important. We conclude that chemical defense against fouling is operative inLeptogorgia species. Evidence from the literature for combined chemical and mechanical defenses byLeptogorgia and other organisms against fouling is presented.

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References

  • Al-Ogily, S.M., andKnight-Jones, E.W. 1977. Anti-fouling role of antibiotics produced by marine algae and bryozoans.Nature 265:728–729.

    Google Scholar 

  • Baker, J.T., andMurphy, V. 1976. Handbook of Marine Science: Compounds from Marine Organisms. Vol. 1. CRC Press. Cleveland, Ohio, 226 pp.

    Google Scholar 

  • Bakus, G.J. 1981. Chemical defense mechanisms on the Great Barrier Reef, Australia.Science 211:497–499.

    Google Scholar 

  • Bayer, F.M. 1961. The Shallow WaterOctocorallia of the West Indian Region. Martinus Nijhoff, The Hauge, Netherlands, 373 pp.

    Google Scholar 

  • Burkholder, P.R. 1973. The ecology of marine antibiotics and coral reefs, pp. 117–182,in O.A. Jones and R. Endean (eds.). Biology and Geology of Coral Reefs, Vol. II: Biology I. Academic Press, New York.

    Google Scholar 

  • Burkholder, P.R., andBurkholder, L.M. 1958. Antimicrobial activity of horny corals.Science 127:1174–1175.

    Google Scholar 

  • Chan, A.T., Andersen, R.J., LeBlanc, M.J., andHarrison, P.J. 1980. Algal plating as a tool for investigating allelopathy among marine microalgae.Mar. Biol. 59:7–13.

    Google Scholar 

  • Ciereszko, L.S., 1962. Chemistry of coelenterates. III. Occurrence of antimicrobial terpenoid compounds in the zooxanthellae of alcyonarians.Trans. N. Y. Acad. Sci. 24:502–503.

    Google Scholar 

  • Ciereszko, L. S., andKarns, T.K.B. 1973. Comparative biochemistry of coral reef coelenterates, pp. 188–203.in O.A. Jones and R. Endean (eds.). Biology and Geology of Coral Reefs. Vol. II: Biology 1. Academic Press, New York.

    Google Scholar 

  • Ciereszko, L.S., Sifford, D.H., andWeinheimer, A.J. 1960. Chemistry of coelenterates. I. Occurrence of terpenoid compounds in gorgonians.Ann. N. Y. Acad. Sci. 90:917–919.

    Google Scholar 

  • Ciereszko, L.S., Mizelle, J.W., andSchmidt, R.W. 1973. Occurrence of taurobetaine in coelenterates and of polysaccharide sulfate in the gorgonianPseudolerogorgia americana, pp. 177–180,in L.R. Worthen (ed.). Food-Drugs from the Sea Proceedings 1972. Marine Technology Society. Washington. D.C.

    Google Scholar 

  • Conover, J.T., andSieburth, J.M. 1964. Effect ofSargassum distribution on its epibiota and antibacterial activity.Bot. Mar. 6:147–155.

    Google Scholar 

  • Corpe, W.A., Satsuuchi, L., Armbruster, B. 1976. Secretion of polymers and attachment of marine bacteria to surfaces, pp. 433–442,in J.M. Sharpley and A.M. Kaplan (eds.). Proceedings 3rd International Biodegradation Symposium. Applied Science Publications, London.

    Google Scholar 

  • Dagley, S., andNicholson, D.E. 1970. An Introduction to Metabolic Pathways. John Wiley & Sons, New York, 343 pp.

    Google Scholar 

  • Darley, W.M., Anlman, C.T., andWimpee, B.B. 1979. Utilization of dissolved organic carbon by natural populations of epibenthic salt marsh diatoms.J. Phycol. 15:1–5.

    Google Scholar 

  • Fenical, W., Okuda, R.K., Bandurraga, M.M., Culver, P., andJacobs, R.S. 1981. Lophotoxin: a novelneuromuscular toxin from Pacific sea whips of the genusLophogorgia.Science 212:1512–1514.

    Google Scholar 

  • Filion-Myklebust, C., andMorton, T.A. 1981. Epidermis shedding in the brown seaweedAscophyllum nodosum (L.) Le Jolis, and its ecological significance.Mar. Biol. Lett. 2:45–51.

    Google Scholar 

  • Fuller, J.L. 1946. Season of attachment and growth of sedentary marine organisms at Lamoine, Maine.Ecology87:150–158.

    Google Scholar 

  • Gasteiger, E.L., Gergen, J., andHaake, P. 1955. A study of the distribution of homarine (N-methyl picolinic acid).Biol. Bull. 109:345–346.

    Google Scholar 

  • Geiselman, J.A., andMcConnell, O.J. 1981. Polyphenols in the brown algaeFucus vesiculosus andAscophyllum nodosum: Chemical defenses against the marine herbivorous snail,Littorina littorea.J. Chem. Ecol. 7:1115–1134.

    Google Scholar 

  • Graham, H.W., andGray, H. 1945. Season of attachment and growth of sedentary marine organisms at Oakland, California.Ecology 26:375–386.

    Google Scholar 

  • Grigg, R.W. 1977. Population dynamics of two gorgonian corals.Ecology 58:278–290.

    Google Scholar 

  • Guillard, R.R.L., andRyther, J.H. 1962. Studies on marine planktonic diatoms I.Cyclotella nana Hustedt andDetonula confervacea (Cleve.) Gran.Can. J. Microbiol. 8:229–239.

    Google Scholar 

  • Halstead, B.W. 1965. Poisonous and Venomous Marine Animals of the World, Vol. 1. U.S. Government Printing Office, Washington, D.C., 994 pp.

    Google Scholar 

  • Harrison, P.G., andChan, A.T. 1980. Inhibition of the growth of micro-algae and bacteria by extracts of eelgrass (Zostera marina) leaves.Mar. Biol. 61:21–26.

    Google Scholar 

  • Kato, T.,Kumanireng, A.S.,Ichinose, I.,Kitahara, Y.,Kakinuma, Y., andKato, Y. 1975a. Structure and synthesis of active component from a marine alga,Sargassum tortile, which induces the settling of swimming larvae ofCoryne uchida. Chem. Lett. 335–338.

  • Kato, T., Kumanireng, A.S., Ichinose, I., Kitahara, Y., Kakinuma, Y., Nishihara, M., andKato, M. 1975b. Active components ofSargassum tortile affecting the settlement of swimming larvae ofCoryne uchidai.Experientia 31:433–434.

    Google Scholar 

  • Kinzie, R.A., III. 1973. Zonation of West Indian gorgonians.Bull. Mar. Sci. 22:93–155.

    Google Scholar 

  • Lehninger, A.L. 1970. Biochemistry. Worth Publishers, New York, 833 pp.

    Google Scholar 

  • Marszalek, D.J., Gerehakov, J.M., andUdey, L.R. 1979. Influence of substrate composition or marine microfouling.App. Environ. Microbiol. 38:987–995.

    Google Scholar 

  • McLachlan, J., andCraigie, J.S. 1964. Algal inhibition by yellow ultraviolet-absorbing substances fromFucus vesiculosus.Can. J. Bot. 42:287–292.

    Google Scholar 

  • McLachlan, J., andCraigie, J.S. 1966. Antialgal activity of some simple phenols.J. Phycol. 2:133–135.

    Google Scholar 

  • Ogden, J.C., andLobel, P.S. 1978. The role of herbivorous fishes and urchins in coral reef communities.Environ. Biol. Fish. 3:49–63.

    Google Scholar 

  • O'Neil, T. B., andWilcox, G. L. 1971. The formation of a “primary film” on materials submerged in the sea at Port Hueneme, California,Pac. Sci. 25:1–12.

    Google Scholar 

  • Osman, R.W. 1977. The establishment and development of a marine epifaunal community.Ecol. Monogr. 47:37–63.

    Google Scholar 

  • Patton, W.K. 1972. Studies on the animal symbionts of the gorgonian coral,Leptogorgia virgulata (Lamarck).Bull. Mar. Sci. 22:419–413.

    Google Scholar 

  • Perkins, D.L., andCiereszko, L.S. 1973. The environmental toxicity of crassin acetate usingTetrahymena pyriformis as a model.Hydrobiologica 42:77–84.

    Google Scholar 

  • Preston, E.M., andPreston, J.L. 1975. Ecological structure in a West Indian gorgonian fauna.Bull. Mar. Sci. 25:248–258.

    Google Scholar 

  • Schmitz, F.J., andLorance, E.D. 1971. Chemistry of coelenterates. XXI. Lactones from the gorgonianPterogorgia guadalupensis.J. Org. Chem. 36:719–721.

    Google Scholar 

  • Sieburth, J.M. 1968. The influence of algal antibiosis on the ecology of marine organisms, pp. 63–94,in M.R. Droop and E.J.F. Woods (eds.). Advances in Microbiology of the Sea, Vol. 1. Academic Press, New York.

    Google Scholar 

  • Sieburth, J.M. 1975. Microbial Seascapes. University Park Press, Baltimore.

    Google Scholar 

  • Sieburth, J.M., andConover, J.T. 1965.Sargassum tannin, an antibiotic which retards fouling.Nature 208:52–53.

    Google Scholar 

  • Sokal, R.R., andRohlf, F.J. 1969. Biometry. W.H. Freeman and Co. San Francisco, 776 pp.

    Google Scholar 

  • Stoecker, D. 1978. Resistance of a tunicate to fouling.Biol. Bull. 155:615–626.

    Google Scholar 

  • Sullivan, M.J. 1978. Diatom community structure: Taxonomic and statistical analyses of a Mississippi salt march.J. Phycol. 14:468–475.

    Google Scholar 

  • Tursch, B. 1976. Some recent developments in the chemistry of alcyonaceans.Pure Appl. Chem. 48:1–6.

    Google Scholar 

  • Tursch, B., Braekman, J.C., Daloze, D., andKaisin, M. 1978. Terpenoids from coelenterates. pp. 247–296,in P.J. Scheuer (ed.). Marine Natural Products, Vol. II. Academic Press, New York.

    Google Scholar 

  • Vadas, R.L. 1977. Preferential feeding: An optimization strategy in sea urchins.Ecol. Monogr. 47:337–371.

    Google Scholar 

  • Weinheimer, A.J., andMatson, J.A. 1975. Crassin acetate, the principal antineoplastic agent in four gorgonians of thePseudoplexaura genus.Lloydia 38:378–382.

    Google Scholar 

  • Weinheimer, A.J.,Washecheck, P.H., van derHelm, D.,Bilayet-Hossain, M. 1968. The sesquiterpene hydrocarbons of the gorgonian,Pseudoterogorgia americana, the noniso-prenoid-gorgonene,Chem. Commun. 1070–1071.

  • Weinheimer, A.J., Metzner, E.K., andMole, M.L., Jr. 1973. A new marine betaine, noorzooanemonin, in the gorgonianPseudopterogorgia americana.Tetrahedron 29:3135–3136.

    Google Scholar 

  • Weinheimer, A.J.,Matson, J.A., van derHelm, D., andPoling, M. 1977. Marine anticancer agents: Asperdiol, a cembranoid from the gorgonians,Eunicea asperula andE. tourneforti. Tetrahedron Lett. 1295–1298.

  • Welsh, J.H., andProck, P.B. 1958. Quaternary ammonium bases in the coelenterates.Biol. Bull. 115:551–561.

    Google Scholar 

  • Williams, R.B. 1962. The ecology of diatom populations in a Georgia salt marsh. PhD dissertation, Harvard University, Cambridge, Massachusetts.

    Google Scholar 

  • Williams, R.B. 1964. Division rates of salt marsh diatoms in relation to salinity and cell size.Ecology 45:877–880.

    Google Scholar 

  • Wood, E.J.F. 1950. Investigations on underwater fouling.Aust. J. Mar. Freshwater Res. 1:85–91.

    Google Scholar 

  • Yentsch, C., andMenzel, D.W. 1963. A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence.Deep-Sea Res. 10:221–231.

    Google Scholar 

  • Young, L.Y. andMitchell, R. 1973. The role of chemotactic responses in primary film formation, pp. 617–624,in R.F. Acker, B.F. Brown. J.R. DePalma, and W. P. Iverson (eds.). Proceedings 3rd International Congress on Marine Corrosion and Fouling. Northwestern University Press, Evanston, Illinois.

    Google Scholar 

  • Zapata, O., andMcmillan, C. 1979. Phenolic acids in seagrasses.Aquat. Bot. 7:307–317.

    Google Scholar 

  • Zar, J.H. 1974. Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, New Jersey, 620 pp.

    Google Scholar 

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  1. O. J. McConnell

    Present address: Shell Development Company, P.O. Box 4248, 95352, Modesto, California

Authors and Affiliations

  1. Skidaway Institute of Oceanography, P.O. Box 13687, 31416, Savannah, Georgia

    N. M. Targett, S. S. Bishop, O. J. McConnell & J. A. Yoder

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Targett, N.M., Bishop, S.S., McConnell, O.J. et al. Antifouling agents against the benthic marine diatom,Navicula salinicola Homarine from the gorgoniansLeptogorgia virgulata andL. setacea and analogs. J Chem Ecol 9, 817–829 (1983). https://doi.org/10.1007/BF00987807

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