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Techniques for cloning cDNAs encoding interactive transcriptional regulatory proteins

  • Special Issue: Protein-Protein Interactions
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Summary

Several approaches aimed at detecting and cloning interactive transcriptional regulatory proteins have been presented. All of the techniques can effectively identify specific interactions between two transcription proteins. However, interaction cloning and the two hybrid system have the added advantage of yielding a cDNA expression clone directly. The other methods, EMSA-mediated cloning, co-immunoprecipitation, oligonucleotide/PCR-facilitated cloning, Southwestern, and Farwestern, require additional manipulations to obtain a cDNA clone. Clearly, the interactive cloning system of choice will depend on the proteins under investigation.

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Authors and Affiliations

  1. Department of Medicine, The University of North Carolina at Chapel Hill, 27599-7280, Chapel Hill, NC, USA

    S. W. Hunt III

  2. Department of Microbiology and Immunology and the Thurston Arthritis Research Center, The University of North Carolina at Chapel Hill, 27599-7280, Chapel Hill, NC, USA

    S. C. Silver & S. W. Hunt III

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  1. S. C. Silver
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  2. S. W. Hunt III
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Silver, S.C., Hunt, S.W. Techniques for cloning cDNAs encoding interactive transcriptional regulatory proteins. Mol Biol Rep 17, 155–165 (1993). https://doi.org/10.1007/BF00986725

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  • DOI: https://doi.org/10.1007/BF00986725

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