Abstract
Methods are described for the isolation, purification, and culture of bovine mammary epithelial cells. Individual cells were microinjected with a plasmid bearing the sequence for the simian virus 40 early region mutant tsA58 which encodes the thermolabile large T-antigen. After selection in G418-containing medium, surviving colonies were recovered and recloned. One of the clones, designated (E-TC), has been cultured for more than 300 population doublings and exhibits some very unusual properties. From low to middle confluency, E-TC cells exhibit the typical cobblestone morphology of epithelial cells. When E-TC cells remain in culture for 2 weeks, the majority of the cells in a single colony maintain the epithelial cobblestone-like morphology but cells at the periphery of the colony become larger, elongated, and less compact, a morphology more typical of myoepithelial-like cells. Myoepithelial cells, in co-culture, always surround epithelial cells (Zavizion et al., J. Dairy Sci. 75: 3381-3392). In co-culture with the BMM-UV bovine myoepithelial cells, E-TC cells are surrounded by the BMM-UV myoepithelial cells, thus the ET-C cells exhibit epithelial-like behavior. In co-culture with the MAC-T bovine epithelial cells, E-TC cells surround the MAC-T cells, thus the ET-C cells exhibit myoepithelial-like behavior. This suggests that E-TC cells may represent an immortalized bovine mammary stem cell (or precursor) which can exhibit myoepithelial-or epithelial-like properties depending upon certain culture conditions.
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Zavizion, B., Van Duffelen, M., Schaeffer, W. et al. Use of microinjection to generate an immortalized bovine mammary cell line with both epithelial and myoepithelial characteristics. Methods Cell Sci 17, 271–282 (1995). https://doi.org/10.1007/BF00986233
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DOI: https://doi.org/10.1007/BF00986233