Plant Systematics and Evolution

, Volume 208, Issue 1–2, pp 11–23 | Cite as

Further cytogenetic analyses of the Croatian triploid shallot “Ljutika” (Allium cepa var.viviparum, Alliaceae) and its comparison with the Indian triploid “Pran”

  • Jasna Puizina
  • Dražena Papeš


Feulgen and silver-stained karyotypes and meiosis of two triploid viviparous onion forms (Allium cepa var.viviparum), the Croatian “Ljutika” and the Indian “Pran”, were comparatively analyzed. The results of chromosome measurements show that “Ljutika” and “Pran” are karyologically not identical, although significant similarities were found in the morphology of their chromosomes. Five geographically distant clones of “Ljutika” showed good agreement in the number and gross morphology of the chromosomes and in the number and position of NORs and interphase nucleoli. Heterotrivalents were predominant in meiosis of “Ljutika” but a relatively high frequency of higher multivalents together with univalents and bivalents were also observed. The relationship between “Ljutika” and “Pran” and their possible origin are discussed.

Key words

Alliaceae Allium cepa A. fistulosum A. cepa var.viviparum Viviparous onions hybridogenic onions triploids karyotypes meiosis nucleolus organizing region 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Havey, M. J., 1991: Molecular characterization of the interspecific origin of viviparous onion. — J. Heredity82: 501–503.Google Scholar
  2. Howell, W., Black, D. A., 1980: Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. — Experientia36: 1014–1015.Google Scholar
  3. Jones, H. A., Mann, L. K., 1963: Onions and their allies. — New York: Hill.Google Scholar
  4. Konvička, O., Levan, L., 1972: Chromosome studies inAllium sativum. — Hereditas72: 129–148.Google Scholar
  5. Koul, A. K., Gohil, R. N., 1971: Further studies on natural triploidy in viviparous onions. — Cytologia36: 253–261.Google Scholar
  6. Langer, A., Koul, A. K., 1983: Studies on nucleolus and nucleolar chromosomes in angiosperms VII. Nature of nucleolar chromosome polymorphism inAllium cepa var.viviparum (Metzg.)Alef. — Cytologia48: 323–332.Google Scholar
  7. Levan, A., Fredga, K., Sandberg, A., 1964: Nomenclature for centromere position in chromosomes. — Hereditas52: 201–220.Google Scholar
  8. McCollum, G. D., 1974: Hybrid origin of top onion,Allium cepa var.viviparum. — Z. Pflanzenzücht.71: 222–232.Google Scholar
  9. Puizina, J., 1992: Cytogenetic evidences of hybrid origin of diploid and triploid cultivars of shallots (A. cepa var.viviparum) from Dalmatia. — M.Sc. Thesis, University of Zagreb.Google Scholar
  10. —, 1995: Highly conservative caryotype of triploid shallot (Allium cepa var.viviparum) from Croatia. — Chrom. Res.3, Suppl.1: 58.Google Scholar
  11. —, 1996: Cytogenetical evidences for hybrid structure and origin of diploid and triploid and triploid shallots (Allium cepa var.viviparum, Liliaceae) from Dalmatia (Croatia). — Pl. Syst. Evol.199: 203–215.Google Scholar
  12. Schubert, I., 1984: Mobile nucleolus organizing regions (NORs) inAllium (Liliaceae s. lat.). — Inferences from the specificity of silver staining. — Pl. Syst. Evol.144: 291–305.Google Scholar
  13. —, 1985: In situ hybridization confirms jumping nucleolus organizing regions inAllium. — Chromosoma2: 143–148.Google Scholar
  14. Singh, F., Ved Brat, S., Khoshoo, T. N., 1967: Natural triploidy in viviparous onions. — Cytologia32: 403–407.Google Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Jasna Puizina
    • 1
  • Dražena Papeš
    • 2
  1. 1.Department of Biology, Faculty of ScienceUniversity of SplitSplitCroatia
  2. 2.Department of Molecular Biology, Faculty of ScienceUniversity of ZagrebZagrebCroatia

Personalised recommendations