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Plant Systematics and Evolution

, Volume 212, Issue 1–2, pp 135–141 | Cite as

Genome size variation inCajanus cajan (Fabaceae): A reconsideration

  • J. Greilhuber
  • R. Obermayer
Article

Abstract

A recent investigation of genome size in certain samples of the pigeonpea,Cajanus cajan, indicates values from 1.55 pg to 1.99 pg (1C level), which is 1.29-fold variation between accessions. In the present analysis those of these accessions which had particularly high or low DNA contents in that study were subjected to a reanalysis using propidium iodide and DAPI flow cytometry and Feulgen densitometry. Only minor differences in genome size, not more than 1.047-fold, were found with flow cytometry, and no significant differences were obtained with Feulgen densitometry. The previously reported genome size cannot be confirmed. It is about half as large and was determined in the present study as 0.825 pg (1C, propidium iodide flow cytometry,Glycine max as standard) and 0.853 pg (1C, Feulgen densitometry,Allium cepa andPisum sativum as standards), respectively.

Key words

Fabaceae Cajanus cajan Genome size variation flow cytometry propidium iodide DAPI Feulgen densitometry 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • J. Greilhuber
    • 1
  • R. Obermayer
    • 1
  1. 1.Institute of BotanyUniversity of ViennaWienAustria

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