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Plant Systematics and Evolution

, Volume 200, Issue 3–4, pp 233–252 | Cite as

Isoenzyme diversity and phylogenetic affinities among thePhaseolus beans (Fabaceae)

  • Vello Jaaska
Article

Abstract

Polyacrylamide gel electrophoretic study of 8 isoenzyme systems encoded by 16 putative gene loci in 23 species of American beans of the genusPhaseolus s. str. and in the Asian moth beanVigna aconitifolia revealed in total 98 allozymes, including 34 taxon-specific and unique, 14 rare-unique and 50 shared allozymes. —P. xanthotrichus var.xanthotrichus and var.zimapanensis differed in allozymes of AAT-A, AAT-D and ADH-C.P. xanthotrichus var.zimapanensis andP. hintonii shared same allozymes of AAT-A and AAT-D, but differed in allozymes of ADH-A and ADH-C. It is proposed to recognizeP. xanthotrichus var.zimapanensis in species rank asP. zimapanensis. — P. acutifolius var.acutifolius and var.tenuifolius, except one accession of the latter, differed in allozymes of AAT-D, ADH-C and FDH-A. — Cladistic analysis of the allozymic data as unordered absence-presence characters disclosed in the genus two major monophyletic species clusters: (1)P. polystachyus, P. ritensis, P. maculatus, P. marechalii, P. jaliscanus, P. salicifolius, P. lunatus, P. filiformis, P. angustissimus, P. acutifolius, P. coccineus, P. vulgaris, P. parvulus, P. pauciflorus, andP. pluriflorus; (2)P. grayanus, P. neglectus, P. pedicellatus, P. microcarpus, P. hintonii, andP. zimapanensis. P. xanthotrichus s. str. andP. zimapanensis are discriminated as paraphyletic, supporting their specific delimitation. — Phenetic analysis of the allozyme data with the UPGMA clustering revealed essentially the same pattern of genetic affinities between the species and additionally clarified the extent of allozymic divergence by taking into account species-specific and unique allozymes.

Key words

Fabaceae Phaseolus Bean species isoenzymes allozyme diversity cladistic and phenetic analysis phylogenetic affinities genetic divergence 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Vello Jaaska
    • 1
  1. 1.Department of BotanyInstitute of Zoology and BotanyTartuEstonia

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