Plant Systematics and Evolution

, Volume 200, Issue 3–4, pp 233–252 | Cite as

Isoenzyme diversity and phylogenetic affinities among thePhaseolus beans (Fabaceae)

  • Vello Jaaska


Polyacrylamide gel electrophoretic study of 8 isoenzyme systems encoded by 16 putative gene loci in 23 species of American beans of the genusPhaseolus s. str. and in the Asian moth beanVigna aconitifolia revealed in total 98 allozymes, including 34 taxon-specific and unique, 14 rare-unique and 50 shared allozymes. —P. xanthotrichus var.xanthotrichus and var.zimapanensis differed in allozymes of AAT-A, AAT-D and ADH-C.P. xanthotrichus var.zimapanensis andP. hintonii shared same allozymes of AAT-A and AAT-D, but differed in allozymes of ADH-A and ADH-C. It is proposed to recognizeP. xanthotrichus var.zimapanensis in species rank asP. zimapanensis. — P. acutifolius var.acutifolius and var.tenuifolius, except one accession of the latter, differed in allozymes of AAT-D, ADH-C and FDH-A. — Cladistic analysis of the allozymic data as unordered absence-presence characters disclosed in the genus two major monophyletic species clusters: (1)P. polystachyus, P. ritensis, P. maculatus, P. marechalii, P. jaliscanus, P. salicifolius, P. lunatus, P. filiformis, P. angustissimus, P. acutifolius, P. coccineus, P. vulgaris, P. parvulus, P. pauciflorus, andP. pluriflorus; (2)P. grayanus, P. neglectus, P. pedicellatus, P. microcarpus, P. hintonii, andP. zimapanensis. P. xanthotrichus s. str. andP. zimapanensis are discriminated as paraphyletic, supporting their specific delimitation. — Phenetic analysis of the allozyme data with the UPGMA clustering revealed essentially the same pattern of genetic affinities between the species and additionally clarified the extent of allozymic divergence by taking into account species-specific and unique allozymes.

Key words

Fabaceae Phaseolus Bean species isoenzymes allozyme diversity cladistic and phenetic analysis phylogenetic affinities genetic divergence 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bassiri, A., Adams, M. W., 1978a: Evaluation of common bean cultivar relationships by means of isozyme electrophoretic patterns. — Euphytica27: 707–720.Google Scholar
  2. —, 1978b: An electrophoretic survey of seedling isozymes in severalPhaseolus species. — Euphytica27: 447–459.Google Scholar
  3. Delgado Salinas, A., 1985: Systematics of the genusPhaseolus (Leguminosae) in North and Central America. — PhD. Thesis, University of Texas, Austin.Google Scholar
  4. Driedger, D. R., Watts, B. M., Hussain, A., Elias, L. G., 1994: Isoenzyme and cotyledon protein variation for identification of black beans (Phaseolus vulgaris L.) with similar seed morphology. — Euphytica74: 27–34.Google Scholar
  5. Escalante, A. M., Coello, G., Eguiarte, L. E., Piñero, D., 1994: Genetic structure and mating systems in wild and cultivated populations ofPhaseolus coccineus andP. vulgaris (Fabaceae). — Amer. J. Bot.81: 1096–1103.Google Scholar
  6. Garvin, D. F., Weeden, N. F., 1994: Isozyme evidence supporting a single geographic origin for domesticated tepary bean. — Crop Sci.34: 1390–1395.Google Scholar
  7. —, 1989: Isoenzyme genetics and linkage in tepary bean,Phaseolus acutifolius A. Gray. — J. Heredity90: 373–376.Google Scholar
  8. Gepts, P., 1990: Biochemical evidence bearing on the domestication ofPhaseolus (Fabaceae) beans. — Econ. Bot.44: 28–38.Google Scholar
  9. Guo, M., Mok, D. W. S., Mok, M. C., 1989: Isozyme banding patterns and embryo development in interspecific crosses ofPhaseolus. — J. Heredity80: 29–32.Google Scholar
  10. Jaaska, V., Jaaska, V., 1988: Isoenzyme variation in the generaPhaseolus andVigna (Fabaceae) in relation to their systematics: aspartate aminotransferase and superoxide dismutase. — Pl. Syst. Evol.159: 145–159.Google Scholar
  11. —, —, 1989: Isoenzyme differentiation between Asian beansVigna radiata andV. mungo. — Biochem. Physiol. Pfl.185: 41–53.Google Scholar
  12. —, 1990: Isoenzyme variation in Asian beans. — Bot. Acta103: 281–290.Google Scholar
  13. Koenig, R., Gepts, P., 1989: Allozyme diversity in wildPhaseolus vulgaris: further evidence for two major centers of genetic diversity. — Theor. Appl. Genet.78: 809–817.Google Scholar
  14. Loulakakis, K. A., Roubelakis-Angelakis, K. A., 1991: Plant NAD (H) — glutamate dehydrogenase consists of two subunit polypeptides and their participation in the seven isoenzymes occurs in ordered ratio. — Pl. Physiol.97: 104–111.Google Scholar
  15. Maréchal, R., Mascherpa, J., Stanier, F., 1978: Étude taxonomique d'un groupe complexe d'espèces des generesPhaseolus etVigna (Papilionaceae) sur la base de données morphologiques et polliniques, traitées par l'analyse informatique. — Boissiera28: 1–273.Google Scholar
  16. Marshall, D. R., Broue, P., Oram, R. N., 1974: Genetic control of alcohol dehydrogenase in narrow-leafed lupins. — J. Heredity65: 198–203.Google Scholar
  17. Piñero, D., Eguiarte, L., 1988: The origin and biosystematic status ofPhaseolus coccineus ssp.polyanthus: electrophoretic evidence. — Euphytica37: 199–203.Google Scholar
  18. Piper, C. V., 1926: Studies in AmericanPhaseolinae. — Contrib. U.S. Natl. Herb.22: 663–701.Google Scholar
  19. Schinkel, C., Gepts, P., 1989: Allozyme variability in the tepary bean,Phaseolus acutifolius A. Gray. — Pl. Breed.102: 182–195.Google Scholar
  20. Singh, S.P., Gepts, P., Debouck, D. G., 1991a: Races of common bean (Phaseolus vulgaris, Fabaceae). — Econ. Bot.45: 379–396.Google Scholar
  21. —, 1991b: Genetic diversity in cultivated common bean. I. Allozymes. — Crop Sci.31: 19–23.Google Scholar
  22. Wall, J. R., Wall, S. W., 1975: Isoenzyme polymorphism in the study of evolution in thePhaseolus vulgaris — P. coccineus complex in Mexico. — InMarkert, C. L., (Ed.): Isoenzymes,4, pp. 289–305. — New York: Academic Press.Google Scholar
  23. Wendel, J. F., Weeden, N. F., 1989: Visualization and interpretation of plant isozymes. — InSoltis, D. E., Soltis, P. S., (Eds): Isozymes in plant biology, pp. 5–45. — Portland, Oregon: Dioscoroides Press.Google Scholar
  24. Weeden, N. F., 1984: Distinguishing among white-seeded bean cultivars by means of allozyme genotypes. — Euphytica33: 199–208.Google Scholar
  25. West, N. B., Garber, E. D., 1967a: Genetic studies of variant enzymes. I. An electrophoretic survey of esterases and leucine aminopeptidases in the genusPhaseolus. — Canad. J. Genet. Cytol.9: 640–645.Google Scholar
  26. —, 1967b: Genetic studies of variant enzymes. II. The inheritance of esterases and leucine aminopeptidases inPhaseolus vulgaris ×P. coccineus. — Canad. J. Genet. Cytol.9: 646–655.Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Vello Jaaska
    • 1
  1. 1.Department of BotanyInstitute of Zoology and BotanyTartuEstonia

Personalised recommendations