Skip to main content
SpringerLink
Log in

Systematics and randomly amplified polymorphic DNA in the genusLeucaena (Leguminosae, Mimosoideae)

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to examine genomic diversity in taxa of the neotropical legume genusLeucaena. Data were analysed using both similarity- and parsimony-based approaches and the data compared to a parsimonybased analysis of chloroplast DNA restriction fragment length polymorphisms (RFLP). Distance-based methods of RAPD analysis produced groups inconsistent with those identified by RFLP analysis. Parsimony-based analysis of the data produced groupings largely consistent with those identified using RFLPs. The major differences were grouping of the two subspecies ofLeucaena diversifolia (subsp.diversifolia and subsp.stenocarpa) in the RAPD tree, but their separation in the RFLP tree. The value of RAPD data in systematics as a result of these data and our understanding of the molecular basis of RAPDs are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams, R. P., Demeke, T., 1993: Systematic relationships inJuniperus based on random amplified polymorphic DNAs (RAPDs). — Taxon42: 553–571.

    Google Scholar 

  • , 1993: RAPD DNA fingerprints and terpenoids: clue to past migrations ofJuniperus in Arabia and east Africa. — Theor. Appl. Genet.87: 22–26.

    Google Scholar 

  • Arnold, M. L., 1993:Iris nelsonii (Iridaceae): origin and genetic composition of a homoploid hybrid species. — Amer. J. Bot.80: 577–583.

    Google Scholar 

  • Boehm, Loew, R., Haag-Kerwer, A., Luetge, U., Rausch, T., 1993: Evaluation of comparative DNA amplification fingerprinting for rapid species identification within the genusClusia. — Bot. Acta106: 448–453.

    Google Scholar 

  • Bremer, B., 1991: Restriction data from chloroplast DNA for phylogenetic reconstruction: Is there only one accurate way of scoring? — Pl. Syst. Evol.175: 39–54.

    Google Scholar 

  • Bremer, K., 1988: The limits of amino acid sequence data in angiosperm phylogenetic reconstruction. — Evolution42: 795–803.

    Google Scholar 

  • Buth, D. G., 1984: The applications of electrophoretic data in systematic studies. — Annu. Rev. Ecol. Syst.15: 501–522.

    Google Scholar 

  • Caetano-Anollés, G., 1993: Amplifying DNA with arbitrary oligonucleotide primers. — PCR Methods Applications3: 85–94.

    Google Scholar 

  • , 1991: DNA amplification fingerprinting using very short arbitrary primers. — Bio/technology9: 553–557.

    Google Scholar 

  • , 1992: Primer-template interactions during DNA amplification fingerprinting with single arbitrary oligonucleotides. — Molec. Gen. Genet.235: 157–165.

    Google Scholar 

  • Chalmers, K. J., Newton, A. C., Waugh, R., Wilson, J., Powell, W., 1994: Evaluation of the extent of genetic variation in mahoganies (Meliaceae) using RAPD markers. — Theor. Appl. Genet.89: 504–508.

    Google Scholar 

  • , 1992: Detection of genetic variation between and within populations ofGliricidia sepium andG. maculata using RAPD markers. — Heredity69: 465–472.

    Google Scholar 

  • Crawford, D. J., Brauner, S., Cosner, M. B., Stuessy, T. F., 1993: Use of RAPD markers to document the origin of the intergeneric hybridxMargyracaena skottsbergii (Rosaceae) on the Juan Fernandez Islands. — Amer. J. Bot.80: 89–92.

    Google Scholar 

  • Demeke, T., Adams, R. P., 1994: The use of RAPDs to determine germplasm collection strategies in the African speciesPhytolacca dodecandra (Phytolaccaceae). — InAdams, R. P., Miller, J. S., Golenberg, E. M., Adams, J. E., (Eds): Conservation of plant genes II: utilization of ancient and modern DNA, pp. 131–140. — Missouri: Missouri Botanical Garden.

    Google Scholar 

  • , 1992: Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study inBrassica. — Theor. Appl. Genet.84: 990–994.

    Google Scholar 

  • Devos, K. M., Gale, M. D., 1992: The use of random amplified polymorphic DNA markers in wheat. — Theor. Appl. Genet.84: 567–572.

    Google Scholar 

  • Donoghue, M. J., Olmstead, R. G., Smith, J. F., Palmer, J. D., 1992: Phylogenetic relationships ofDipsacales based onrbcL sequences. — Ann. Missouri Bot. Gard.79: 333–345.

    Google Scholar 

  • Doyle, J. J., 1992: Gene trees and species trees: molecular systematics as one-character taxonomy. — Syst. Bot.17: 144–163.

    Google Scholar 

  • , 1987: A rapid DNA isolation procedure for small amounts of fresh leaf tissue. — Phytochem. Bull.19: 11–15.

    Google Scholar 

  • Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.

    Google Scholar 

  • , 1988: The detection of phylogeny. — InHawksworth, D. L., (Ed.): Prospects in systematics, pp. 112–127. — Oxford: Clarendon Press.

    Google Scholar 

  • Fitch, W. M., Margoliash, E., 1967: Construction of phylogenetic trees. — Science155: 279–284.

    Google Scholar 

  • Hadrys, H., Balick, M., Schierwater, B., 1992: Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. — Molec. Ecol.1: 55–63.

    Google Scholar 

  • Harris, S. A., Chamberlain, J. R., Hughes, C. E., 1995: New insights into the evolution ofLeucaena Benth. — InPolhill, R. M., Pickersgill, B., (Eds): Advances in legume systematics. — Kew, Royal Botanic Gardens (in press).

    Google Scholar 

  • , 1994: A phylogenetic analysis ofLeucaena (Leguminosae: Mimosoideae). — Pl. Syst. Evol.191: 1–26.

    Google Scholar 

  • Heun, M., Helentjaris, T., 1993: Inheritance of RAPDs in F1 hybrids of corn. — Theor. Appl. Genet.85: 961–968.

    Google Scholar 

  • Hillis, D. M., Dixon, M. T., 1989: Vertebrate phylogeny: evidence from 28 S ribosomal DNA sequences. — InFernholm, B., Bremer, K., Jörnvell, H., (Eds): The hierarchy of life, pp. 355–367. — Amsterdam: Elsevier.

    Google Scholar 

  • Hughes, C. E., 1993:Leucaena genetic resources: the OFILeucaena seed collections and a synopsis of species characteristics. — Oxford: Oxford Forestry Institute.

    Google Scholar 

  • Jaccard, P., 1908: Nouvelles recherches sur la distribution florale. — Bull. Soc. Vaud. Sci. Nat.44: 223–270.

    Google Scholar 

  • Kazan, K., Manners, J. M., Cameron, D. F., 1993a: Genetic relationships and variation in theStylosanthes guianensis species complex assessed by random amplified polymorphic DNA. — Genome36: 36–49.

    Google Scholar 

  • , 1993b: Genetic variation in agronomically important species ofStylosanthes determined using random amplified polymorphic DNA markers. — Theor. Appl. Genet.85: 882–888.

    Google Scholar 

  • Keil, M., Griffin, A. R., 1994: Use of randomly amplified polymorphic DNA (RAPD) markers in the discrimination and verification of genotypes inEucalyptus. — Theor. Appl. Genet.89: 442–450.

    Google Scholar 

  • Koller, B., Lehmann, A., McDermott, J. M., Gessler, C., 1993: Identification of apple cultivars using RAPD markers. — Theor. Appl. Genet.85: 901–904.

    Google Scholar 

  • Legendre, P., Vaudor, A., 1991: The R package: multidimensional analysis, spatial analysis. — Université de Montréal: Département de sciences biologiques.

  • Lorenz, M. Weihe, A., Börner, T., 1994: DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.). — Theor. Appl. Genet.88: 775–779.

    Google Scholar 

  • Lynch, M., 1990: The similarity index and DNA fingerprinting. — Molec. Biol. Evol.7: 478–484.

    Google Scholar 

  • Marsolais, J. V., Pringle, J. S., White, B. N., 1993: Assessment of random polymorphic DNA (RAPD) as genetic markers for determining the origin of interspecific lilac hybrids. — Taxon42: 531–537.

    Google Scholar 

  • Megnegneau, B., Debets, F., Hoekstra, R. F., 1993: Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNA. — Curr. Genet.23: 323–329.

    Google Scholar 

  • Mickevich, M. H., Johnson, M. S., 1976: Congruence between morphological and allozyme data in evolutionary inference and character evolution. — Syst. Zool.25: 260–270.

    Google Scholar 

  • Newbury, H. J., Ford-Lloyd, B. V., 1993: The use of RAPD for assessing variation in plants. — Plant Growth Regul.12: 43–51.

    Google Scholar 

  • Palmer, J. D., 1987: Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. — Amer. Naturalist130: 6–29.

    Google Scholar 

  • Russell, J. R., Hosein, F., Johnson, E., Waugh, R., Powell, W., 1993: Genetic differentiation of cocoa (Theobroma cacao L.) populations revealed by RAPD analysis. — Molec. Ecol.2: 89–97.

    Google Scholar 

  • Saitou, N., Nei, M., 1987: The neighbor-joining method: a new method for reconstructing phylogenetic trees. — Molec. Biol. Evol.4: 406–425.

    Google Scholar 

  • Smith, J. J., Scott-Craig, J. S., Leadbetter, J. R., Bush, G. L., Roberts, D. L., Fulbright, D. W., 1994: Characterization of random amplified polymorphic DNA (RAPD) products fromXanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis. — Molec. Phylogen. Evol.3: 135–145.

    Google Scholar 

  • Sorensson, C. T., Brewbaker, J. L., 1994: Interspecific compatibility among 15Leucaena species (Leguminosae: Mimosoideae) via artificial hybridisations. — Amer. J. Bot.81: 240–247.

    Google Scholar 

  • Stammers, M., Harris, J., Evans, G. M., Hayward, M. D., Forster, J. W., 1995: Use of random PCR (RAPD) technology to analyse hylogenetic relationships in theLolium/Festuca complex. — Heredity74: 19–27.

    Google Scholar 

  • Swofford, D. L., 1993: PAUP: phylogenetic analysis using parsimony, version 3.1 user manual. — Illinois: Illinois Natural History Survey.

    Google Scholar 

  • , 1990: Phylogeny construction. — InHillis, D. M., Moritz, C., (Eds): Molecular systematics, pp. 411–501. — Sunderland, Mass.: Sinauer.

    Google Scholar 

  • Tateno, Y., Nei, M., Tajima, F., 1982: Accuracy of estimated phylogenetic trees from molecular data I: distantly related species. — J. Molec. Evol.18: 387–404.

    Google Scholar 

  • Thormann, C. E., Ferreira, M. E., Camargo, L. E. A., Tivang, J. G., Osborn, T. C., 1994: Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. — Theor. Appl. Genet.88: 973–980.

    Google Scholar 

  • van Buren, R., Harper, K. T., Andersen, W. R., Stanton, D. J., Seyoum, S., England, J. L., 1994: Evaluating the relationship of autumn buttercup (Ranunculus acriformis var.aestivalis) to some close congeners using random amplified polymorphic DNA. — Amer. J. Bot.81: 514–519.

    Google Scholar 

  • Venugopal, G., Mohapatra, S., Salo, D., Mohapatra, S., 1993: Multiple mismatch annealing: basis for random amplified polymorphic DNA fingerprinting. — Biochem. Biophys. Res. Comm.197: 1382–1387.

    Google Scholar 

  • Vierling, R. A., Nguyen, H. T., 1993: Use of RAPD markers to determine the genetic diversity of diploid wheat genotypes. — Theor. Appl. Genet.84: 835–838.

    Google Scholar 

  • Welsh, J., McCelland, M., 1990: Fingerprinting genomes using PCR with arbitrary primers. — Nucleic Acids Res.18: 7213–7218.

    Google Scholar 

  • Wilde, J., Waugh, R., Powell, W., 1992: Genetic fingerprinting ofTheobroma clones using randomly amplified polymorphic DNA markers. — Theor. Appl. Genet.83: 871–877.

    Google Scholar 

  • Williams, C. E., St. Clair, D. A., 1993: Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions ofLycopersicon esculentum. — Genome36: 619–630.

    Google Scholar 

  • Williams, J. G. K., Hanafey, M. K., Rafalski, J. A., Tingey, S. V., 1993: Genetic analysis using random amplified polymorphic DNA markers. — Methods Enzymol.218: 704–740.

    Google Scholar 

  • , 1990: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucleic Acids Res.18: 6531–6535.

    Google Scholar 

  • Yang, X., Quiros, C., 1993: Identification and classification of celery cultivars with RAPD markers. — Theor. Appl. Genet.86: 205–212.

    Google Scholar 

  • Zarate, S. P., 1984: Taxonomic revision of the genusLeucaena Benth. from Mexico. — Bull. Groupe Int. Estude Mimos.12: 24–34.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Oxford Forestry Institute, Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, Oxford, England

    Stephen A. Harris

Authors
  1. Stephen A. Harris
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harris, S.A. Systematics and randomly amplified polymorphic DNA in the genusLeucaena (Leguminosae, Mimosoideae). Pl Syst Evol 197, 195–208 (1995). https://doi.org/10.1007/BF00984639

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00984639

Key words

Search

Navigation