Abstract
Eight species ofAllium subgen.Allium sect.Allium have been studied at the cytological level by means of karyological analyses and at the biochemical level with regard to the proportions of ribosomal DNA. All the species have a basic genome of x = 8.A. sativum, A. commutatum, A. ampeloprasum, andA. vineale possess approximately 0.050% rDNA and two nucleolus organizer regions per basic chromosome set.A. sphaerocephalon andA. arvense have two nucleolus organizers, andA. amethystinum three nucleolus organizers per haploid (n = x) genome: the three species possess approximately 0.075% rDNA.A. acutiflorum has five nucleolus organizer regions per haploid genome and 0.121% rDNA. An attempt to relate these differences with functional and ecological characteristics indicates that evolutionary variation of rDNA proportions is not casual. Such data also can help to define systematic affinities and circumscribe infrageneric taxa.
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References
Amaldi, F., Lava-Sanchez, P. A., andBuongiorno-Nardelli, M., 1973: Nuclear DNA content variability inXenopus laevis: A redundancy regulation common to all gene families. Nature242, 615–617.
Battaglia, E., 1963: Mutazione cromosomica e cariotipi fondamentali inAllium sativum L. Caryologia16, 1–46.
—, andMaggini, F., 1968: The use of osmium for staining the nucleolus in squash technique. Caryologia21, 287–292.
Birnstiel, M., 1967: The nucleolus in cell metabolism. Ann. Rev. Plant Physiol.18, 25–28.
Buongiorno-Nardelli, M., Amaldi, F., andLava-Sanchez, P. A., 1972: Amplification as a rectification mechanism for the redundant rRNA genes. Nature New Biol.238, 134–137.
Bothmer, R. von, 1970: Cytological studies inAllium I. Chromosome numbers and morphology in sect.Allium from Greece. Bot. Notiser123, 519–551.
—, 1972: Four species ofAllium sect.Allium in Greece. Bot. Notiser125, 62–76.
—, 1974a: Studies in the Aegean Flora XXI. Biosystematic studies in theAllium ampeloprasum complex. Opera Bot. (Lund)34, 1–104.
—, 1974b: Karyotype ofAllium chamaespathum Boiss. Bot. Notiser127, 546–547.
—, 1975a: Karyotype variation inAllium bourgeaui. Hereditas79, 125–132.
—, 1975b: TheAllium ampeloprasum complex on Crete. Mitt. Bot. München12, 267–288.
Cullis, C. A., andDavies, D. R., 1975: Ribosomal DNA amounts inPisum sativum. Genetics81, 485–492.
Garbari, F., 1975: The genusAllium in Italy. V.Allium subgen.Chamaeprason (F. Hermann) stat. nov. Taxon24, 541–542.
—, andCela-Renzoni, G., 1974: Il genereAllium in Italia. VII. Il caso diAllium commutatum Guss. Lav. Soc. Ital. Biogeogr., n.s.,5, 3–16.
—, andSenatori, E., 1975: Il genereAllium in Italia. VI. Contributo alla citosistematica di alcune specie. Atti Soc. Tosc. Sc. Nat., Mem.B, 82, 1–23.
Gillespie, D., andSpiegelman, S., 1965: A quantitative assay for DNA/RNA hybrids with DNA immobilized on a membrane. J. Molec. Biol.12, 829–842.
Goldberg, R. B., Bemis, W. P., andSiegel, A., 1972: Nucleic acid hybridization studies within the genusCucurbita. Genetics72, 253–266.
Greuter, W., 1974: Note sur deux espèces nouvelles pour la Corse. Bull. Soc. Bot. Fr.121, 145–147.
Kavenoff, R., andZimm, B. H., 1973: Chromosome-sized DNA molecules fromDrosophila. Chromosoma41, 1–27.
Kollmann, F., 1971:Allium “descendens” in the Mediterranean. Notes Roy. Bot. Edinburgh31, 119–124.
Konvicka, O., andLevan, A., 1972: Chromosomes studies inAllium sativum. Hereditas72, 129–148.
Maggini, F., 1975: Homologies of ribosomal RNA nucleotide sequences in monocots. J. Mol. Evol.4, 317–322.
—, andDe Dominics, R. I., 1975: Correlazione tra lunghezza delle costrizioni secondarie nucleolari e quantità di DNA ribosomale nel genereBellevalia (Liliaceae). Atti A.G.I.20, 204–206.
—, andSalvi, G., 1976: Similarities among ribosomal RNA's ofAngiospermae andGymnospermae. J. Mol. Evol.8, 329–335.
— - 1977: The ribosomal RNA gene number and the length of the nucleolar secondary constrictions inBellevalia romana andB. dubia (Liliaceae): a possible correlation. Caryologia (in press).
—, 1977: Variazioni del contenuto di DNA ribosomale inAllium cepa. Atti A.G.I.22, 183–185.
Mandell, S. D., andHerschy, A. D., 1960: A fractionating column for analysis of nucleic acids. Anal. Biochem.1, 66–77.
Miller, L., andBrown, D. D., 1969: Variation in the activity of nucleolar organzers and their ribosomal gene content. Chromosoma28, 430–444.
Orsino, F., 1975: Flora e vegetazione delle isole Gallinara e Bergeggi (Liguria occidentale). Webbia29, 595–644.
Phillips, R. L., Kleese, R. A., andWang, S. S., 1971: The nucleolus organizer region of maize (Zea mays L.): chromosomal site of DNA complementary to ribosomal RNA. Chromosoma36, 79–88.
—, andWang, S. S., 1974: The nucleolus organizer region of maize (Zea mays L.): Tests for ribosomal gene compensation or magnification. Genetics77, 285–297.
Ramirez, S. A., andSinclair, J. H., 1975: Intraspecific variation of ribosomal gene redundancy inZea mays. Genetics80, 495–504.
Ritossa, F. M., andSpiegelman, S., 1965: Localization of DNA complementary to ribosomal RNA in the nucleolus organizing region ofDrosophila melanogaster. Proc. Nat. Acad. Sci. (Wash.)53, 737–745.
Sinclair, J. H., andBrown, D. D., 1971: Comparative studies of genes for ribosomal RNA in eukaryotes. Biochemistry10, 2761–2769.
—, andHumphrey, R. R., 1974: Variation in rDNA redundancy level and NOR length in normal and variant lines of the Mexican axolotl. J. Cell Science15, 239–257.
Wallace, H., andBirnstiel, M. L., 1966: Ribosomal cistrons and the nucleolar organizer. Biochem. Biophys. Acta (Amst.)114, 296–310.
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Maggini, F., Garbari, F. Amounts of ribosomal DNA inAllium (Liliaceae). Pl Syst Evol 128, 201–208 (1977). https://doi.org/10.1007/BF00984556
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DOI: https://doi.org/10.1007/BF00984556