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Karyotype constancy and genome size variation in BulgarianCrepis foetida s. l. (Asteraceae)

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Abstract

Ten populations ofCrepis foetida from Bulgaria belonging to the three subspeciesfoetida, rhoeadifolia, andcommutata were analyzed karyologically using haematoxylin staining, Giemsa C-banding, fluorochrome banding, Ag-NOR staining, Feulgen cytophotometry (scanning densitometry and video-based image analysis), and propidium iodide flow cytometry. The quantitatively-evaluated karyotype structure was similar among all populations, with minor variation in a few intercalary sites only and in the amount of NOR-associated heterochromatin (satellites). In contrast to the karyotypic constancy the genome size ofC. foetida subsp.commutata was about 10% lower than those of the other two subspecies, which had similar genome sizes. The genome size measurements using three different methods resulted in highly correlated data. The genome size difference adds some weight to previous taxonomic opinions treatingC. foetida subsp.commutata at species level, asC. commutata.

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Authors and Affiliations

  1. Institute of Botany, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 23, 1113, Sofia, Bulgaria

    Dessislava Dimitrova & Stefan Kozhuharov

  2. Institute of Botany of the University of Vienna, Rennweg 14, A-1030, Vienna, Austria

    Irma Ebert & Johann Greilhuber

Authors
  1. Dessislava Dimitrova
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  2. Irma Ebert
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  3. Johann Greilhuber
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  4. Stefan Kozhuharov
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Prof. Dr. Stefan Kozhuharov (4 January 1933–24 August 1997).

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Dimitrova, D., Ebert, I., Greilhuber, J. et al. Karyotype constancy and genome size variation in BulgarianCrepis foetida s. l. (Asteraceae). Pl Syst Evol 217, 245–257 (1999). https://doi.org/10.1007/BF00984369

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  • DOI: https://doi.org/10.1007/BF00984369

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