Abstract
The chromosomes ofAdoxa moschatellina (2n = 36, paleo-4x) contain mostly terminal, occasionally intercalary, negatively heteropycnotic cold-induced regions which correspond to all major C-bands including the satellites, as revealed by sequential analysis. Positively C-stained are also centromeres, the dotlike arms of the 7 telocentric chromosome pairs, and some very narrow intercalary bands; their cold-sensitivity is hardly traceable. There exists a fraction of condensed interphase chromatin, at least after chilling, which is virtually not C-banded (possibly condensed euchromatin).
The DNA amount is 14.3 pg (1 C). The heterochromatin content is 13.0%. The thermal melting profile (Tm corresponding to 38.6% GC) does not reveal a particular AT- or GC-rich fraction. Significantly, the heterochromatin respond to the Hy-banding procedure is neutral.
The distribution of cold-sensitive regions in plants was analysed with the “arm-frame method”: Intercalary positions, clearly, are not especially favoured regions. The obvious deficiency at centromeric positions may depend on the action of natural selection against mechanically labile centromeric regions.
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Dedicated to Univ.-Prof. Dr.Lothar Geitler on the occasion of his 80th birthday.
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Greilhuber, J. C-band distribution, DNA content and base composition inAdoxa moschatellina (Adoxaceae), a plant with cold-sensitive chromosome segments. Pl Syst Evol 131, 243–259 (1979). https://doi.org/10.1007/BF00984257
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DOI: https://doi.org/10.1007/BF00984257