Plant Systematics and Evolution

, Volume 193, Issue 1–4, pp 107–114 | Cite as

Characterization and phylogenetic distribution of a chloroplast DNA rearrangement in theBerberidaceae

  • Y. -D. Kim
  • R. K. Jansen


Restriction site maps and a clone bank of chloroplast DNA (cpDNA) ofMahonia higginsae (Munz)Ahrendt (Berberidaceae) were constructed. The size ofMahonia cpDNA was about 167 kb. Precise mapping using gene probes revealed that cpDNA ofM. higginsae has an inverted repeat (IR) 11.5 kb larger than the tobacco IR. The expansion of the IR into the large single copy region has resulted in the duplication of at least ten genes includingpsbB. The phylogenetic distribution of the expanded IR was examined in twenty-five species ofBerberis andMahonia, twenty species representing the fifteen remaining genera of theBerberidaceae, and four species from four allied families. Our survey indicates that only the species of the closely related generaBerberis andMahonia share the 11.5kb expansion of IR. This result supports their close phylogenetic relationship, which has been suggested previously by chromosomal, morphological, and serological data.

Key words

Berberidaceae Berberis Mahonia higginsae Chloroplast DNA (cpDNA) Inverted Repeats (IR) 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ahrendt, L. W. A., 1961:Berberis andMahonia: a taxonomic revision. — J. Linn. Soc. Bot.57: 1–410.Google Scholar
  2. Chapman, M., 1936: Carpel morphology of theBerberidaceae. — Amer. J. Bot.23: 340–348.Google Scholar
  3. DePamphilis, C. W., Palmer, J. D., 1989: Evolution and function of plastid DNA: a review with special reference to nonphotosynthetic plants. — InBoyer, C. D., Shannon, J. C., Hardison, R. C., (Eds): Physiology, biochemistry, and genetics of nongreen plastids, pp. 182–202. — American Society of Plant Physiologists.Google Scholar
  4. Dermen, H., 1931: A study of chromosome number in two genera ofBerberidaceae:Mahonia andBerberis. — J. Arnold Arbor.12: 281–287.Google Scholar
  5. Downie, S. R., Palmer, J. D., 1991: Use of chloroplast DNA rearrangement in reconstructing plant phylogeny. — InSoltis, P. S., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 14–35. — New York: Chapman & Hall.Google Scholar
  6. Doyle, J. J., Doyle, J. L., 1987: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. — Phytochem. Bull.19: 11–15.Google Scholar
  7. Hasebe, M., Iwatsuki, K., 1990: Chloroplast DNA fromAdiantum capillus-veneris L., a fern species (Adiantaceae); clone bank, physical map and unusual gene localization in comparison with angiosperm chloroplast DNA. — Curr. Genet.17: 359–364.Google Scholar
  8. Jensen, U., 1973: The interpretation of comparative serological results: Nobel symposium25. — InBendz, G., Santesson, J., (Eds): Chemistry in botanical classification, pp. 217–227. — New York: Academic Press.Google Scholar
  9. Johansson, J. T., Jansen, R. K., 1993: Chloroplast DNA variation and phylogeny of theRanunculaceae. — Pl. Syst. Evol.187: 29–49.Google Scholar
  10. Lavin, M., Doyle, J. J., Palmer, J. D., 1990: Evolutionary significance of the loss of chloroplast-DNA inverted repeat in theLeguminosae subfamilyPapilionoideae. — Evolution44: 390–402.Google Scholar
  11. Lidholm, J., Szmidt, A. E., Hallgren, J. E., Gustafsson, P., 1990: The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats. — Mol. Gen. Genet.212: 6–10.Google Scholar
  12. Loconte, H., Estes, J. R., 1989: Phylogenetic systematics ofBerberidaceae andRanunculales (Magnoliidae). — Syst. Bot.14: 565–579.Google Scholar
  13. Maniatis, T., Fritsch, E. F., Sambrook, J., 1982: Molecular cloning. — New York: Cold Spring Harbor Laboratory Press.Google Scholar
  14. Meacham, C. A., 1980: Phylogeny of theBerberidaceae with an evaluation of classifications. — Syst. Bot.5: 149–172.Google Scholar
  15. Nowicke, J. W., Skvarla, J. J., 1981: Pollen morphology and phylogenetic relationships of theBerberidaceae. — Smithsonian Contr. Bot.50: 1–30.Google Scholar
  16. Olmstead, R. G., Palmer, J. D., 1992: A chloroplast DNA phylogeny of theSolanaceae: subfamilial relationships and character evolution. — Ann. Missouri Bot. Gard.79: 346–360.Google Scholar
  17. Palmer, J. D., 1986: Isolation and structural analysis of chloroplast DNA. — Methods Enzymol.118: 167–186.Google Scholar
  18. , 1991: Plastid chromosomes: structure and evolution. — InBogorad, L., Vasil, I. K., (Eds): Cell culture and somatic cell genetics in plants,7. The molecular biology of plastids, pp. 5–53. — New York: Academic Press.Google Scholar
  19. , 1986: Conservation of chloroplast genome structure among vascular plants. — Curr. Genet.10: 823–833.Google Scholar
  20. , 1987: Unusual structure of geranium chloroplast DNA: a triple-sized inverted repeat, extensive gene duplications, multiple inversions, and two repeat families. — Proc. Natl. Acad. Sci. USA84: 769–773.Google Scholar
  21. Raubeson, L. A., Jansen, R. K., 1992: A rare chloroplast-DNA structural mutation is shared by all conifers. — Biochem. Syst. Ecol.20: 17–24.Google Scholar
  22. Sastri, R. L. N., 1969: Floral morphology, embryology, and relationships of theBerberidaceae. — Austral. J. Bot.17: 69–79.Google Scholar
  23. Shen, Y., 1954: Phylogeny and wood anatomy ofNandina. — Taiwania5: 85–92.Google Scholar
  24. Strauss, S. H., Palmer, J. D., Howe, G. T., Doerksen, A. H., 1988: Chloroplast genomes of two conifers lack a large inverted repeat and are extensively rearranged. — Proc. Natl. Acad. Sci. USA85: 3898–3902.Google Scholar
  25. Sugiura, M., 1989: The chloroplast chromosomes in land plants. — Annu. Rev. Cell Biol.5: 51–70.Google Scholar
  26. , 1986: Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: mapping of eleven ribosomal protein genes. — Pl. Sci.44: 211–216.Google Scholar
  27. Terabayashi, S., 1978: Studies in morphology and systematics ofBerberidaceae, II: Floral anatomy ofMahonia japonica (Thunb.) DC. andBerberis thunbergii DC. — Acta Phytotax. Geobot.29: 106–118.Google Scholar
  28. , 1985: The comparative floral anatomy and systematics of theBerberidaceae. I. Morphology. — Mem. Fac. Sci. Kyoto Univ., Ser. Biol.10: 73–90.Google Scholar
  29. , 1987: Seedling morphology of theBerberidaceae. — Acta Phytotax. Geobot.38: 63–74.Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Y. -D. Kim
    • 1
  • R. K. Jansen
    • 1
  1. 1.Department of BotanyUniversity of TexasAustinUSA

Personalised recommendations