Plant Systematics and Evolution

, Volume 202, Issue 3–4, pp 211–218 | Cite as

Unknown New Zealand angiosperm assigned toCunoniaceae using sequence of the chloroplastrbcL gene

  • Phil J. Garnock-Jones
  • Gail M. Timmerman
  • Steven J. Wagstaff


We report the first example of identification of an unknown flowering plant to family level using comparison of DNA sequences. The newly-discovered New Zealand plant had not been identified by traditional methods, but has been placed in the Gondwanic familyCunoniaceae on the basis of the sequence of its chloroplastrbcL gene. It has not flowered, but its vegetative characters are typical of the family. However, it is clearly unusual in several morphological and anatomical features and appears to match no known genus. We consider that, although it probably represents an unnamed new genus, it could possibly be a mutant dwarf form ofWeinmannia racemosa, so it is not prudent to name it at this time.

Key words

Angiosperms Cunoniaceae rbcL sequence cladistic analysis identification New Zealand Gondwana 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chase, M. W., Soltis, D. E., Olmstead, R. G., Morgan, D., Les, D. H., Mishler, B. D., Duval, M. R., Price, R. A., Hillis, H. G., Qiu, Y.-L., Kron, K. A., Rettig, J. H., Conti, E., Palmer, J. D., Manhart, J. R., Sytsma, K. J., Michaels, H. J., Kress, W. J., Karol, K. G., Clark, W. D., Hedrén, M., Gaut, B. S., Jansen, R. K., Kim, K.-J., Wimpee, C. F., Smith, J. F., Furnier, G. R., Strauss, S. H., Xiang, Q.-Y., Plunkett, G. M., Soltis, P. S., Swensen, S. M., Williams, S. E., Gadek, P. A., Quinn, C. J., Eguiarte, L. E., Golenberg, E., Learn, G. H., Graham, S. W., Barrett, S. C. H., Dayanandan, S., Albert, V. A., 1993: Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid generbcL. — Ann. Missouri Bot. Gard.80: 528–580.Google Scholar
  2. Dickison, W. C., Rutishauser, R., 1990: Developmental morphology of stipules and systematics of theCunoniaceae and presumed allies. II. Taxa without interpetiolar stipules and conclusions. — Bot. Helv.100: 75–95.Google Scholar
  3. Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.Google Scholar
  4. —, 1978: Cases in which parsimony and compatibility methods will be positively misleading. — Syst. Zool.27: 27–33.Google Scholar
  5. Hennig, W., 1950: Grundzüge einer Theorie der phylogenetischen Systematik. — Berlin: Deutscher Zentralverlag.Google Scholar
  6. Hufford, L., Dickison, W. C., 1992: A phylogenetic analysis ofCunoniaceae. — Syst. Bot.17: 181–200.Google Scholar
  7. Mullis, K. B., 1990: The unusual origin of the polymerase chain reaction. — Sci. Amer.262: 56–65.Google Scholar
  8. Mullis, K., Faloona, F., 1987: Specific synthesis of DNA in vitro via a polymerase catalysed chain reaction. — Meth. Enzymol.55: 335–350.Google Scholar
  9. Nickrent, D. L., Soltis, D. E., 1995: A comparison of angiosperm phylogenies from nuclear 18S rDNA andrbcL sequences. — Ann. Missouri Bot. Gard.82: 208–234.Google Scholar
  10. Olmstead, R. G., Palmer, J. D., 1994: Chloroplast DNA systematics: a review of methods and data analysis. — Amer. J. Bot.81: 1205–1224.Google Scholar
  11. —, 1992: Monophyly of theAsteridae and identification of their major lineages inferred from DNA sequences ofrbcL. — Ann. Missouri Bot. Gard.79: 249–265.Google Scholar
  12. Patel, R. N., 1990: Wood anatomy of the dicotyledons indigenous to New Zealand 20.Cunoniaceae. — New Zealand J. Bot.28: 347–355.Google Scholar
  13. Rutishauser, R., Dickison, W. C., 1989: Developmental morphology of stipules and systematics of theCunoniaceae and presumed allies. I. Taxa with interpetiolar stipules. — Bot. Helv.99: 147–169.Google Scholar
  14. Sambrook, J., Fritsch, E. F., Maniatis, T., 1989: Molecular cloning: a laboratory manual. — New York: Cold Spring Harbor Laboratory.Google Scholar
  15. Sanger, F., Nicklen, S., Coulson, A. R., 1977: DNA sequencing with chain-terminating inhibitors. — Proc. Natl. Acad. Sci. USA74: 5463–5467.Google Scholar
  16. Shaw, W. B., Beadel, S. M., Eckroyd, C. E., 1989: A new plant record from Te Urewera National Park. — New Zealand Bot. Soc. Newsletter15: 12–13.Google Scholar
  17. Swofford, D. L., 1993: PAUP: phylogenetic analysis using parsimony version 3.1.1. — Champaign: Illinois Natural History Survey.Google Scholar
  18. Timmerman, G. M., Frew, T. J., Miller, A. L., Weeden, N. F., Jermyn, W. A., 1993: Linkage mapping of sbm-1, a gene conferring resistance to pea seed-borne mosaic virus, using molecular markers inPisum sativum. — Theor. Appl. Genet.85: 609–615.Google Scholar
  19. Wylie, E. O., 1981: Phylogenetics — the theory and practice of phylogenetic systematics. — New York: Wiley.Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Phil J. Garnock-Jones
    • 1
  • Gail M. Timmerman
    • 2
  • Steven J. Wagstaff
    • 3
  1. 1.School of Biological SciencesVictoria University of WellingtonWellingtonNew Zealand
  2. 2.Institute for Crop and Food ResearchChristchurchNew Zealand
  3. 3.Manaaki Whenua — Landcare ResearchLincolnNew Zealand

Personalised recommendations