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Plant Systematics and Evolution

, Volume 132, Issue 1–2, pp 27–51 | Cite as

Analysis of nucleolus organizer regions (NORs) in mitotic and polytene chromosomes ofPhaseolus coccineus by silver staining and Giemsa C-banding

  • Dieter Schweizer
  • Peter Ambros
Article

Abstract

Chromosomes with active nucleolus organizer regions (NORs) were visualized in root tip metaphases ofPhaseolus coccineus using the silver staining technique. A mean number of 5.5 Ag-NORs per cell was observed in 54 cells from eight plants. In the endopolyploid nuclei of the suspensor the silver technique did not demonstrate the reported specificity for nucleolus organizer activity, because there was usually pale staining of nucleoli and preferential staining of heterochromatic regions in the polytene chromosomes including pericentromeric material, telomeres and NORs. The mean number of NORs per nucleolus as detected by this method was 5.8 (28 nucleoli analysed). Using a modified preparation technique, giant chromosomes stained pale, but nucleoli of suspensor cells displayed darkly silver staining internal domains, each of which originating from a nucleolus organizer.—Giemsa C-banding of endopolyploid suspensor nuclei revealed C-positive nucleolus organizers with darkly staining intranucleolar fibrils. The latter were frequently involved in inter-NOR associations. In 34 nucleoli analysed, the mean number of Giemsa C-positive NORs per nucleolus was 6.0.

Key words

Fabaceae Phaseolus coccineus Silver staining Giemsa C-banding nucleolus organizer regions (NORs) heterochromatin polytene chromosomes suspensor cells 

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References

  1. Avanzi, S., Durante, M., Cionini, P. G., D'Amato, F., 1972: Cytological localization of ribosomal cistrons in polytene chromosomes ofPhaseolus coccineus. — Chromosoma39, 191–203.Google Scholar
  2. Battaglia, E., 1957: A new “5 minutes-fixation” in chromosome analysis. — Caryologia9, 368–370.Google Scholar
  3. Brady, T., 1973: Cytological studies on the suspensor polytene chromosomes ofPhaseolus: DNA content and synthesis and the ribosomal cistrons. — Caryologia (Suppl.)25, 233–259.Google Scholar
  4. , 1972: Cytolocalization of ribosomal cistrons in plant polytene chromosomes. — J. Cell Biol.53, 827–832.Google Scholar
  5. Deumling, B., Nagl, W., 1978: DNA characterization, satellite DNA localization, and nuclear organization inTropaeolum majus. — Cytobiologie16, 412–420.Google Scholar
  6. Durante, M., Cionini, P. G., Avanzi, S., Cremonini, R., D'Amato, F., 1977: Cytological localization of the genes for the four classes of ribosomal RNA (25 S, 18 S, 5.8 S and 5 S) in polytene chromosomes ofPhaseolus coccineus. — Chromosoma60, 269–282.Google Scholar
  7. Geitler, L., 1953: Endomitose und endomitotische Polyploidisierung. — In: Protoplasmatologia/Handbuch der ProtoplasmaforschungVI/V. — Wien: Springer.Google Scholar
  8. Goodpasture, C., Bloom, S. E., 1975: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. — Chromosoma53, 37–50.Google Scholar
  9. Howell, W. M., Denton, T. E., Diamond, J. R., 1975: Differential staining of the satellite regions of human acrocentric chromosomes. — Experientia31, 260–262.Google Scholar
  10. Knälmann, M., Burger, E.-C., 1977: Cytologische Lokalisation von 5 S und 18/25 S RNA Genorten in Mitose-Chromosomen vonVicia faba. — Chromosoma61, 177–192.Google Scholar
  11. Marks, G. E., 1975: The Giemsa-staining centromeres ofNigella damascena. — J. Cell Sci.18, 19–25.Google Scholar
  12. Marks, G. E., Schweizer, D., 1974: Giemsa banding: Karyotype differences in some species ofAnemone and inHepatica nobilis. — Chromosoma44, 405–416.Google Scholar
  13. Martin-De Leon, P. A., Fleming, M. E., Petrosky, D. L., 1978: Patterns of silver staining in cells of six-day blastocyst and kidney fibroblast of the domestic rabbit. — Chromosoma67, 245–252.Google Scholar
  14. Miller, D. A., Dev, V. G., Tantravahi, R., Miller, O. J., 1976: Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. — Exp. Cell Res.101, 235–243.Google Scholar
  15. Miller, O. J., Miller, D. A., Dev, V. G., Tantravahi, R., Croce, C. M., 1976: Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. — Proc. Nat. Acad. Sci. USA73, 4531–4535.Google Scholar
  16. Nagl, W., 1962: 4096-Ploidie und „Riesenchromosomen“ im Suspensor vonPhaseolus coccineus. — Naturwissenschaften49, 261–262.Google Scholar
  17. , 1965: Die SAT-Riesenchromosomen der Kerne des Suspensors vonPhaseolus coccineus und ihr Verhalten während der Endomitose. — Chromosoma16, 511–520.Google Scholar
  18. , 1967: Die Riesenchromosomen vonPhaseolus coccineus L.: Baueigentümlichkeiten, Strukturmodifikationen, zusätzliche Nukleolen und Vergleich mit den mitotischen Chromosomen. — Österr. Bot. Z.114, 171–182.Google Scholar
  19. , 1970: Temperature-dependent functional structures in the polytene chromosomes ofPhaseolus, with special reference to the nucleolus organizers. — J. Cell Sci.6, 87–107.Google Scholar
  20. , 1973: Origin and fate of the micronucleoli in the giant cells of thePhaseolus suspensor. — Nucleus16, 100–109.Google Scholar
  21. , 1974: ThePhaseolus suspensor and its polytene chromosomes. — Z. Pflanzenphysiol.73, 1–44.Google Scholar
  22. , 1978: Endopolyploidy and Polyteny in Differentiation and Evolution. — Amsterdam: North-Holland.Google Scholar
  23. Nardi, I., De Lucchini, S., Barsacchi-Pilone, G., Andronico, F., 1978: Chromosome location of the ribosomal RNA genes inTriturus vulgaris meridionalis (Amphibia, Urodela). — Chromosoma70, 91–99.Google Scholar
  24. Scheuermann, W., Knälmann, M., 1975: Localization of ribosomal cistrons in metaphase chromosomes ofVicia faba (L.). — Exp. Cell Res.90, 463–465.Google Scholar
  25. Schwarzacher, H. G., Mikelsaar, A.-V., Schnedl, W., 1977: Silverstaining of nucleolus organizer regions in man: light- and electronmicroscopic observations. — Chromosomes Today6, 45–50.Google Scholar
  26. Schweizer, D., 1973: Differential staining of plant chromosomes with Giemsa. — Chromosoma40, 307–320.Google Scholar
  27. , 1976a: Giemsa and fluorochrome banding of polytene chromosomes inPhaseolus vulgaris andP. coccineus. — In:Jones, K., Brandham, P. E. (Eds.): Current Chromosome Research, 51–56. — Amsterdam: Elsevier/North-Holland.Google Scholar
  28. , 1976b: Reverse fluorescent chromosome banding with chromomycin and DAPI. — Chromosoma58, 307–324.Google Scholar
  29. , 1972: Nuclear DNA contents ofPisum genotypes grownin vivo andin vitro. — Planta106, 23–29.Google Scholar
  30. , 1976: Giemsa banded karyotypes, systematics, and evolution inAnacyclus (Asteraceae-Anthemideae). — Pl. Syst. Evol.126, 107–148.Google Scholar
  31. Stocker, A. J., Fresquez, C., Lentzios, G., 1978: Banding studies on the polytene chromosomes ofRhynchosciara hollaenderi. — Chromosoma68, 337–356.Google Scholar
  32. Tantravahi, R., Miller, D. A., Dev, V. G., Miller, O. J., 1976: Detection of nucleolus organizer regions in chromosomes of human, chimpanzee, gorilla, orangutan and gibbon. — Chromosoma56, 15–27.Google Scholar
  33. Tschermak-Woess, E., 1957: Über das regelmäßige Auftreten von „Riesenchromosomen“ im Chalazahaustorium vonRhinanthus. — Chromosoma8, 523–544.Google Scholar
  34. , 1967: Der eigenartige Verlauf der I. meiotischen Prophase vonRhinanthus, die Riesenchromosomen und das besondere Verhalten der kurzen Chromosomen in Mitose, Meiose und hochendopolyploiden Kernen. — Caryologia20, 135–152.Google Scholar
  35. , 1971: Endomitose. — In:Altmann, H.-W., & al. (Eds.): Der Zellkern. Handb. Allg. Pathol.2/2, 569–625. — Berlin, Heidelberg, New York: Springer.Google Scholar
  36. , 1963: Das Verhalten von B-Chromosomen besonderer Ausbildung in den endopolyploiden Riesenkernen des chalazalen Endospermhaustoriums vonRhinanthus. — Österr. Bot. Z.110, 468–480.Google Scholar
  37. Varley, J. M., 1977: Patterns of silver staining of human chromosomes. — Chromosoma61, 207–214.Google Scholar
  38. , 1978: Silver staining of the lampbrush chromosomes ofTriturus cristatus carnifex. — Chromosoma67, 233–244.Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Dieter Schweizer
    • 1
  • Peter Ambros
    • 1
  1. 1.Institut für BotanikUniversität WienWienAustria

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