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Detection of 5S rDNA and other repeated DNA on supernumerary B chromosomes ofTriticum species (Poaceae)

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Abstract

The supernumerary B chromosomes ofTriticum speltoides andT. tripsacoides were analyzed in mitotic metaphases of spike primordium cells by C-banding and in situ hybridization (ISH) analyzes. B chromosomes ofT. speltoides have larger telomeric and interstitial C-bands, whereas those ofT. tripsacoides are almost completely devoid of C-bands. A prominent ISH site of rye related DNA sequences (using probe pSc119) was detected on B chromosomes ofT. tripsacoides and only a minor ISH site was observed on theT. speltoides B chromosomes. However, two ISH sites of 5S rRNA loci were detected at a terminal and an interstitial location of theT. speltoides B chromosomes. These sites were absent on B chromosomes ofT. tripsacoides. The results are discussed with respect to the phylogenetic origin of these B chromosomes.

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Friebe, B., Jiang, J. & Gill, B. Detection of 5S rDNA and other repeated DNA on supernumerary B chromosomes ofTriticum species (Poaceae). Pl Syst Evol 196, 131–139 (1995). https://doi.org/10.1007/BF00982954

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  • DOI: https://doi.org/10.1007/BF00982954

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