Abstract
The in-situ inter- and intracellular localization patterns of phosphoenolpyruvate (PEP) and ribulose 1,5-bisphosphate (RuBP) carboxylases in green leaves of severalPanicum species were investigated using an indirect immunofluorescence technique. Four species were examined and compared:P. miliaceum (C4),P. bisulcatum (C3), andP. decipiens andP. milioides (C3−C4 intermediates which have Kranz-like leaf anatomy and reduced photorespiration). In the C4 Panicum, PEP carboxylase was located in the cytosol of the mesophyll cells and RuBP carboxylase was restricted to the bundle-sheath chloroplasts. In contrast, in the C3 Panicum species, PEP carboxylase was found throughout the leaf chlorenchyma, in both the cytosol and chloroplasts, and RuBP carboxylase was located in the chloroplasts. For the C3−C4 intermediate plants, the patterns depended on the species examined. ForP. decipiens, the in-situ localization of both carboxylases was similar to that described forP. bisulcatum and other C3 plants. However, inP. milioides, PEP carboxylase was found exclusively in the cytosol of the mesophyll cells, as inP. miliaceum and other C4 species, whereas RuBP carboxylase was distributed in both the mesophyll and bundle-sheath chloroplasts.
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Abbreviations
- PEP:
-
phosphoenolpyruvate
- RuBP:
-
ribulose 1,5-bisphosphate
References
Brown, R.H., Morgan, J.A. (1980) Photosynthesis of grass species differing in carbon dioxide fixation pathways. VI. Differential effects of temperature and light intensity on photorespiration in C3, C4, and intermediate species. Plant Physiol.66, 541–544
Brown, R.H., Bouton, J.H., Rigsby, L., Rigler, M. (1983) Photosynthesis of grass species differing in carbon dioxide fixation pathways. VIII. Ultrastructural characteristics ofPanicum species in the Laxa group. Plant Physiol.71, 425–431
Edwards, G.E., Ku, M.S.B., Hatch, M.D. (1982) Photosynthesis inPanicum milioides, a species with reduced photorespiration. Plant Cell Physiol.23, 1185–1195
Hall, N.P., Tolbert, N.E. (1978) A rapid procedure for the isolation of ribulose bisphosphate carboxylase/oxygenase from spinach leaves. FEBS Lett.96, 167–169
Hattersley, P.W., Watson, L., Osmond, C.B. (1977) In situ immunofluorescent labelling of ribulose-1,5-bisphosphate carboxylase in leaves of C3 and C4 plants. Aust. J. Plant Physiol.4, 523–539
Holaday, A.S., Black, C.C. (1981) Comparative characterization of phosphoenolpyruvate carboxylase in C3, C4, and C3−C4 intermediatePanicum species. Plant Physiol.67, 330–334
Holaday, A.S., Chollet, R. (1983) Photosynthetic/photorespiratory carbon metabolism in the C3−C4 intermediate species,Moricandia arvensis andPanicum milioides. Plant Physiol.73, 740–745
Johnson, G.D., de C. Nogueira-Araujo, G.M. (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods43, 349–350
Ku, S.B., Edwards, G.E., Kanai, R. (1976) Distribution of enzymes related to C3 and C4 pathway of photosynthesis between mesophyll and bundle sheath cells ofPanicum hians andPanicum milioides. Plant Cell Physiol.17, 615–620
Morgan, J.A., Brown, R.H. (1979) Photosynthesis in grass species differing in carbon dioxide fixation pathways. II. A search for species with intermediate gas exchange and anatomical characteristics. Plant Physiol.64, 257–262
Morgan, J.A., Brown, R.H., Reger, B.J. (1980) Photosynthesis in grass species differing in carbon dioxide fixation pathways. III. Oxygen response and enzyme activities of species in the Laxa group ofPanicum. Plant Physiol.65, 156–159
Perrot-Rechenmann, C., Vidal, J., Maudinas, B., Gadal, P. (1981) Immunocytochemical study of phosphoenolpyruvate carboxylase in nodulatedAlnus glutinosa. Planta153, 14–17
Perrot-Rechenmann, C., Vidal, J., Brulfert, J., Burlet, A., Gadal, P. (1982) A comparative immunocytochemical localization study of phosphoenolpyruvate carboxylase in leaves of higher plants. Planta155, 24–30
Rathnam, C.K.M., Chollet, R. (1980) Photosynthetic carbon metabolism in C4 plants and C3−C4 intermediate species. Progr. Phytochem.6, 1–48
Vidal, J., Godbillon, G., Gadal, P. (1980) Recovery of active, highly purified phosphoenolpyruvate carboxylase from specific immunoadsorbent column. FEBS Lett.118, 31–34
Vidal, J., Godbillon, G., Gadal, P. (1982) Estimation ofSorghum leaf phosphoenolpyruvate carboxylase protein using an immunoadsorbent column. Phytochemistry21, 2829–2830
Wilson, J.R., Hattersley, P.W. (1983) In vitro digestion of bundle sheath cells in rumen fluid and its relation to the suberized lamella and C4 photosynthetic type inPanicum species. Grass Forage Sci.38, 219–223
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Perrot-Rechenmann, C., Chollet, R. & Gadal, P. In-situ immunofluorescent localization of phosphoenolpyruvate and ribulose 1,5-bisphosphate carboxylases in leaves of C3, C4, and C3−C4 intermediatePanicum species. Planta 161, 266–271 (1984). https://doi.org/10.1007/BF00982924
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DOI: https://doi.org/10.1007/BF00982924