Abstract
Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growth (embryonal: isopentenyladenosine, 4 ng g-1 fresh weight [FW]; isopentenyladenine, 1.4 ng g-1 FW; zeatin, 3.6 ng g-1 FW; proembryonal: isopentenyladenosine, 58.3 ng g-1 FW; isopentenyladenine, 7.9 ng g-1 FW; zeatin 11.1 ng g-1 FW). The proembryonic culture medium but not the embryonic culture medium contained isopentenyladenosine up to 28 pg ml-1 during logarithmic growth. No correlation between different embryonic stages and the endogenous cytokinin level was obvious.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- FW:
-
fresh weight
- GC-MS:
-
gas chromatography-mass spectrometry
- HPLC:
-
high-performance liquid chromatography
- RIA:
-
radioimmunoassay
- SIM:
-
single-ion monitoring
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Ernst, D., Oesterhelt, D. & Schäfer, W. Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.). Planta 161, 240–245 (1984). https://doi.org/10.1007/BF00982919
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DOI: https://doi.org/10.1007/BF00982919