Abstract
Phycomyces blakesleeanus colour mutants of a new class have been isolated. The mutants form red mycelia when grown in the dark and show vegetative segregation. The affected gene, amedcarE, behaves as an extranuclear genetic factor. The red phenotype ofcarE mutants is caused by the accumulation of β-carotene in a form showing spectral shift to longer wavelengths. The spectral shift is abolished in vitro by heat or protease treatment and in vivo by the presence of light or retinol.
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Abbreviations
- SIV:
-
glucose-asparagine minimal medium
- Tris:
-
2-amino-2-(hydroxymethyl)-1,3-propanediol
References
Aragón, C.M.G., Murillo, F.J., De la Guardia, M.D., Cerdá-Olmedo, E. (1976) An enzyme complex for the dehydrogenation of phytoene inPhycomyces. Eur. J. Biochem.63, 71–75
Arlett, C.F., Grindle, M., Jinks, J.L. (1962) The “red” cytoplasmic variant ofAspergillus nidulans. Heredity17, 197–209
Bergman, K., Eslava, A.P., Cerdá-Olmedo, E. (1973) Mutants ofPhycomyces with abnormal phototropism. Mol. Gen. Genet.123, 1–16
Cerdá-Olmedo, E., Torres-Martinez, S. (1979) Genetics and regulation of carotene biosynthesis. Pure Appl. Chem.51, 631–637
Davies, B.H. (1965) Analysis of carotenoid pigments. In: Chemistry and biochemistry of plant pigments, pp. 489–532, Goodwin, T.W., ed. Academic Press, New York London
De la Guardia, M.D., Aragón, C.M.G., Murillo, F.J., Cerdá-Olmedo, E. (1971) A carotenogenic enzyme aggregate inPhycomyces: evidence from quantitative complementation. Proc. Natl. Acad. Sci. USA68, 2012–2015
Eslava, A.P., Alvarez, M.I., Cerdá-Olmedo, E. (1974) Regulation of carotene biosynthesis inPhycomyces by vitamin A and β-ionone. Eur. J. Biochem.48, 617–623
Fincham, J.R.S., Day, P.R., Radford, A. (1979) Fungal genetics. Blackwell, Oxford London Edinburgh Melbourne
Garton, G.A., Goodwin, T.W., Lijinsky, W. (1951) General conditions governing β-carotene synthesis by the fungusPhycomyces blakesleeanus Burgeff. Biochem. J.48, 154–163
Heisenberg, M., Cerdá-Olmedo, E. (1968) Segregation of heterokaryons in the asexual cycle ofPhycomyces. Mol. Gen. Genet.102, 187–195
Ji, T.H., Hess, J.L., Benson, A.A. (1968) Studies on chloroplast membrane structure. I. Association of pigments with chloroplast lamellar protein. Biochim. Biophys. Acta150, 676–685
Jinks, J.L. (1963) Cytoplasmic inheritance in fungi. In: Methodology in basic genetics, pp. 325–354, Burdette, W.J., ed. Holden-Day, San Francisco
Ke, B. (1971) Carotenoproteins. Methods Enzymol.23, 624–636
López-Díaz, I., Cerdá-Olmedo, E. (1980) Relationship of photocarotenogenesis to other behavioural and regulatory responses inPhycomyces. Planta150, 134–139
Markwell, M.A.K., Haas, S.M., Bieber, L.L., Tolbert, N.E. (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem.87, 206–210
McEvoy, F.A., Lynn, W.S. (1973) Chloroplast membrane proteins. II. Solubilization of the lipophillic components. J. Biol. Chem.248, 4568–4573
Medina, J.R. (1977) Effect of CPTA on carotenogenesis byPhycomyces carA mutants. Plant Sci. Lett.17, 201–205
Murillo, F.J., Cerdá-Olmedo, E. (1976) Regulation of carotene synthesis inPhycomyces. Mol. Gen. Genet.148, 19–24
Murillo, F.J., Calderón, I.L., López-Díaz, I., Cerdá-Olmedo, E. (1978) Carotene-superproducing strains ofPhycomyces. Appl. Environ. Microbiol.36, 639–642
Murillo, F.J., Torres-Martínez, S., Aragón, C.M.G., Cerdá-Olmedo, E. (1981) Substrate transfer in carotene biosynthesis inPhycomyces. Eur. J. Biochem.119, 511–516
Nishimura, M., Takamatsu, K. (1957) A carotene-protein complex isolated from green leaves. Nature (London)180, 699–700
Ootaki, T., Lighty, A.C., Delbrück, M., Hsu, W.J. (1973) Complementation between mutants ofPhycomyces deficient with respect to carotenogenesis. Mol. Gen. Genet.121, 57–70
Revuelta, J.L. (1981) Fotorregulación de la biosintesis de β-caroteno enPhycomyces blakesleeanus. Tesis Doctoral, Universidad de León, Espana
Roncero, M.I.G. (1980) Genética dePhycomyces blakesleeanus. Tesis Doctoral, Universidad de Sevilla, España
Roncero, M.I.G., Cerdá-Olmedo, E. (1982) Genetics of carotene biosynthesis inPhycomyces. Curr. Genet.5, 5–8
Salares, V.R., Young, N.M., Berstein, H.J., Carey, P.R. (1979) Mechanisms of spectral shifts in lobster carotenoproteins. The resonance Raman spectra of ovoverdin and the crustacianins. Biochim. Biophys. Acta576, 176–191
Sutter, R.P. (1975) Mutations affecting sexual development inPhycomyces blakesleeanus. Proc. Natl. Acad. Sci. USA72, 127–130
Torres-Martinez, S., Murillo, F.J., Cerdá-Olmedo, E. (1980) Genetics of lycopene cyclization and substrate transfer in β-carotene biosynthesis inPhycomyces. Genet. Res.36, 299–309
Vernon, L.P., Ke, B., Hilton, H.M., Shaw, E.R. (1969) Composition and structure of spinach subchloroplast fragments obtained by the action of Triton-X100. Prog. Photosynth. Res.1, 137–148
Wieckowski, S., Wloch, E., Broniowska, B. (1983) Long-wavelength absorbing forms of carotenoids. (Abstr.) Sixth Int. Congr. Photosynt., Brussels, p. 416
Zagalsky, P.F. (1976) Carotenoid-protein complexes. Pure Appl. Chem.47, 103–120
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De la Concha, A., Murillo, F.J. Accumulation of a complex form of β-carotene byPhycomyces blakesleeanus cytoplasmic mutants. Planta 161, 233–239 (1984). https://doi.org/10.1007/BF00982918
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DOI: https://doi.org/10.1007/BF00982918