Planta

, Volume 161, Issue 3, pp 207–215 | Cite as

Seasonal variations in the polar-transport pathways and retention sites of [3H]indole-3-acetic acid in young branches ofFagus sylvatica L.

  • S. Lachaud
  • J. L. Bonnemain
Article

Abstract

Branches were cut from young beeches (Fagus sylvatica L.) at various stages of the annual cycle and [3H]indole-3-acetic acid (0.35 nmol) was applied to the whole surface of the apical section of each branch, just below the apical bud. The labelled pulse (moving auxin) and the following weakly radioactive zone (auxin and metabolites retained by the tissues) were localized by counting: microautoradiographss were made using cross sections from these two regions. During the second fortnight of April, auxin was transported by nearly all the cells of the young primary shoot, but the label was more concentrated in the vascular bundles. Auxin transport became the more localized: the cortical parenchyma appeared to lose its ability to transport the hormone (end of April), followed in turn by the pith parenchyma (May). Polar auxin movement at that time was limited to the outer part of the bundle (cambial zone and phloem) and to the inner part (protoxylem parenchyma). Later protoxylem parenchyma ceased to carry auxin. During the whole period of cambial activity, auxin was transported and retained mainly by the cambial zone and its recent derivatives. In September, before the onset of dormancy, and in February, at the end of the resting period, the transport pathways and retention sites for auxin were mainly in the phloem, where sieve tubes often completely lacked radiolabel. When cambial reactivation occurred in the one-year shoot, auxin was mainly carried and retained again in the cambial zone and differentiating derivatives.

Key words

Auxin (retention transport) Cambium Fagus auxin 

Abbreviation

IAA

indole-3-acetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bonga, J.M., Clark, J. (1963) Chromatographic detection of seasonal changes in the location of indole-3-acetic acid in balsam fir. Can. J. Bot.41, 1133–1134Google Scholar
  2. Bonnemain, J.L. (1971) Transport et distribution des traceurs après application de AIA-2-C14 sur les feuilles deVicia faba. C.R. Acad. Sci. Ser. D.273, 1699–1702Google Scholar
  3. Bonnemain, J.L. (1972) La plante supérieure, siège d'une circulation complexe. Physiol Vég.10, 697–729Google Scholar
  4. Bonnemain, J.L. (1978) Some aspects of the physiology of collateral and bicollateral bundles. In: Inaugural meeting, F. E. S. P. P., Edinburgh, Symp.: Transport in plants, pp. 11–12. Edinburgh University, EdinburghGoogle Scholar
  5. Bonnemain, J.L. (1980) Microautoradiography as a tool for the recognition of phloem transport. Ber. Dtsch. Bot. Ges.93, 99–107Google Scholar
  6. Bourbouloux, A., Bonnemain, J.L. (1973) Transport de l'auxine-14C en provenance de jeunes gousses deVicia faba L. Planta115, 161–172Google Scholar
  7. Bourbouloux, A., Bonnemain, J.L. (1974) Transport, distribution et métabolisme de l'auxine dans la racine deVicia faba L., après application de [C14]AIA ou de[H3]AIA sur le bourgeon. Planta119, 169–182Google Scholar
  8. Bourbouloux, A., Bonnemain, J.L. (1979) The different components of the movement and the areas of retention of labelled molecules after application of [3H]indolyl-acetic acid to the apical bud ofVicia faba. Physiol. Plant.47, 260–268Google Scholar
  9. Bourbouloux, A., Bounemain, J.L., Vasseur, J. (1983) Caractéristiques du transport dans la tige de l'auxine-14C en provenance des jeunes feuilles deVicia faba L. C.R. Acad. Sci. Ser. D276, 501–504Google Scholar
  10. Catesson, A.M. (1981) Le cycle saisonnier des cellules cambiales chez quelques feuillus. Bull. Soc. Bot. Fr. Actual. Bot.128, 43–51Google Scholar
  11. Eschrich, W., Fritz, E. (1972) Microautoradiography of water soluble inorganic ions. In: Microautoradiography and electron probe analysis, pp. 99–112, Lüttge, U., ed. Springer, Berlin Heidelberg New YorkGoogle Scholar
  12. Everat-Bourbouloux, A. (1982) Transport et métabolisme de l'auxine et de l'acide abscissique chezVicia faba L. Thèse, PoitiersGoogle Scholar
  13. Goldsmith, M.H.M. (1977) The polar transport of auxin. Annu. Rev. Plant. Physiol.28, 439–478Google Scholar
  14. Jacobs, M., Gilbert, S.F. (1983) Basal localization of the presumptive auxin transport carrier in pea stem cells. Science220, 1297–1300Google Scholar
  15. Lachaud, S., Bonnemain, J.L. (1982) Xylogenèse chez les Dicotylédones arborescentes. III. Transport de l'auxine et activité cambiale dans les jeunes tiges de Hêtre. Can. J. Bot.60, 869–876Google Scholar
  16. Miginiac, E., Sossountzov, L., Dugue, N. (1978) Modalités du transport et du métabolisme de l'AIA-14C ou de l'AIA-3H en relation avec la morphogenèse des bourgeons axillaires chez leScrophularia argutd. Physiol. Plant.44, 335–344Google Scholar
  17. Morris, D.A., Kadir, G.O. (1972) Pathways of auxin transport in the intact pea seedling (Pisum sativum L.) Planta107, 171–182Google Scholar
  18. Morris, D.A., Thomas, A.G. (1978) A microautoradiographic study of auxin transport in the stem of intact pea seedlings (Pisum sativum L.). J. Exp. Bot.29, 147–157Google Scholar
  19. Nix, L.E., Wodzicki, T.J. (1974) The radial distribution and metabolism of IAA-14C inPinus echinata stems in relation to wood formation. Can. J. Bot.52, 1349–1355Google Scholar
  20. Sheldrake, A.R. (1973) Auxin transport in secondary tissues. J. Exp. Bot.24, 87–96Google Scholar
  21. Tvision, Y., Zamski, E. (1977) Translocation in plants possessing supernumerary phloem. II. The included phloem ofBougainvillea glabra Choisy and the bicollateral bundles of cucumber (Cucumis sativus L.). J. Exp. Bot.28, 127–131Google Scholar
  22. Wangermann, E. (1974) The pathway of transport of applied indolyl-acetic acid through internode segments. New Phytol.73, 623–636Google Scholar
  23. Wangermann, E., Withers, L.A. (1978) Auxin transport characteristics and cellular ultrastructure of different types of parenchyma. New Phytol.81, 1–17Google Scholar
  24. Zamski, E., Tvision, Y. (1977) Translocation in plants possessing supernumerary phloem. I.14C-assimilates and auxin in the internal phloem of tobacco (Nicotiana tabacum L.). J. Exp. Bot.28, 117–126Google Scholar
  25. Zamski, E., Wareing, P.F. (1974) Vertical and radial movement of auxin in young sycamore plants. New Phytol.73, 61–69Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Lachaud
    • 1
  • J. L. Bonnemain
    • 1
  1. 1.Laboratoire de Biologie et Physiologie végétales, Station Biologique de Beau-SiteFaculté des SciencesPoitiersFrance

Personalised recommendations