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Reversible, lectin-mediated immobilization of plant protoplasts on agarose beads

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Abstract

A technique is described for the reversible, lectin-mediated immobilization of plant protoplasts on agarose beads. Cyanogen-bromide-activated agarose beads were coated with protein (gelatine or bovine serum albumin) and lectins were subsequently linked to the protein layer using glutaraldehyde. The technique has possible applications in protoplast fusion-product isolation, cellrecognition studies, and membrane isolation.

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Abbreviations

BSA:

bovine serum albumin

Con A:

concanavalin A

FDA:

fluorescein diacetate

PNA:

peanut agglutinin

WGA:

wheat-germ agglutinin

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Authors and Affiliations

  1. Wolfson Institute of Biotechnology, The University, S10 2TN, Sheffield, UK

    Graham S. Warren & Rose Fallon

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  1. Graham S. Warren
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  2. Rose Fallon
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Warren, G.S., Fallon, R. Reversible, lectin-mediated immobilization of plant protoplasts on agarose beads. Planta 161, 201–206 (1984). https://doi.org/10.1007/BF00982913

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  • DOI: https://doi.org/10.1007/BF00982913

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